Fig 1: IL-22-/-/PyMT mice show downregulation of the gene expression of EMT-associated genes: (A–E) mRNA levels of Snail-1, Snail-2, Zeb-1, Twist-1, and MMP-3 genes in tumor cells purified from primary breast tumor tissues from 10-week-old IL-22+/+or IL-22-/-PyMT mice and quantitated by qPCR. n = 14 and 13, *P < 0.01. GAPDH was used as an endogenous control for normalization. The results are presented as mean ± SD. (F) WB showing MMP-3 protein expression in protein lysates prepared from purified tumor cells from primary breast tumor tissues of 10-week-old IL-22+/+or IL-22-/-PyMT mice. n = 3 per group. Protein concentrations were normalized using GAPDH.