PerCP anti-mouse CD4 100537 from BioLegend

Product Specs
ItemPerCP anti-mouse CD4
CompanyBioLegend
Price $99.00Supplier Page View Company Product Page
Catalog Number100537
NCBI Gene AliasesL3T4, Ly-4
Quantity25 µg
IsotypeRat IgG2a, kappa
ReactivityMouse
CloneRM4-5
ApplicationsFC - Quality tested
Conjugate/TagPERCP
HostRat
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.2mg/ml
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenBALB/c mouse thymocytes
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Recombinant Dabie bandavirus as a bivalent vaccine platform inducing protective immunity against intracellular pathogens and cancerMolecular TherapyFebruary 4, 2026Hyo-Jin Ro, Yebeen Lee, Kyeongseok Jeon, Yujin Kim, Seung Ho Baek, Green Kim, Joowan Kim, Jun-Gu Kang, Na-Yoon Jang, Si-Hyeon Lee, et al.against anti-CD3 Pacific Blue (clone 17-A2, BioLegend #100214), anti-CD4 Percp (clone RM4-5, BioLegend #100538), anti-CD8 FITC (clone 53-6.7, BioLegend #100706), Tetramer I APC (H-2K(b) chicken OVA 257-264 SIINFEKL;

(2) Noninvasive in vivo deoxycytidine kinase (dCK)-PET identifies tumor-draining lymph nodes upon immune checkpoint inhibitor therapynpj ImagingJanuary 6, 2026Cécile Philippe, Jonathan Cotton, Gregory D. Bowden, Simone Pöschel, Philipp Knopf, Barbara Schörg, Irene Gonzalez-Menendez, Dominik Sonanini, Lukas Flatz, Martin Allen-Auerbach, et al.following fluorescent monoclonal antibodies: (FITC)-CD3ε (1:100; #152304; BioLegend), (PerCP)-CD4 (1:25; #100538; BioLegend), (AF700®)-CD8a (1:100; #100730; BioLegend), (BV421™)-CD25 (1:100; #102043; BioLegend), (BV650™)-CD69

(3) CCL19-armed recombinant influenza virus inhibited colorectal cancer growth by remodeling tumor micro-environmentiScienceDecember 19, 2025Xia Ou, Yunxin Xia, Zhongyue Fang, Kai Yang, Guangtao Yang, Junying Wang, Yiyong Duan, Xiahui Yang, Bing Yang, Ze Liu, et al.PE anti-mouse CD3,Biolegend,100206 APC anti-mouse CD8a,Biolegend,100712 PerCP anti-mouse CD4,Biolegend,100538 PE anti-mouse CD25,Biolegend,102008 APC anti-mouse F4/80,Biolegend,123110 PE anti-mouse CD11b,Biolegend,101208 APC/Cyanine7 anti-mouse Ly-6G

(4) Recruiting T-cells toward the brain for enhanced glioblastoma chemo-immunotherapy efficacy by co-delivery of cytokines and temozolomide via ultrasound-gated redox-responsive extracellular vesiclesJ NanobiotechnologyDecember 7, 2025Lei Dong, Qing Xu, Hanjiao Liu, Ran Li, Zhengcheng Yun, Huijie Yang, Ziyan Gu, Junfan Wei, Haochun Guo, Zhanhai Wei, et al.anti-CD11c, anti-CD80, anti-CD86, anti-CD11b, anti-CD206 and anti-Foxp3 antibodies (103112, 100203, 100537, 100707, 100537, 100707, 100203, 100537, 100707, 100203, 100537, 126403, Biolegend, USA) for flow cytometry

(5) Heme and iron toxicity in the aged spleen impairs T cell immunity through iron deprivationNature AgingOctober 17, 2025David Ezuz, Heba Ombashe, Lana Watad, Akmaral Rakhymzhanova, Satyarth Pandey, Orna Atar, Esther G. Meyron-Holtz, Noga Ron-HarelPE anti-mouse CD45.1 (110707), PE anti-mouse CD69 (104508), PE anti-mouse IL-2 (503807), PerCP anti-mouse CD4 (100537), PerCP anti-mouse CD45 (103129) and purified anti-mouse CD16/32 (101302) (all from BioLegend); recombinant

(6) Oral Administration of Bovine Lactoferrin Modulates the Effects of Chronic Stress on the Immune Response of the LungsInt J Mol SciOctober 14, 2025Mariazell Yépez-Ortega, Erick José Zárate-Ayón, Crhistian Axel Gutiérrez-Calvillo, Belen Mendoza-Arroyo, Maritza Velásquez-Torres, Judith Pacheco-Yépez, Diana Rodríguez-Vera, María de Los Ángeles Gómez-Román, Uri Axel Garcia-Sanchez, Aldo Arturo Reséndiz-Albor, et al.BD Biosciences, anti-CD86 cat. 105012, BioLegend, San Diego, CA, USA), and T CD4+ cells (anti-CD4/PerCP cat. 100538, BioLegend). Subsequently, APCs were fixed with 4% paraformaldehyde. T lymphocytes and plasma cells

(7) ST8Sia6 overexpression protects pancreatic β cells from spontaneous autoimmune diabetes in nonobese diabetic miceJ Clin InvestAugust 1, 2025Justin Choe, Paul Belmonte, Sydney Crotts, Thanh Nguyen, David Friedman, Alexi Zastrow, Matthew Rajcula, Brady Hammer, Claire Wilhelm, Michael J. Shapiro, et al.F4/80-PE-Dazzle (BioLegend 123146 at 1:400), Gr1-PerCP (BioLegend 108426 at 1:500), CD4-PerCP (BioLegend 100538 at 1:500), PD-1–APC–Fire750 (BioLegend 135240 at 1:200), and PD-L1–BV421 (BioLegend 124315 at 1:100). Statistics. Figures

(8) Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interactionNat CommunJuly 3, 2025Zhen Fan, Ying Liu, Xueying Lin, Jifu Zhang, Jiehong Chen, Shiming Yi, Cheng Hu, Xincheng Liu, Cui Guo, Cuiying Xu, et al.53-6.7, 564983, BD; 53-6.7, 557654, BD), CD4 (RM4-5, 100536, BioLegend; RM4-5, 100510, BioLegend; RM4-5, 100538, BioLegend), NK1.1 (PK136, 562921, BD; PK136, 562062, BD), IL-18Rα (A17071D, 157906, BioLegend; A17071D,

(9) Impact of Sucrose Consumption on the Metabolic, Immune, and Redox Profile of Mice with Gestational Diabetes MellitusLife (Basel)June 20, 2025Cristian Ángel Rosales-Gómez, Beatriz Elina Martínez-Carrillo, Ana Laura Guadarrama-López, Aldo Arturo Reséndiz-Albor, Ivonne Maciel Arciniega-Martínez, Efrén Aguilar-Rodríguez553035, Franklin Lake, NJ, USA). Additionally, antibodies from BioLegend were used: CD4 (PerCP, Cat. No. 100538, San Diego, CA, USA), IL-1β (FITC, Cat. No. 506907, San Diego, CA, USA), IL-6 (APC, Cat. No. 504504,

(10) Fecal microbiota transplantation from Helicobacter pylori carriers following bismuth quadruple therapy exacerbates alcohol-related liver disease in mice via LPS-induced activation of hepatic TLR4/NF-κB/NLRP3 signalingJ Transl MedJune 3, 2025Huihui Gao, Hangming Bai, Yanyan Su, Yuting Gao, Hui Fang, Donghang Li, Youren Yu, Xing Lu, Dasheng Xia, Daqing Mao, et al.spleen, liver, and colon lamina propria. Cells were surface-stained with Percp-anti-mouse CD4 (BioLegend, 100537, U.S.) and APC-anti-mouse CD45 (BioLegend, 147707, U.S.), followed by intranuclear staining with PE-anti-mouse Foxp3

(11) Whole genome comparisons reveal gut-to-lung translocation of Escherichia coli and Burkholderia cenocepacia in two cases of ventilator-associated pneumonia in ICU patientsRespir ResMay 9, 2025Huihui Gao, Lei Xu, Yixin Liu, Xiaolong Wang, Siyuan Zhu, Huai Lin, Yuting Gao, Daqing Mao, Xing Lu, Yi Luousing FITC-anti-mouse CD3 (Thermo Fisher Scientific, 11-0032-80, USA), Percp-anti-mouse CD4 (BioLegend, 100537, USA), and Percp-anti-mouse CD45 (BioLegend, 103129, USA). For intracellular staining, cells were fixed

(12) Immunosuppressive microenvironment of liver restrains chemotherapeutic efficacy in triple-negative breast cancerJournal for ImmunoTherapy of CancerMarch 6, 2025Mingduo Liu, Mengjia Qian, Wen Sun, Xiaowei Sun, Yue Sun, Muxin Yu, Xinyu Tang, Xinrui Mao, Chang Sun, Qi Qi, et al.Alexa Fluor 700 anti-mouse CD8a Antibody (Biolegend, Cat# 100729), PerCP anti-mouse CD4 Antibody (Biolegend, Cat# 100537), PE anti-mouse/human CD44 Antibody (Biolegend, Cat# 103007), Brilliant Violet 605 anti-mouse CD62L

(13) Boosting tumor homing of endogenous natural killer cells via therapeutic secretomes of chemically primed natural killer cellsJ Immunother CancerMarch 5, 2025Seohyun Cho, Seung Hee Choi, Eunchong Maeng, Hail Park, Ki Seo Ryu, Kyung-Soon ParkAPC/Cyanine7-mNK1.1 (108724), APC-mNKp46 (137608), Brilliant Violet 421-mCD3 (100228), PerCP-mCD4 (100538), and Alexa Fluor 700-mCD8a (100730). All mice were purchased from JA Bio (Gyeonggi-do, Republic of

(14) NFAT single-deficient murine T cells reduce the risk of aGvHD while controlling cytomegalovirus infectioniScienceFebruary 21, 2025Nadine Hundhausen, Snigdha Majumder, Yin Xiao, Sigrun S. Haeusl, Helen Goehler, Rishav Seal, Cristina M. Chiarolla, Andreas Rosenwald, Matthias Eyrich, Luka Cicin-Sain, et al.RRID:AB_493647 anti-mouse CD4 (GK1.5),BioLegend,Cat#: 100422; RRID:AB_312707 anti-mouse CD4 (RM4:5),BioLegend,Cat#: 100538; RRID:AB_893325 anti-mouse CD8α (53-6.7),BioLegend,Cat#: 100752; RRID:AB_2563057 anti-mouse CD8α (53-6.7),BioLegend,Cat#:

(15) Hspa13 Deficiency Impaired Marginal Zone B Cells Regulatory Function and Contributed to Lupus PathogenesisAdvanced ScienceDecember 31, 2024Chen Xing, Haoran Cui, Ge Li, Xiaoling Liu, Kun Liu, Qing Wen, Xin Huang, Renxi Wang, Lun SongBioLegend), PerCP‐conjugated anti‐mouse B220 (103233, BioLegend), PerCP‐conjugated anti‐mouse CD4 (100537, BioLegend), APC‐conjugated anti‐mouse B220 (103211, Biolegend), PE/Cyanine7‐conjugated anti‐mouse IL‐10

(16) Charge-assisted stabilization of lipid nanoparticles enables inhaled mRNA delivery for mucosal vaccinationNature CommunicationsNovember 2, 2024Shuai Liu, Yixing Wen, Xinzhu Shan, Xinghuan Ma, Chen Yang, Xingdi Cheng, Yuanyuan Zhao, Jingjiao Li, Shiwei Mi, Haonan Huo, et al.CD4 + TRM cells, the following antibodies were used: PerCP anti-mouse CD4 antibody (BioLegend, Cat. 100537), FITC anti-mouse CD44 antibody, PE anti-mouse CD69 antibody, and APC anti-mouse CD11a/CD18 antibody

(17) T-follicular regulatory cells expand to control germinal center plasma cell output but fail to curb autoreactivityiScienceOctober 18, 2024Cecilia Fahlquist-Hagert, Thomas Rea Wittenborn, Mattias Krogh Pedersen, Lisbeth Jensen, Søren Egedal DegnAB_2738574 "Anti-CD38-PE-Cy7, clone 90",BioLegend,Cat#102718; RRID: AB_2275531 "Anti-CD4-PerCP, clone RM4-5",Biolegend,Cat#100538; RRID: AB_893325 "Anti-CD4-Qdot605, clone RM4-5",ThermoFisher Scientific,Cat#Q10092; RRID: AB_10374736 "Anti-CD8-PerCP-Cy5.5,

(18) All-Trans-Retinoic Acid-Adjuvanted mRNA Vaccine Induces Mucosal Anti-Tumor Immune Responses for Treating Colorectal CancerAdvanced science (Weinheim, Baden-Wurttemberg, Germany)June 1, 2024Li W, Li Y, Li J, Meng J, Jiang Z, Yang C, Wen Y, Liu S, Cheng X, Mi S, et al.mouse anti‐CD3‐FITC (BioLegend, 100204), anti‐CD4‐peridinin‐chlorophyll‐protein (PerCP) (Biolegend, 100537), anti‐CCR9‐ phycoerythrin (PE) (BioLegend, 129708), and anti‐α4β7‐allophycocyanin (APC) (Biolegend,

(19) Identification and affinity enhancement of T-cell receptor targeting a KRASG12V cancer neoantigenCommunications BiologyApril 29, 2024Mengyu Zhang, Wei Xu, Lingjie Luo, Fenghui Guan, Xiangyao Wang, Pei Zhu, Jianhua Zhang, Xuyu Zhou, Feng Wang, Sheng Yeantibodies were used: FITC-anti-mCD8a (53-6.7, cat. no. 100706, Biolegend), Percp-anti-mCD4 (RM4-5, cat. no. 100538, Biolegend); APC-anti-mIFNγ (XMG1.2, cat. no. 505810, Biolegend). Single-cell V(D)J + 5’ from CD8+

(20) A Pvs25 mRNA vaccine induces complete and durable transmission-blocking immunity to Plasmodium vivaxNPJ VaccinesDecember 14, 2023Nawapol Kunkeaw, Wang Nguitragool, Eizo Takashima, Niwat Kangwanrangsan, Hiromi Muramatsu, Mayumi Tachibana, Tomoko Ishino, Paulo J. C. Lin, Ying K. Tam, Sathit Pichyangkul, et al.Then cells were washed and surface stained with anti-mouse CD3 (BioLegend 100306) and CD4 (BioLegend 100538) antibodies. Following surface staining, cells were washed in Fix/Perm buffer (BD Biosciences 51-2090KZ)

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12/2/2015Excellent Antibody for Staining CD4+ T Cells Chantal KuhnOur laboratory is very interested in the role of regulatory T cells in autoimmunity. We mainly focus on regulatory CD4+FoxP3+ T cells and CD4+FoxP3-LAP+ T cells.

PerCP anti-mouse CD4 from BioLegend

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PerCP anti-mouse CD4 from BioLegend

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