Fig 1: Cell-cell interactions between T14_HLA-DR+ cells and other T cells. A and B Co-localization between T14_HLA-DR+ cells and exhausted CD4+ (A) or CD8+ (B) T cells in spatial transcriptomics data from CORE (top) and LUNG (bottom) cancers. Localized magnified regions are counterstained with Hematoxylin-eosin (HE). Scale bars, 200 μm. C Chord plot illustrating ligand-receptor pairs from consensus databases compiled by the LIANA software. Arrows point from the ligand toward the receptors, with only HLA class-II pairs highlighted. D and E Spatial mapping of MHC class-II-CD4 (D) and MHC class-II-LAG3 (E) pairs gene expression on spatial transcriptome slides from CORE (top) and LUNG (bottom) cancers. MHC class-II (HLA-DRB1, HLA-DPB1, HLA-DRA, HLA-DPA1, HLA-DQB1, HLA-DQA1) and CD4/LAG3 pairs as identified in Fig. 5C. F In the BLCA ICB cohort, patients stratified by T14_HLA-DR+ abundance showed significant differences in response rates. T14_HLA-DR+ high group (31.15%) had a significantly higher response rate than the low group (17.05%). The p-value was calculated by Fisher’s exact test. G, (H) and (I) Kaplan-Meier overall survival curves for the BLCA (G), SKCM Gide (H) and SKCM Riaz (I) ICB cohorts grouped by T14_HLA-DR+ cells abundance. The p values were calculated by log-rank test