Fig 2: CHPF is associated with EMT relative markers and MMP2. (A–D) The GEPIA database was used to analyze the relationship between CHPF and metastatic markers, including vimentin, Snail, Slug and MMP2. (0 < r < 1, Pearson correlation coefficient). (E, F) Effects of CHPF depletion (E) or overexpression (F) on motion-related markers in MCF7 (E) or MDA-MB-231 (F) cells were determined by western blotting. **P < 0.01 vs. si-con group or vector group. Data are presented as the mean ± SD for three independent experiments. Statistical analyses were performed using Student's t test.

Fig 3: Calreticulin Absence Reduces S9 Phosphorylation of GSK3ß and Affects SNAIL2/SLUG Nuclear Translocation(A) qPCR analysis shows the mRNA expression of TGF-ß receptor markers TgfbR1, TgfbR2, and TgfbR3 in WT and CRT-KO cells during differentiation.(B) N-cadherin, E-cadherin, pAKT (S473), and pGSK3ß (S9) versus total GSK3ß and total AKT were examined in WT, CRT-KO, and CN cells at D14 of cardiac differentiation from total cell lysates by western blot analysis. Compared with calreticulin-containing WT cells, in KO cells E-cadherin is more abundant, while in contrast N-cadherin is more abundant in WT cells than in calreticulin-deficient KO cells. CN cells express less E-cadherin and more N-cadherin. AKT phosphorylation at serine 473 and GSK3ß phosphorylation at S9 is low in CRT-KO cells.The bar graphs show the quantification of E-cad/N-cad, pAKT, and pGSK3ß band density versus GAPDH from three independent experiments; *p = 0.05, **p = 0.01, ***p = 0.001, ****p = 0.0001.(C) Western blot detection of SNAIL2/SLUG in nuclear fractions of in WT, CRT-KO, and CN cells during cardiac differentiation in D10 and D14.