Fig 2: COMET identifies favorable markers for splenic follicular B cells Clustering and t‐SNE visualization of splenic B cells as generated by Tabula Muris (Tabula Muris Consortium, 2018).Expression of follicular B‐cell marker CD23 and marginal zone B‐cell marker CD21 in the splenic B‐cell dataset from Tabula Muris as visualized by COMET.Expression of the three top‐ranking markers for follicular B‐cell output by COMET, CD55, CD62L, and CXCR4, as visualized by COMET.Flow cytometry analysis comparing the protein level staining of CD23, an established marker for follicular B cells, with the three top‐ranking marker genes in the COMET output confirms that COMET's top‐ranking candidate markers are favorable for flow cytometry staining of the follicular B‐cell subtype. The genes to validate were selected based on availability of trustable antibodies (SP = single positive). Bars and error bars indicate the mean and standard deviation. ****P < 0.0001; n = 6 biological replicates; unpaired, two‐tailed t‐test.

Fig 3: Alterations of B-cell subset and pro-inflammatory cytokine expression in LIP-MI mice following C1.5P4/BMP-2 hydrogel therapy. (A-B) Representative flow cytometry plots of CD5-B220+ cells (arrow), CD5−CD23+B220+ (B2) cells (in periodontal tissues and hearts. (C-D) Representative flow cytometry plots of TNF-α and IL-6 positive cells in CD5−CD23+ B2 cells. (E-H) Quantitative analysis of B-cell subsets and cytokine positive cells in periodontal tissues and hearts. (I-J) Immunofluorescence staining of CD23 (B2 cell marker) and TNF-α in periodontium and hearts. N = 6 for for all panels. ∗p < 0.05 compared to LIP-MI group. N.S., not significant.