Fig 1: Quantitative analysis of the immunohistochemical stainings of antimicrobial peptide expression. (A) In polymorphic light eruption (PLE), the number of psoriasin-positive cells in the epidermis was significantly higher than in healthy skin but slightly lower compared to that in psoriasis. (B) Expression of RNase7 in PLE was significantly higher than compared to that of healthy skin, atopic dermatitis (AD) or psoriasis. (C) HBD-2 was significantly increased in PLE and psoriasis and to a lower degree increased in AD compared to healthy skin. (D) The expression of HBD-3 was (significantly) decreased in PLE compared to AD, psoriasis, and healthy skin. (E) Similar to psoriasis, PLE showed significantly increased expression of LL37 compared to healthy skin and AD. Data presented as mean with SEM
Fig 2: Immunohistochemical staining reveals altered antimicrobial peptide expression in polymorphic light eruption. Representative images of immunohistochemically stained tissue sections (counterstained with hematoxylin). Polymorphic light eruption (PLE), atopic dermatitis (AD), and psoriasis samples were stained for psoriasin (A-D), RNase7 (E-H), HBD-2 (I-L), HBD-3 (M-P), and LL37 (Q-T). Healthy, normal-appearing human skin was used as a control. PLE showed increased expression of Psoriasin (B) and RNAse7 (F) both mostly in the stratum granulosum of the epidermis; HBD-2 was mostly expressed in the cellular infiltrate in the dermis (J) and LL37 in and around blood vessels and glands (R), whereas HBD-3 (N) was decreased in epidermis and dermis. A similar expression profile is observed in lesional psoriatic skin, different to that of lesional skin of AD (with little or no expression of psoriasin, RNAse7, HBD-2 and LL37 and upregulation of HBD-3). Original magnification of psoriasin, RNase7, HBD-3, and LL37: ×200 and HBD-2: ×100
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