Fig 1: The hyperactivity behavior of Pex7 deficient mice in the open field environment. Pex7 deficient mice showed significant increase in activity levels measured by distance traveled (A) and activity time (B) at different ages in comparison to controls (Pex7 WT/WT and Pex7 WT/hypo). Representative images of track blots recorded during the open field test of Pex7 deficient mice and their littermate controls (C). Bars represent group means ± SD. ns: nonsignificant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (n = 12–36 per genotype).
Fig 2: Pex7 genotype correlates with survival rates and weight gain. Kaplan–Meier plots showing the percentage of survival of Pex7 deficient mice (n = 41–60 per genotype). Analyzing the survival data by log rank tests showed significant differences between genotypes (p < 0.001) in accordance to the severity of Pex7 genotype (A). Graded and significant reduction in body weight according to Pex7 genotype severity is found in Pex7 mutants compared to controls at 1, 4, and 12 months (M) of age (n = 10–12 per genotype) (B). ns: nonsignificant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Fig 3: Pex7 genotype correlates with the myelin content in brain tissues from 12-month-old Pex7 deficient mice. Sagittal sections of brain tissues: corpus callosum (A–D) and cerebellum (E–H) show graded reduction in the levels of anti-MBP immunohistochemical staining in 12-month-old Pex7 deficient mice compared to their littermate controls. Slides were counterstained with hematoxylin (I, J) (Scale bar = 500 μm). Graded reduction in the amount of MBP protein levels was found in the immunoblot and densitometric analysis of cerebellar homogenates from Pex7 deficient mice at 12 months of age (K, L). (n = 3 per genotype).
Fig 4: Pex7 genotype correlates with C26:0-LPC (VLCFA) levels. Elevations in C26:0-LPC represented as fold change over wild-type (WT) are detected in the blood and several tissues of 4-month-old Pex7 deficient mice. No significant increase in C26:0-LPC levels was found in the cerebral cortex of Pex7 deficient mice (n = 4–6 per genotype). The dotted line represents the C26:0-LPC levels in Pex7 WT/WT mice. ns: nonsignificant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Fig 5: Brain neurotransmitter deficits correlate with activity levels in Pex7 deficient mice. A significant increase in activity levels of a subset of Pex7 deficient mice is confirmed before neurotransmitters analysis (A). A significant reduction in dopamine, serotonin, norepinephrine and GABA neurotransmitters is observed in cortical brain tissues from Pex7 deficient mice compared to littermate controls (Pex7 WT/WT and Pex7 WT/hypo) (B). The hyperactive phenotype in Pex7 deficient mice is inversely correlated with brain neurotransmitter levels (C) and total PlsEtn levels in cerebral cortex as well as plasma (D). Bars represent group means ± SD. ns: nonsignificant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (n = 8–10 per genotype).
Supplier Page from Abcam for Anti-PEX7 antibody