Notch-1 Intracellular Domain Antibody AF3647 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemNotch-1 Intracellular Domain Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF3647
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,CyTOF-ready
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
ReactivityHuman
TargetNotch-1
HostSheep
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Notch-1, Gly2428-Lys2556, Accession # P46531
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namenotch receptor 1
NCBI Gene AliaseshN1, AOVD1, AOS5, TAN1

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects the intracellular domain (ICD) of human Notch-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Notch-2 ICD, rhNotch-3 ICD, and rhNotch-4 ICD is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Somatic mutations reveal hyperactive Notch signaling in prurigo nodularisJCI InsightApril 22, 2026Ahmad Rajeh, Shahin Shahsavari, Hannah Cornman, Alexander Kollhoff, Anuj Gupta, Mindy D. Szeto, Anusha Kambala, Olusola O. Oladipo, Varsha Parthasarathy, Junwen Deng, et al.slides were then incubated with primary antibodies for NOTCH1 (ab52627, Abcam; 1:150) and human NICD (AF3647, R&D Systems; 1:20) at 4°C overnight, followed by reaction with conjugated secondary antibodies, DAPI

(2) BCAT1 is a NOTCH1 target and sustains the oncogenic function of NOTCH1HaematologicaFebruary 1, 2025Valeria Tosello, Ludovica Di Martino, Adonia E. Papathanassiu, Silvia Dalla Santa, Marco Pizzi, Lara Mussolin, Jingjing Liu, Pieter Van Vlierberghe, Erich Piovanimmunoprecipitated with human Notch-1 intracellular domain antibody (R&D Systems, Minneapolis, MN; Cat#AF3647). Normal sheep immunoglobulin G (IgG) (Cat#5-001-A, R&D Systems) was used as a non-specific antibody

(3) Somatic mutations reveal hyperactive Notch signaling and racial disparities in prurigo nodularismedRxiv : the preprint server for health sciencesSeptember 26, 2023Rajeh A, Cornman HL, Gupta A, Szeto MD, Kambala A, Oladipo O, Parthasarathy V, Deng J, Wheelan S, Pritchard T, et al.slides were then incubated with primary antibodies for Notch1 (ab52627, Abcam, 1:150) and human NICD (AF3647, R&D Systems; dilution, 1:20) at 4°C overnight, followed by reaction with conjugated secondary antibodies,

(4) Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cellsThe Journal of experimental medicineMarch 4, 2019Chen HJ, Poran A, Unni AM, Huang SX, Elemento O, Snoeck HW, Varmus H.(Cell Signaling; clone D3B8, cat. no. 4147), anti-NOTCH1 or NICD (R&D Systems; clone 2D9, cat. no. AF3647), anti-NOTCH2 (Cell Signaling; clone 8a1, cat. no. 2420), anti-Ki67 (Cell Signaling; clone D2H10, cat.

(5) Image-guided genomics of phenotypically heterogeneous populations reveals vascular signalling during symbiotic collective cancer invasionNature communicationsMay 12, 2017Konen J, Summerbell E, Dwivedi B, Galior K, Hou Y, Rusnak L, Chen A, Saltz J, Zhou W, Boise LH, et al.antibody (1:1,000) and VEGF-neutralizing antibody (1:1,000) were obtained from R&D Systems (Cat. nos. AF3647 and MAB293). Primary antibodies against Dll4 (1:500) and VEGFA (1:500) were obtained from Santa Cruz

(6) A Notch positive feedback in the intestinal stem cell niche is essential for stem cell self-renewalMolecular systems biologyApril 28, 2017Chen KY, Srinivasan T, Tung KL, Belmonte JM, Wang L, Murthy PKL, Choi J, Rakhilin N, King S, Varanko AK, et al.,Santa Cruz Biotechnology,sc‐6011,1:500 (WB) Anti‐lysozyme,Abcam,ab108508,1:100 (IF) Anti‐human NICD,R&D Systems,AF3647,1:200 (ChIP) Anti‐mouse NICD,Cell Signaling,4147,1:200 (ChIP) Anti‐NOTCH1,Santa Cruz Biotechnology,sc‐9170,"1:1,000

(7) HIV-host interactome revealed directly from infected cellsNature microbiologyJuly 1, 2016Luo Y, Jacobs EY, Greco TM, Mohammed KD, Tong T, Keegan S, Binley JM, Cristea IM, Fenyö D, Rout MP, et al.(AV33117; Sigma-Aldrich); a sheep polyclonal antibody to the Notch1 C-terminal domain (aa 2428–2556) (AF3647; R&D Systems); a rabbit mAb specifc for the Notch1 NICD (V1744) (D3B8; Cell Signalling). The latter

(8) A dual molecular analogue tuner for dissecting protein function in mammalian cellsNature communicationsMay 27, 2016Brosh R, Hrynyk I, Shen J, Waghray A, Zheng N, Lemischka IR.catalogue number sc-7389, 1:250), sheep anti-Notch-1 Intracellular Domain (R&D Systems, catalogue number AF3647, 1:200) and mouse anti-α-Tubulin (Sigma, catalogue number T9026, 1:1,500). Quantification of protein

(9) AMPA-Kainate Receptor Inhibition Promotes Neurologic Recovery in Premature Rabbits with Intraventricular HemorrhageThe Journal of neuroscience : the official journal of the Society for NeuroscienceMarch 16, 2016Dohare P, Zia MT, Ahmed E, Ahmed A, Yadala V, Schober AL, Ortega JA, Kayton R, Ungvari Z, Mongin AA, et al.Sox9 (catalog #ab5535, Millipore), and sheep polyclonal Notch intracellular domain (NICD) (catalog #AF3647, R&D Systems). Secondary antibodies used were Cy-3 conjugate donkey anti-mouse, Cy-3 conjugate donkey

(10) Involvement of Notch in activation and effector functions of γδ T cellsJournal of immunology (Baltimore, Md. : 1950)March 1, 2014Gogoi D, Dar AA, Chiplunkar SV.

(11) Delta-like 4 inhibits choroidal neovascularization despite opposing effects on vascular endothelium and macrophagesAngiogenesisDecember 1, 2012Camelo S, Raoul W, Lavalette S, Calippe B, Cristofaro B, Levy O, Houssier M, Sulpice E, Jonet L, Klein C, et al.incubated in PBS diluted antibodies. The following primary antibodies were used: human/mouse Notch1 (AF3647, R&D systems, France), rabbit polyclonal anti human/mouse Notch4 (ab23427, Abcam, Cambridge, UK), rabbit

(12) NOTCH1-Dependent Nitric Oxide Signaling Deficiency in Hypoplastic Left Heart Syndrome Revealed Through Patient-Specific Phenotypes Detected in Bioengineered CardiogenesisStem CellsJanuary 1, 0001"Hrstka SC, Li X, Nelson TJ, Wanek Program Genetics Pipeline Group.."

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Human Notch-1 Intracellular Domain Antibody

Notch-1 Intracellular Domain Antibody

Notch-1 Intracellular Domain Antibody

Notch-1 Intracellular Domain Antibody

Notch-1 Intracellular Domain Antibody

Notch-1 Intracellular Domain Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (12)

Reviews

Notch-1 Intracellular Domain Antibody from R&D Systems, a Bio-Techne Brand

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