Fig 2: Functionality and specificity of MYD88L265P-specific T cells. Functionality and specificity of MYD88L265P-specific CD8+ T cells were analyzed by (A) IFNγ ELISPOT assay or (B, C) intracellular cytokine staining. Both assays showed increased production of IFNγ or TNFα after stimulation with the mutation-derived peptide (P1B*07) in comparison with the corresponding WT peptide (P1WT). Representative examples of two different donors are shown. The frequency of P1B*07-specific CD8+ T cell populations was 2.69% (A) and 0.40% (B and C), respectively, as detected by tetramer staining (not shown). Error bars indicate ± SEM of two independent replicates. Abbreviations: SFU, spot forming unit; neg., negative; pos., positive; SEM, standard error of the mean.

Fig 3: Comparison of circulating CD8+ T cell subpopulations between different treatments in patients with NMOSD. The proportions of CD8+ T subpopulations from NMOSD patients under different treatments, including 17 non‐treatment (NT), 22 glucocorticoids (GC), and 18 immunosuppressive treatments (IST). TN, naïve T cells; TE/M, effector/memory T cells; TMP, memory precursor T cells; TSLEC, short lived effector T cells. **P < 0.01, and ns (not significant) by Kruskal–Wallis test with Dunn's correction for multiple comparison test. Each P value was corrected for age and sex.

Fig 4: Immunohistochemistry of high-grade serous OvCa. Immunohistochemical stainings on TMAs with 136 high-grade serous OvCa samples were performed. (A) Expression of HDAC1: Top: Immunohistochemical staining for HDAC1 of two exemplary OvCa samples with high HDAC1 expression (score 12, left) and low HDAC1 expression (score 3, right). Bottom: Impact of HDAC1 on OS of high-grade serous OvCa stage III and IV (Kaplan Meier analysis, low expression: score < 9, n = 94; high expression score ≥ 9, n = 45). (B) Intraepithelial CD3+ TILs: Top: Immunohistochemical staining for CD3+ TILs of two exemplary OvCa samples with lower infiltration (left) and higher infiltration (right) of TILs (epithelial compartment is highlighted in yellow). Bottom: Impact of epithelial CD3+ TILs on OS of high-grade serous OvCa stage II to IV (Kaplan Meier analysis, low: < 7 cells/HPF, n = 108; high: ≥ 7 cells/HPF, n = 28). (C) Intrastromal CD8+ TILs: Top: Immunohistochemical staining for CD8+ TILs of two exemplary OvCa samples with lower infiltration (left) and higher infiltration (right) of TILs (stromal compartment is highlighted in green). Bottom: Impact of stromal CD8+ TILs on OS of high-grade serous OvCa stage II to IV (Kaplan Meier analysis, low: < 1.5 cells/HPF, n = 91; high: ≥ 1.5 cells/HPF, n = 45).

Fig 5: CD3− CD56+ cells are associated with reduced TIL growth, whereas CD4+ T cells are preferentially found in faster-growing cultures. ILC (CD3−CD56+) (a, b), CD4+ (c, d), CD8+ (e, f), and CD4−CD8− (g, h) subsets were quantified in TIL cultures according to their rates of expansion (faster or slower). Analysis was performed for malignant and nonmalignant TIL cultures as indicated. Two-tailed Mann-Whitney tests were used for A-H.