PTP1B Antibody AF3954 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPTP1B Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF3954
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Frozen,Simple Western
ReactivityHuman,Mouse,Rat
HostGoat
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
Quantity100 ug (also 25 ug)
TargetPTP1B
ClonalityPolyclonal
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
Antibody TypePrimary Antibodies
PurityAntigen Affinity-purified
ConcentrationLYOPH
ImmunogenE. coli-derived recombinant mouse PTP1B, Met1-Phe431, Accession # P35821
NCBI Full Gene Nameprotein tyrosine phosphatase, non-receptor type 1, protein tyrosine phosphatase non-receptor type 1
NCBI Gene AliasesPTP1B, PTP-1B, PTP-HA2

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse and rat PTP1B in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human PTP1B is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) JAK2/STAT3 Signaling Pathway Modulates Acute Methylmercury Toxicity in the Mouse Astrocyte C8-D1A Cell LineNeurochem ResAugust 13, 2025Aafia Ahmed, Maximus Wong, Abel Santamaria, João Batista Rocha, Aaron B Bowman, Michael Aschner, Beatriz Ferrer(R&D systems, MAB3024), rabbit anti-SOD2 (Cell Signaling Technology, #813141), goat anti-PTP1B (R&D systems, AF3954), and mouse anti-HO1 (Cell Signaling Technology, #86806). Quantitative Real time PCR Cells were cultured

(2) Mechanistic insights into a heterobifunctional degrader-induced PTPN2/N1 complexCommunications ChemistryAugust 16, 2024Qi Hao, Manoj K. Rathinaswamy, Kelly L. Klinge, Matthew Bratkowski, Amirhossein Mafi, Christina K. Baumgartner, Keith M. Hamel, Gesine K. Veits, Rinku Jain, Claudio Catalano, et al.(D7T7D) Rabbit mAb (Cell Signaling, Danvers, 58935), anti-mouse PTP1B goat pAb (R&D Systems, Minneapolis, AF3954), and anti-vinculin mouse mAb (MilliporeSigma, Burlington, SAB4200080). Secondary antibodies used include

(3) Protein Tyrosine Phosphatase 1B Deficiency Improves Glucose Homeostasis in Type 1 Diabetes Treated With LeptinDiabetesSeptember 1, 2022Ito Y, Sun R, Yagimuma H, Taki K, Mizoguchi A, Kobayashi T, Sugiyama M, Onoue T, Tsunekawa T, Takagi H, et al.Triton X-100, 10% BSA [Wako] in PBS) and then overnight at 4°C in goat anti-PTP1B (1/100 dilution, AF3954; R&D Systems) in blocking buffer. After washing with PBS, sections were treated with Alexa Fluor 488–conjugated

(4) PTP1B Is an Intracellular Checkpoint that Limits T-cell and CAR T-cell Antitumor ImmunityCancer discoveryMarch 1, 2022Wiede F, Lu KH, Du X, Zeissig MN, Xu R, Goh PK, Xirouchaki CE, Hogarth SJ, Greatorex S, Sek K, et al.RRID:AB_2877148) antibody for flow cytometry was from Abcam; and goat polyclonal PTP1B (catalog no. AF3954, RRID:AB_2174947) antibody for immunoblotting was from R&D Systems. Recombinant human IL2, murine IL7,

(5) Comprehensive analysis of PTPN family expression and prognosis in acute myeloid leukemiaFrontiers in geneticsJanuary 1, 2022Liu Y, Zhang J, Du Z, Huang J, Cheng Y, Yi W, Li T, Yang J, Chen C.against β-actin were from Sigma-Aldrich (Mouse, A5441, Shanghai, China). Antibodies against PTPN1(Goat, AF3954) was from Novus Biologicals (Littleton, CO). Antibodies against PTPN7(Goat, AF3954) was from Novus Biologicals

(6) The antioxidant role of STAT3 in methylmercury-induced toxicity in mouse hypothalamic neuronal GT1-7 cell lineFree radical biology & medicineAugust 1, 2021Ferrer B, Suresh H, Santamaria A, Rocha JB, Bowman AB, Aschner M.(Cell Signaling Technology #3230), rabbit anti-SOCS3 (Cell Signaling Technology, #2932), goat anti-PTP1B (R&D Systems, AF3954), rabbit anti-SOD2 (Cell Signaling Technology, #13141), mouse anti-HO1 (Santa Cruz Biotechnology, sc-390991),

(7) Chronic exposure to methylmercury enhances the anorexigenic effects of leptin in C57BL/6J male miceFood and chemical toxicology : an international journal published for the British Industrial Biological Research AssociationJanuary 1, 2021Ferrer B, Prince LM, Tinkov AA, Santamaria A, Farina M, Rocha JB, Bowman AB, Aschner M.(Cell Signaling Technology, #3230), rabbit anti-SOCS3 (Cell Signaling Technology, #2932), goat anti-PTP1B (R&D Systems, AF3954), rabbit anti-phospho-AKT (Ser473) (Cell Signaling Technology, #9271), rabbit anti-AKT (Cell Signaling Technology

(8) Hepatic protein-tyrosine phosphatase 1B disruption and pharmacological inhibition attenuate ethanol-induced oxidative stress and ameliorate alcoholic liver disease in miceRedox biologySeptember 1, 2020Hsu MF, Koike S, Mello A, Nagy LE, Haj FG.(ab129068), NOX4 (ab216654) and 4-hydroxynonenal (4-HNE, ab46545) were from Abcam. Antibodies for murine (AF3954) and human (MABS197) PTP1B were from R&D Systems and Millipore, respectively. Information on the used

(9) Intranasal Targeting of Hypothalamic PTP1B and TCPTP Reinstates Leptin and Insulin Sensitivity and Promotes Weight Loss in ObesityCell reportsSeptember 10, 2019Dodd GT, Xirouchaki CE, Eramo M, Mitchell CA, Andrews ZB, Henry BA, Cowley MA, Tiganis T.

(10) Adrenalectomy impairs insulin-induced hypophagia and related hypothalamic changesThe Journal of endocrinologyAugust 1, 2019Uchoa ET, Marangon PB, Rorato R, Ruginsk SG, Debarba LK, Antunes-Rodrigues J, Elias LLK.Cell Signaling # 9252), rabbit anti-phospho JNK (1:1000, Cell Signaling # 9251), goat anti-PTP1B (0,15 µg/mL, AF3954 – R&D Systems) and rabbit antiTCPTP (1:3000, AB180714-Abcam). The blots were rinsed in TBS-T and then

(11) Pathologic Oxidation of PTPN12 Underlies ABL1 Phosphorylation in Hereditary Leiomyomatosis and Renal Cell CarcinomaCancer researchDecember 1, 2018Xu Y, Taylor P, Andrade J, Ueberheide B, Shuch B, Glazer PM, Bindra RS, Moran MF, Linehan WM, Neel BG.and analyzed by immunoblotting. Antibodies included: oxidized PTP (335636) and anti-PTP1B antibodies (AF3954) from R&D Systems; anti-ERK2 (D-2), anti-FH (H-6), anti-SHP2 (C-18 and B1) and anti-ABL1 (8E9) antibodies

(12) Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCCCellNovember 15, 2018Grohmann M, Wiede F, Dodd GT, Gurzov EN, Ooi GJ, Butt T, Rasmiena AA, Kaur S, Gulati T, Goh PK, et al.mAb","N. K. Tonks, Cold Spring Harbor Laboratory, NY",N/A "PTP1B, polyclonal goat Ab",R&D Systems,Cat# AF3954; RRID:AB_2174947 "Oxidized PTP active site, clone 335636, mouse mAb",R&D Systems,Cat# MAB2844; RRID:AB_664170 "Phospho-Stat1

(13) Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2Nature communicationsSeptember 6, 2017Tsutsumi R, Harizanova J, Stockert R, Schröder K, Bastiaens PIH, Neel BG.chicken polyclonal anti-GFP (ab13970) antibodies were purchased from Abcam. Goat polyclonal anti-PTP1B (AF3954) and anti-PDGFRβ (AF1042) antibodies were purchased from R&D Systems. Mouse anti-FLAG antibody (M2,

(14) Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in MicrogliaEBioMedicineFebruary 1, 2017Tsunekawa T, Banno R, Mizoguchi A, Sugiyama M, Tominaga T, Onoue T, Hagiwara D, Ito Y, Iwama S, Goto M, et al.

(15) Ventromedial hypothalamus-specific Ptpn1 deletion exacerbates diet-induced obesity in female miceThe Journal of clinical investigationSeptember 1, 2014Chiappini F, Catalano KJ, Lee J, Peroni OD, Lynch J, Dhaneshwar AS, Wellenstein K, Sontheimer A, Neel BG, Kahn BB.0.1% BSA in 0.1 M PBS) and then incubated for 24 hours at RT with goat anti-PTP1B antibodies (1:100) (AF3954; R&D Systems Inc.) diluted in 2% donkey serum (in 0.2% Triton X-100, 0.1% BSA in 0.1 M PBS). Sections

(16) Targeting density-enhanced phosphatase-1 (DEP-1) with antisense oligonucleotides improves the metabolic phenotype in high-fat diet-fed miceCell communication and signaling : CCSJuly 26, 2013Krüger J, Trappiel M, Dagnell M, Stawowy P, Meyborg H, Böhm C, Bhanot S, Ostman A, Kintscher U, Kappert K.final concentration of 50 mM. For immunoprecipitation PTP analyses anti-DEP-1 and anti-PTP1B (AF1934 and AF3954 R&D Systems, Wiesbaden, Germany) were used at a concentration of 1 μg in an end-over-end reaction at

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PTP1B Antibody

PTP1B Antibody

PTP1B Antibody

PTP1B Antibody

PTP1B Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (16)

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PTP1B Antibody from R&D Systems, a Bio-Techne Brand

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