COX-2 Antibody AF4198 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCOX-2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF4198
HostGoat
ReactivityHuman,Mouse,Xenograft
TargetCOX-2
Quantity100 ug (also 25 ug)
NCBI Full Gene Nameprostaglandin-endoperoxide synthase 2
ApplicationsWB, FCM, ICC, IHC, IHC-fr, IHC-p, MC
ClonalityPolyclonal
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ImmunogenE. coli-derived recombinant human COX-2, Ala18-Leu604, Accession # P35354
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
NCBI Gene AliasesPHS-2, PGHS-2, COX2, PGG/HS, COX-2, GRIPGHS, hCox-2

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse COX-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human COX-1 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this COX-2 antibody be used to study the effects of inflammatory stimuli on macrophages?

Yes, the COX-2 antibody has been utilized in studies examining the role of COX-2 in macrophage responses to inflammatory stimuli. For instance, it was used to investigate how Yersinia pseudotuberculosis affects COX-2-mediated PGE2 biosynthesis in macrophages, highlighting its importance in the inflammatory response (Sheppe et al., 2021).

Is this antibody effective for detecting COX-2 in human gastric cancer cell lines?

The COX-2 antibody has been shown to effectively detect COX-2 expression in human gastric carcinoma epithelial cell lines, as demonstrated in research where it was used to assess the impact of herbal melanin on COX-2 expression and its downstream effects on PGE2 and IL-6 production (El-Obeid et al., 2023).

How does this antibody perform in Western blot applications?

The COX-2 antibody has been successfully employed in Western blot analyses to detect COX-2 protein levels in various studies, including those investigating the modulation of COX-2 expression in response to different treatments in macrophages and cancer cells (Wu et al., 2020; Zhao et al., 2019).

What is the specificity of this antibody for COX-2 in different species?

This COX-2 antibody is specific for detecting COX-2 in both human and mouse samples, with less than 1% cross-reactivity with recombinant human COX-1, making it suitable for comparative studies across these species (R&D Systems).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) CHI3L1 monoclonal antibody therapy mitigates cognitive impairment by inhibiting neuroinflammation through ERK and NF-κB pathway in Tg2576 miceFrontiers in Molecular NeuroscienceJanuary 7, 2026Hyeon Joo Ham, Seung Sik Park, Yong Sun Lee, Tae Hun Kim, Dong Ju Son, Ji-Hun Kim, Key-Hwan Lim, Hanseul Park, Hye Jin Lee, Jaesuk Yun, et al.ab32136, and ab183612, respectively), COX-2 and HAX1 (1:1000; Novus Biologicals, Inc., CO, USA; Cat No. AF4198 and NBP2-57945, respectively), ERK 1/2, p-ERK 1/2, JNK, p38, p-p38, IκBα, p-IκBα, and Myc-tag (1:1000;

(2) Interleukin-1 regulates myeloid cell trafficking and cerebral blood flow following intracerebral haemorrhageDisease Models & MechanismsOctober 1, 2025Jack Barrington, Jack Rivers-Auty, Patrick Strangward, Sabrina Tamburrano, Nikolett Lénárt, Tessa Swanton, Eloise Lemarchand, Adrian R. Parry-Jones, Ádám Dénes, David Brough, et al.protein 1 (2 µg ml−1, AF643, R&D Systems, RRID:AB_355499), goat-anti-cyclooxygenase-2 (0.5 µg ml−1, AF4198, R&D Systems, RRID:AB_2229909) and rabbit-anti-laminin (0.25 µg ml−1, ab11575, Abcam, RRID:AB_298179).

(3) Secretome of brain microvascular endothelial cells promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakageMolecular medicine (Cambridge, Mass.)August 26, 2024Loiola RA, Hachani J, Duban-Deweer S, Sevin E, Bugno P, Kowalska A, Rizzi E, Shimizu F, Kanda T, Mysiorek C, et al.Target,Reference,Provider COX-2,AF4198,R&D systems Phospho-VEGFR2,AF1766,R&D systems Phospho-AKT,MAB887,R&D systems pan-VEGFR2,sc6251,SantaCruz Non-phospho(active)-β

(4) Arachidonic acid inhibition of the NLRP3 inflammasome is a mechanism to explain the anti-inflammatory effects of fastingCell ReportsFebruary 27, 2024Milton Pereira, Jonathan Liang, Joy Edwards-Hicks, Allison M. Meadows, Christine Hinz, Sonia Liggi, Matthias Hepprich, Jonathan M. Mudry, Kim Han, Julian L. Griffin, et al.β-Actin (mouse) (AB3280, ABCAM) 1 in 2500; NLRP3 (rat) (MAB7578-SP, R&D Systems) 1 in 2000; COX2 (goat) (AF4198, R&D Systems). The secondary antibodies used were: anti-goat IgG-HRP (sc-2922, Santa Cruz) 1 in 5000;

(5) Human dental pulp stem cells modulate pro-inflammatory macrophages both through cell-to-cell contact and paracrine signalingFrontiers in immunologyJanuary 1, 2024Maccaferri M, Pisciotta A, Carnevale G, Salvarani C, Pignatti E.Thermo Fisher Scientific Inc., USA), IL-6 (dilution 1:1000, 12153, Cell Signaling Technology, USA), COX-2 (dilution 1:200, AF4198, Bio-Techne, USA). Membranes were rinsed, and then incubated with the appropriate secondary antibody

(6) Herbal melanin modulates PGE2 and IL-6 gastroprotective markers through COX-2 and TLR4 signaling in the gastric cancer cell line AGSBMC complementary medicine and therapiesSeptember 1, 2023El-Obeid A, Maashi Y, AlRoshody R, Alatar G, Aljudayi M, Al-Eidi H, AlGaith N, Khan AH, Hassib A, Matou-Nasri S.detection of COX-2, IL-6, TLR4, phospho-p65 NFκB, and total p65 NF-κB using goat anti-COX-2 antibody (#AF4198, R&D systems, Minneapolis, MN, USA), rabbit polyclonal anti-IL-6 antibody (#TA328217, OriGene Technologies

(7) Blocking Dectin-1 prevents colorectal tumorigenesis by suppressing prostaglandin E2 production in myeloid-derived suppressor cells and enhancing IL-22 binding protein expressionNature communicationsMarch 17, 2023Tang C, Sun H, Kadoki M, Han W, Ye X, Makusheva Y, Deng J, Feng B, Qiu D, Tan Y, et al.

(8) Combined anticancer therapy with imidazoacridinone analogue C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesisJournal of cellular and molecular medicineJuly 1, 2022Świtalska M, Filip-Psurska B, Milczarek M, Psurski M, Moszyńska A, Dąbrowska AM, Gawrońska M, Krzymiński K, Bagiński M, Bartoszewski R, et al.size‐sorting system (ProteinSimple). The evaluation of targeted proteins, Ang‐4 (MAB964; R&D Systems), COX‐2 (AF4198; R&D Systems), p53 (MAB1355; R&D Systems), ERK1/ERK2 (MAB1576; R&D Systems), phospho‐ERK1(T202/Y204)/ERK2

(9) Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakageStem cell research & therapyOctober 26, 2021Loiola RA, García-Gabilondo M, Grayston A, Bugno P, Kowalska A, Duban-Deweer S, Rizzi E, Hachani J, Sano Y, Shimizu F, et al.Target,Reference,Provider COX-2,AF4198,R&D Systems Phospho-VEGFR2,AF1766,R&D Systems Phospho-AKT,MAB887,R&D Systems pan-VEGFR2,sc6251,SantaCruz Non-phospho(active)-β

(10) Yersinia pseudotuberculosis YopJ Limits Macrophage Response by Downregulating COX-2-Mediated Biosynthesis of PGE2 in a MAPK/ERK-Dependent MannerMicrobiology spectrumSeptember 3, 2021Sheppe AEF, Santelices J, Czyz DM, Edelmann MJ.antibodies and enhanced chemiluminescence. The following antibodies were used in this study: COX-2 antibody AF4198 from R&D Systems (1:300 dilution), IL-1β antibody SC-7884 from Santa Cruz (1:1,000), β-actin antibody

(11) The inhibitory effects of Orengedokuto on inducible PGE2 production in BV-2 microglial cellsHeliyonAugust 1, 2021Iwata Y, Miyao M, Hirotsu A, Tatsumi K, Matsuyama T, Uetsuki N, Tanaka T.Cell Signaling), phospho-p38 MAPK (#9211; Cell Signaling), COX-1 (#ab695; abcam, Cambs, UK), COX-2 (#AF4198; R&D systems, MN, USA), cPGES (#15216-1-AP; Proteintech, IL, USA), phospho-cPLA2 (#2831; Cell Signaling),

(12) Macrophage phenotypic switch orchestrates the inflammation and repair/regeneration following acute pancreatitis injuryEBioMedicineAugust 1, 2020Wu J, Zhang L, Shi J, He R, Yang W, Habtezion A, Niu N, Lu P, Xue J.using a Foxp3 Transcription Factor Staining Buffer Set (eBioscience). PE-Ki67 (clone 16A8) and COX-2 (AF4198, R&D Systems) together with donkey anti-goat AF488 (Invitrogen) were used for intracellular staining.

(13) TET2 Regulates the Neuroinflammatory Response in MicrogliaCell reportsOctober 15, 2019Carrillo-Jimenez A, Deniz Ö, Niklison-Chirou MV, Ruiz R, Bezerra-Salomão K, Stratoulias V, Amouroux R, Yip PK, Vilalta A, Cheray M, et al.

(14) Response gene to complement 32 expression in macrophages augments paracrine stimulation-mediated colon cancer progressionCell death & diseaseOctober 10, 2019Zhao P, Wang B, Zhang Z, Zhang W, Liu Y.was obtained from Sigma (P1585). Anti-COX-2 and anti-CD68 antibodies were purchased from R&D Systems (AF4198 and MAB20401). Rabbit polyclonal anti-RGC-32 antibody was purchased from Biorbyt (orb2372). Anti-smad2

(15) Multi-Staged Regulation of Lipid Signaling Mediators during Myogenesis by COX-1/2 PathwaysInternational journal of molecular sciencesSeptember 4, 2019Mo C, Wang Z, Bonewald L, Brotto M.

(16) Increased Toxoplasma gondii Intracellular Proliferation in Human Extravillous Trophoblast Cells (HTR8/SVneo Line) Is Sequentially Triggered by MIF, ERK1/2, and COX-2Frontiers in microbiologyJanuary 1, 2019Milian ICB, Silva RJ, Manzan-Martins C, Barbosa BF, Guirelli PM, Ribeiro M, de Oliveira Gomes A, Ietta F, Mineo JR, Silva Franco P, et al.

(17) Stromal versus tumoral inflammation differentially contribute to metastasis and poor survival in laryngeal squamous cell carcinomaOncotargetFebruary 2, 2018Höing B, Kanaan O, Altenhoff P, Petri R, Thangavelu K, Schlüter A, Lang S, Bankfalvi A, Brandau S.

(18) Interleukin-1β induced Stress Granules Sequester COX-2 mRNA and Regulates its Stability and Translation in Human OA ChondrocytesScientific reportsJune 8, 2016Ansari MY, Haqqi TM.phospho-eIF2α (#9721) were from Cell Signaling Technology (Beverly, MA, USA) and anti-COX-2 antibody (AF4198) was from R&D Systems. Horseradish peroxidase conjugated appropriate secondary antibodies were from

(19) Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in miceScience advancesJanuary 1, 0001"Liu J, Lane S, Lall R, Russo M, Farrell L, Debreli Coskun M, Curtin C, Araujo-Gutierrez R, Scherrer-Crosbie M, Trachtenberg BH, et al."AF7007), HO-1 (1:1000; Abcam, ab13248), Nrf2 (1:1000; Abcam, ab137550), Ptgs2/COX2 (1:1000; R&D System, AF4198), GPX4 (1:2000; R&D System, MAB5457), cleaved caspase-3 (1:500; Cell Signaling Technology, 9654S), human

(20) Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel DiseaseCellJanuary 1, 0001"Kinchen J, Chen HH, Parikh K, Antanaviciute A, Jagielowicz M, Fawkner-Corbett D, Ashley N, Cubitt L, Mellado-Gomez E, Attar M, et al."LIGHT/TNFSF14,R and D Systems,Cat#AF664; RRID: AB_355512 Goat polyclonal anti-human/mouse COX-2,Bio-Techne,Cat#AF4198; RRID: AB_2229909 Goat polyclonal anti-human Coagulation Factor III/Tissue Factor,Bio-Techne,Cat#AF2339;

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COX-2 Antibody

COX-2 Antibody

COX-2 Antibody

COX-2 Antibody

COX-2 Antibody

COX-2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (20)

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COX-2 Antibody from R&D Systems, a Bio-Techne Brand

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