Human Matriptase/ST14 Catalytic Domain Antibody AF3946 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Matriptase/ST14 Catalytic Domain Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF3946
NCBI Gene AliasesMT-SP1, HAI, PRSS14, CAP3, TADG15, SNC19, TMPRSS14, ARCI11, MTSP1
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
ReactivityHuman,Insect - Drosophila,Mouse
IsotypeIgG
HostSheep
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetMatriptase/ST14
NCBI Full Gene NameST14 transmembrane serine protease matriptase
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Matriptase/ST14 Catalytic Domain, Val615-Val855, Accession # Q9Y5Y6
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used to study the role of matriptase in cancer progression?

Yes, the Matriptase/ST14 Catalytic Domain Antibody has been utilized in studies investigating its role in cancer, particularly in squamous cell carcinomas. Research has shown that matriptase promotes multi-stage carcinogenesis through activation of signaling pathways that contribute to tumor progression and inflammatory responses ().

Is this antibody effective for immunohistochemistry applications?

The Matriptase/ST14 Catalytic Domain Antibody has been successfully used in immunohistochemistry, as demonstrated in studies where it was employed to analyze tissue samples from transgenic mice. The antibody was used to detect matriptase expression in various tissues, confirming its utility in this application ().

What species can this antibody detect, and is it suitable for human studies?

This antibody is reactive with multiple species, including humans, mice, and Drosophila. It has been shown to effectively detect human matriptase in various experimental setups, making it suitable for studies involving human tissues (, ).

Has this antibody been cited in any significant research studies?

Yes, the Matriptase/ST14 Catalytic Domain Antibody has been cited in 17 scientific articles, indicating its relevance and application in various research contexts, including studies on viral activation and cancer biology (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) From Dysplasia to Carcinoma: Expression Patterns of Dermokine, Matriptase, and Tryptase in OPMDOral DiseasesJuly 24, 2025Lara Maria Alencar Ramos Innocentini, Mateus Gonçalves Miranda, Carol Kobori da Fonseca, Pedro Franco Ferreira, Leandro Dorigan de Macedo, Graziela Cavalcanti, Luiz Carlos Conti de Freitas, Ana Carolina Fragoso Motta, Alan Grupioni Lourenço, Katiuchia Uzzun Salesproduced in rabbit, Elabscience, Houston, Texas, USA); Anti‐matriptase (concentration: 20 μg/mL—Cat.: AF3946, produced in sheep, R&D Systems, Minneapolis, MN, USA); Anti‐tryptase (concentration: 20 μg/mL—Cat.:

(2) Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transportLife science allianceMarch 1, 2024Rickman OJ, Guignard E, Chabanon T, Bertoldi G, Auberson M, Hummler E.Invitrogen Claudin-7,Rabbit,1:250,1:125,#34-9100; Invitrogen Tubulin,Mouse,"1:2,000","1:1,000",#T5168; Sigma-Aldrich St14,Rabbit,1:500,,#AF3946; R&D Systems Prss8,Sheep,1:500,,#15527-1-AP; ProteinTech ZO-1,Rabbit,1:500,,#61-7300; Zymed Occludin,Rabbit,1:500,,#71-1500;

(3) Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and CAP3/St14 Affect ENaC Subunit Abundances but Not Its ActivityCellsSeptember 23, 2023Ehret E, Stroh S, Auberson M, Ino F, Jäger Y, Maillard M, Szabo R, Bugge TH, Frateschi S, Hummler E.NHE3, NKCC2 [24,25], γENaC (StressMarq, Biosciences, Victoria, Canasa) [26,27], CAP3/St14 (R&D systems AF3946, Minneapolis, MN, USA), BK/Maxi-Ki, Kca1.1 (APC-151, Alomone labs, Jerusalem, Israel). RomK/Kir1.1 was

(4) Exosome-Mediated Activation of the Prostasin-Matriptase Serine Protease Cascade in B Lymphoma CellsCancersJuly 28, 2023Chen LM, Chai KX.Santa Cruz Biotechnology, Inc., Dallas, TX, USA, or A300-221A, polyclonal, Bethyl Laboratories, Montgomery, TX, USA, or AF3946, polyclonal, R&D Systems, Inc., Minneapolis, MN, USA); HAI-2 (AF1106, R&D Systems, Inc.); and GAPDH

(5) Kallikrein 5 Inhibition by the Lympho-Epithelial Kazal-Type Related Inhibitor Hinders Matriptase-Dependent CarcinogenesisCancersAugust 31, 2021da Silva EZM, Fraga-Silva TFC, Yuan Y, Alves MG, Publio GA, da Fonseca CK, Kodama MH, Vieira GV, Candido MF, Innocentini LMAR, et al.µg/mL; clone HPA009067, Merck, Darmstadt, Germany), anti-Matriptase-ST14 (1:400 dilution, Catalog# AF3946, R&D Systems, Minneapolis, MN, USA) and anti-mouse Kallikrein 5 (1:800 dilution; Catalog# MAB7236, R&D Systems

(6) Zymogen-locked mutant prostasin (Prss8) leads to incomplete proteolytic activation of the epithelial sodium channel (ENaC) and severely compromises triamterene tolerance in miceActa physiologica (Oxford, England)May 1, 2021Essigke D, Ilyaskin AV, Wörn M, Bohnert BN, Xiao M, Daniel C, Amann K, Birkenfeld AL, Szabo R, Bugge TH, et al.(SPC-402, Stressmarq), NKCC2 (SPC-401, Stressmarq), prostasin (15527-1-AP, proteintech) and matriptase (AF3946, R&D systems). Statistical analysis Data are provided as means with SEM. Data were tested for normality

(7) Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated ClaudinsCellsApril 21, 2020Wu CJ, Lu M, Feng X, Nakato G, Udey MC.1027 3 of 12 Polyclonal anti-TROP2, polyclonal goat anti-human HAI-1 (AF1048), sheep anti-matriptase (AF3946), goat anti-mouse HAI-1 (AF1141), and goat anti-mouse HAI-2 (AF1107) Abs were purchased from R & D Systems

(8) Conserved function of the matriptase-prostasin proteolytic cascade during epithelial morphogenesisPLoS geneticsJanuary 1, 2019Drees L, Königsmann T, Jaspers MHJ, Pflanz R, Riedel D, Schuh R.

(9) Inflammatory cytokines down-regulate the barrier-protective prostasin-matriptase proteolytic cascade early in experimental colitisThe Journal of biological chemistryJune 30, 2017Buzza MS, Johnson TA, Conway GD, Martin EW, Mukhopadhyay S, Shea-Donohue T, Antalis TM.

(10) Matriptase zymogen supports epithelial development, homeostasis and regenerationBMC biologyJune 1, 2017Friis S, Tadeo D, Le-Gall SM, Jürgensen HJ, Sales KU, Camerer E, Bugge TH.overnight at 4 °C. Antibodies used for mouse lysates were sheep anti-human matriptase (catalogue no. AF3946, R&D Systems) and mouse anti-human prostasin (catalogue no. 612173, BD Transduction Laboratories, San

(11) Matriptase-mediated cleavage of EpCAM destabilizes claudins and dysregulates intestinal epithelial homeostasisThe Journal of clinical investigationFebruary 1, 2017Wu CJ, Feng X, Lu M, Morimura S, Udey MC.Biosciences, and sheep Inhibition of protein expression with siRNAs. Double-stranded anti-matriptase (AF3946) was from R&D Systems. Polyclonal anti– RNA oligonucleotides containing matriptase siRNAs (siMatriptase-1, 632 jci.org Volume

(12) Distinct Developmental Functions of Prostasin (CAP1/PRSS8) Zymogen and Activated ProstasinThe Journal of biological chemistryFebruary 5, 2016Friis S, Madsen DH, Bugge TH.

(13) Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumorsOncogeneAugust 27, 2015Sales KU, Friis S, Abusleme L, Moutsopoulos NM, Bugge TH.Tween 20 (TBS-T) for 1 h at room temperature. The membrane was probed with anti-matriptase antibody (AF3946, R&D Systems) diluted in 1% nonfat dry milk in TBS-T overnight at 4 °C. The next day, the membrane was

(14) Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signallingNature communicationsApril 15, 2015Zoratti GL, Tanabe LM, Varela FA, Murray AS, Bergum C, Colombo É, Lang JE, Molinolo AA, Leduc R, Marsault E, et al.

(15) Regulation of feto-maternal barrier by matriptase- and PAR-2-mediated signaling is required for placental morphogenesis and mouse embryonic survivalPLoS geneticsJuly 1, 2014Szabo R, Peters DE, Kosa P, Camerer E, Bugge TH.

(16) Matriptase proteolytically activates influenza virus and promotes multicycle replication in the human airway epitheliumJournal of virologyApril 1, 2013Beaulieu A, Gravel É, Cloutier A, Marois I, Colombo É, Désilets A, Verreault C, Leduc R, Marsault É, Richter MV.with 1% PFA (20 min). Next, coverslips were incubated with the sheep anti-matriptase antibody (1:25) (AF3946; R&D Systems) and chicken anti-early endosomal antigen 1 (EEA-1) antibody (1:200) (GTX82138; GeneTex,

(17) Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activationPLoS geneticsJanuary 1, 2012Szabo R, Uzzun Sales K, Kosa P, Shylo NA, Godiksen S, Hansen KK, Friis S, Gutkind JS, Vogel LK, Hummler E, et al.

(18) c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptaseOncogeneApril 28, 2011Szabo R, Rasmussen AL, Moyer AB, Kosa P, Schafer JM, Molinolo AA, Gutkind JS, Bugge TH.

(19) Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiationThe Journal of biological chemistryFebruary 18, 2011Fang JD, Chou HC, Tung HH, Huang PY, Lee SL.

(20) Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndromeNature geneticsAugust 1, 2010Sales KU, Masedunskas A, Bey AL, Rasmussen AL, Weigert R, List K, Szabo R, Overbeek PA, Bugge TH.

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Matriptase/ST14 Catalytic Domain Antibody

Matriptase/ST14 Catalytic Domain Antibody

Human Matriptase/ST14 Catalytic Domain Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (21)

Reviews

Human Matriptase/ST14 Catalytic Domain Antibody from R&D Systems, a Bio-Techne Brand

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