Fig 2: Effect of IL17RA knockdown and centrinone on squamous skin cancer cells. (A) Endogenous IL17RA of HaCaT and A431 was knocked down using small interference RNA of IL17RA. (B,C) Colony formation assay was conducted to determine the effect of IL17RA knockdown and inhibition of PLK4 on the proliferation of normal skin cell line HaCaT and squamous skin cancer cell line A431. A two-tailed Student’s t test was applied to analyze statistical significance. Error bar represents mean ± standard deviation (SD); ns—no significance; ** —p < 0.01.

Fig 3: Expression pattern of IL17 and PLK family members in skin cancer and autoimmune diseases. (A,B) Original data of GSE109248 were analyzed using the OmicSoft platform. The heatmap shows the expression patterns of PLK family members among normal skin, cutaneous lupus erythematosus, and psoriasis. (C) The GEO2R tool was applied to differentially determine the expressed genes (p value = 0.01) between psoriasis and normal skin. (D) The heatmap shows expression patterns of IL17 family members and PLK family members among normal skin, actinic keratosis (AK), and squamous cell carcinoma (SCC) of skin. Data were downloaded from GDS2200 database. (E) The volcano plot demonstrates significant genes between SCC and normal skin. Data were downloaded from GSE2503. (F) Immunohistochemical staining of human tissues from The Human Protein Atlas (HPA, https://www.proteinatlas.org/, accessed on 12 July 2022) shows expression patterns of IL17RA, IL17RC, and PLK4 between normal skin and SCC.

Fig 4: Knockout of Il17rc alleviates skin lesions induced by imiquimod. (A) Diagram of establishing the imiquimod-induced psoriasis mouse model and skin lesions of WT C57BL/6J, Il17ra(T779A)-knockin, and Il17rc-knockout strains. (B) Representatives of hematoxylin and eosin stain of skin lesions. Scale bar represents 100 µm. (C) To analyze the statistical significance of the thickness of epidermis, a two-tailed Student’s t test was applied. Error bar represents mean ± SD. **** indicates p < 0.0001. The numbers of male and female mice in each group are indicated in the panel.