Phospho-VEGFR1/Flt-1 (Y1213) Antibody AF4170 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPhospho-VEGFR1/Flt-1 (Y1213) Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $429.00Supplier Page View Company Product Page
Catalog NumberAF4170
ApplicationsImmunohistochemistry,Western Blot,Immunocytochemistry,Cell Culture
ReactivityHuman,Mouse
IsotypeIgG
HostRabbit
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetVEGFR1/Flt-1
ClonalityPolyclonal
Modificationsp tyr1213
ImmunogenPhosphopeptide containing human VEGFR1/Flt-1 Y1213 site
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namefms related receptor tyrosine kinase 1
NCBI Gene AliasesFLT, FLT-1, VEGFR-1, VEGFR1

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human VEGFR1/Flt-1 when phosphorylated at Y1213.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the role of VEGFR1 in myocardial ischemia/reperfusion injury?

Yes, the Phospho-VEGFR1/Flt-1 (Y1213) Antibody has been utilized in studies investigating myocardial ischemia/reperfusion injury. For instance, in a study by Zhang et al. (2018), the antibody was employed in Western blot analysis to assess the activation of VEGFR1, which was shown to protect cardiac tissue from oxidative stress and apoptosis during ischemic events.

Is this antibody effective for detecting phosphorylated VEGFR1 in human samples?

The Phospho-VEGFR1/Flt-1 (Y1213) Antibody is specifically designed to detect human VEGFR1 when phosphorylated at the Y1213 site. This specificity allows for accurate analysis of VEGFR1 activation in human samples, which is crucial for understanding its role in various biological processes and diseases.

What applications can I use this antibody for in my research?

This antibody is suitable for use in Western blotting and immunohistochemistry. It has been shown to work effectively in these applications, allowing researchers to analyze protein expression and localization in various experimental setups.

Are there any studies that demonstrate the functional relevance of VEGFR1 phosphorylation in cancer?

Yes, a study by Blanchard et al. (2018) explored the regulatory link between angiogenesis and tumor suppressor gene expression, highlighting the role of VEGFR1 in angiogenesis signaling. The findings suggest that modulation of VEGFR1 activity can influence tumor biology, making the Phospho-VEGFR1/Flt-1 (Y1213) Antibody a valuable tool for investigating these pathways in cancer research.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

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(3) lncRNA PVT1 Promotes Tumorigenesis of Colorectal Cancer by Stabilizing miR-16-5p and Interacting with the VEGFA/VEGFR1/AKT AxisMolecular therapy. Nucleic acidsJune 5, 2020Wu H, Wei M, Jiang X, Tan J, Xu W, Fan X, Zhang R, Ding C, Zhao F, Shao X, et al.USA), p-AKT (66444-1-lg, Proteintech, USA), VEGFA (ab52917, Abcam, Cambridge, MA, USA), or p-VEGFR1 (AF4170, R&D Systems, USA) antibody for 3 h at room temperature. The cells were washed using PBS followed by

(4) Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon CancerCancersFebruary 8, 2019Blanchard TG, Czinn SJ, Banerjee V, Sharda N, Bafford AC, Mubariz F, Morozov D, Passaniti A, Ahmed H, Banerjee A.(Cell Signaling, #9275), total Akt (Cell Signaling, #9272), p-VEGFR1/Flt-1 (Y1213) (R and D Systems, #AF4170), p-VEGFR2 /KDR/Flk-1 (Y1214) (R and D Systems, Minneapolis, MN, USA, #AF1766), total VEGFR1 (R&D systems,

(5) Treatment with placental growth factor attenuates myocardial ischemia/reperfusion injuryPloS oneJanuary 1, 2018Zhang Y, Cao C, Xin J, Lv P, Chen D, Li S, Yang H, Chen C, Liu B, Li Q.membranes. Standard Western blots analysis were performed using antibodies against p-VEGFR1 (R&D Systems, AF4170), VEGFR1 (Abcam, ab32152), p-AKT (Abcam, ab81283), AKT (Abcam, ab32505), p-GSK3β (Abcam, ab75745), GSK3β

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(8) The anti-vascular endothelial growth factor receptor-1 monoclonal antibody D16F7 inhibits invasiveness of human glioblastoma and glioblastoma stem cellsJournal of experimental & clinical cancer research : CRAugust 10, 2017Atzori MG, Tentori L, Ruffini F, Ceci C, Lisi L, Bonanno E, Scimeca M, Eskilsson E, Daubon T, Miletic H, et al.

(9) BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor GrowthClinical cancer research : an official journal of the American Association for Cancer ResearchApril 1, 2015Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, et al.

(10) Deletion of placental growth factor prevents diabetic retinopathy and is associated with Akt activation and HIF1α-VEGF pathway inhibitionDiabetesJanuary 1, 2015Huang H, He J, Johnson D, Wei Y, Liu Y, Wang S, Lutty GA, Duh EJ, Semba RD.

(11) Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophagesJournal of immunology (Baltimore, Md. : 1950)April 15, 2012Meda C, Molla F, De Pizzol M, Regano D, Maione F, Capano S, Locati M, Mantovani A, Latini R, Bussolino F, et al.

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(13) Density of human bone marrow stromal cells regulates commitment to vascular lineagesStem cell researchMay 1, 2011Whyte JL, Ball SG, Shuttleworth CA, Brennan K, Kielty CM.immunofluorescence analysis were goat anti-human VEGFR1 (AF321), rabbit anti-human phospho-VEGFR1 (Y1213) (AF4170), and goat anti-human hairy enhancer of split-1 (HES-1) (AF3317), all from R&D Systems. Mouse anti-human

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