Fibroblast Activation Protein alpha/FAP Antibody AF3715 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemFibroblast Activation Protein alpha/FAP Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF3715
NCBI Gene AliasesSIMP, DPPIV, FAPA, FAPalpha
ApplicationsPhotoimmunotherapy,Western Blot,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry, Dual RNAscope ISH-IHC Compatible
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Transgenic Mouse,Xenograft
TargetFibroblast Activation Protein alpha/FAP
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
Quantity100 ug (also 25 ug)
HostSheep
NCBI Full Gene Namefibroblast activation protein alpha
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP, Leu26-Asp760, Accession # Q12884
Antibody TypePrimary Antibodies
ValidationKnockout Validated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
PurityAntigen Affinity-purified
ConcentrationLYOPH
ClonalityPolyclonal

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for studying fibroblast activation protein alpha in human cancer tissues?

Yes, the Fibroblast Activation Protein alpha/FAP Antibody has been shown to effectively detect FAP in human cancer tissues through immunohistochemistry, as demonstrated in studies involving breast cancer and esophageal squamous cell carcinoma tissues (, ).

Is this antibody suitable for use in flow cytometry applications?

The Fibroblast Activation Protein alpha/FAP Antibody has been utilized in flow cytometry, where it was employed to analyze FAP expression on various cell populations, including tumor-associated macrophages ().

How has this antibody been validated in experimental settings?

The antibody has been validated through knockout studies and has been cited in multiple publications for its effectiveness in Western blotting and immunofluorescence applications, confirming its specificity for FAP in various experimental contexts (, ).

What role does fibroblast activation protein alpha play in cancer progression, and how does this antibody contribute to that research?

Fibroblast activation protein alpha is implicated in tumorigenicity and is often associated with cancer-associated fibroblasts, which promote tumor progression. The antibody can be used to investigate FAP's role in signaling pathways and its interaction with other proteins in cancer cells, as shown in studies focusing on ovarian and esophageal cancers (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Emergence of a mixed CAF population by FAP-CD3 T-cell engager limits therapeutic efficacyJ Immunother CancerJuly 3, 2026Robert J Norgard, Joshua R Tagore, Pratha Budhani, Sai Charan Penikalapati, Priyanka Gupta, Dongmei Liu, Jessica N Egan, Sarah F Finnegan, Zhuxuan Li, Brianna Flynn, et al.phosphate buffered saline (PBS) to remove excess TcE and stained with FAP alpha antibody (R&D Systems #AF3715, 1:20 dilution) for 30 min. Cells were stained with a secondary Goat-anti-sheep PE (R&D Systems #F0126,

(2) Deep phenotyping of skin tissue remodeling in patients with systemic sclerosis treated with CD19-CAR T cellsNat CommunMay 23, 2026Aleix Rius Rigau, Meilin Xu, Ziyuan Liu, Sara Chenguiti Fakhouri, Janina Auth, Panagiotis Garantziotis, Andrea Zoli, Manoj Kumar Selvaraju, Maria Gabriella Raimondo, Carlo Tur, et al.CD31 (1:200, #AF3628; R&D Systems, Minneapolis, USA), Fibroblast activation protein α (FAP) (1:200, #AF3715; R&D Systems, Minneapolis, USA), prolyl-4-hydroxylase β (P4Hβ) (1:200, AF4236, R&D Systems, Minneapolis,

(3) Bone Marrow Stromal Cell Antigen 2 Promotes Esophageal Squamous Cell Carcinoma Progression by Mediating the Cell-Cell Interaction between Cancer Cells and Cancer-Associated FibroblastsThe American Journal of PathologyMay 1, 2026Rikuya Torigoe, Yu-ichiro Koma, Naozane Nomura, Hiroki Yokoo, Masaki Omori, Takashi Nakanishi, Shoji Miyako, Takaaki Nakanishi, Takayuki Kodama, Manabu Shigeoka, et al.(1:150; number 2929; Cell Signaling Technology), and fibroblast activation protein (FAP; 1:300; number AF3715; R&D Systems, Minneapolis, MM). After washing, the cells were stained with DAPI (1:1000; number 340-07971;

(4) Assessment of the Diagnostic Value of [68Ga]Ga-FAPI-04 and [18F]FDG PET in a PHMG-p-Induced Pulmonary Fibrosis Murine ModelDiagnostics (Basel)December 19, 2025So Young Kim, Jun Young Park, Ye Lim Cho, Won Jun Kangincubated with the sheep polyclonal anti-human fibroblast activation protein alpha (FAP) antibody (Cat# AF3715; 1:400, R&D, Minneapolis, MN, USA) at 4 °C overnight and horseradish peroxidase (HRP)-conjugated secondary

(5) BGN Secreted by Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression via Activation of TLR4-Mediated Erk and NF-κB Signaling PathwaysInternational journal of molecular sciencesDecember 13, 2025Yokoo H, Koma YI, Nomura N, Torigoe R, Omori M, Nakanishi T, Miyako S, Nakanishi T, Kodama T, Shigeoka M, et al.(tNF-κB; #8242, CST), mouse TLR4 (#sc-293072, Santa Cruz Biotechmology, Dallas, TX, USA), sheep FAP (#AF3715, R&D Systems), rabbit αSMA (#ab5694, Abcam, Cambridge, UK), rabbit IL-6 (#ab6672, Abcam), mouse CD163

(6) Senolytic-sensitive p16(Ink4a)+ fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticityCell stem cellDecember 4, 2025Lee JY, Reyes N, Woo SH, Allen NC, Kadota T, Lechner A, Biswas R, Goel S, Stratton F, Kuang C, et al.p16INK4a (1:200, Santa Cruz, sc-56330), APOE (1:200, Invitrogen, 701241), FAP (1:200, R&D systems, AF3715). Images were captured using Zeiss Imager M1 or Leica Stellaris 5. For organoids, the Matrigel containing

(7) Fibroblast Activation Protein (FAP)(+) cancer-associated fibroblasts induce macrophage M2-like polarization via the Fibronectin 1-Integrin α5β1 axis in breast cancerOncogeneJuly 1, 2025Chen W, Jiang M, Zou X, Chen Z, Shen L, Hu J, Kong M, Huang J, Ni C, Xia W.

(8) Disturbed spatial WNT activation - a potential driver of the reticularized skin phenotype in Systemic Sclerosis (SSc)Arthritis & RheumatologyMarch 11, 2025Sara Chenguiti Chenguiti Fakhouri, Honglin Zhu, Yi‐Nan Li, Moritz Ronicke, Aleix Rius Rigau, Clara Dees, Laura Konstantinidis, Ralf Schmid, Alexandru‐Emil Matei, Markus Eckstein, et al.R&D Systems), vimentin (1:1000, NBP1‐97515; Novus Biologicals), Fibroblast activation protein α (FAP) (1:200, #AF3715; R&D Systems), CD90/Thy1 (1:250, #ab133350; abcam). Conjugated secondary antibodies (1:200, Alexa Fluor,

(9) Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac RepairInternational journal of nanomedicineJanuary 1, 2025Kang JY, Mun D, Park M, Yoo G, Kim H, Yun N, Joung B.cryosectioned 24 h post-injection and subjected to immunofluorescence staining with anti-FAP antibody (AF3715, R&D Systems, Minneapolis, MN, USA) and 4′,6-diamidino-2-phenylindole (DAPI; 62248, Thermo Fisher Scientific).

(10) AREG Upregulation in Cancer Cells via Direct Interaction with Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression Through EGFR-Erk/p38 MAPK SignalingCellsOctober 19, 2024Takashi Nakanishi, Yu-ichiro Koma, Shoji Miyako, Rikuya Torigoe, Hiroki Yokoo, Masaki Omori, Keitaro Yamanaka, Nobuaki Ishihara, Shuichi Tsukamoto, Takayuki Kodama, et al.Receptor (Tyr1068) antibody (#2234, CST), rabbit EGF Receptor antibody (#4267, CST), sheep FAP antibody (#AF3715, R&D Systems; Minneapolis, MN, USA), rabbit IL-6 antibody (#ab6672, Abcam, Cambridge, UK), rabbit αSMA

(11) The multifaceted role of the stroma in the healthy prostate and prostate cancerJournal of translational medicineSeptember 5, 2024Di Carlo E, Sorrentino C.Immunostaining with anti-FAP antibody (#AF3715; R&D Systems, Minneapolis, MN, USA) highlights the absence of CAFs in the stroma of normal prostate

(12) Expression of fibroblast activation protein-α in human deep vein thrombosisThrombosis researchSeptember 1, 2024Oguri N, Gi T, Nakamura E, Furukoji E, Goto H, Maekawa K, Tsuji AB, Nishii R, Aman M, Moriguchi-Goto S, et al.

(13) Senescent fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticitybioRxiv : the preprint server for biologyJuly 30, 2024Lee JY, Reyes N, Woo SH, Goel S, Stratton F, Kuang C, Mansfield AS, LaFave LM, Peng T.

(14) Cancer-associated fibroblast phenotypes are associated with patient outcome in non-small cell lung cancerCancer cellMarch 11, 2024Cords L, Engler S, Haberecker M, Rüschoff JH, Moch H, de Souza N, Bodenmiller B.RRID: AB_10807552 Sheep polyclonal anti-FAP; Clone (polyclonal_FAP); Lot(ZKW0619101),R&D Systems,Cat#AF3715; RRID: AB_2102369 Mouse monoclonal anti-CD68; Clone (KP1); Lot(2162103),eBioscience,Cat#14-0688-82;

(15) Obesity defined molecular endotypes in the synovium of patients with osteoarthritis provides a rationale for therapeutic targeting of fibroblast subsetsClinical and translational medicineApril 1, 2023Wijesinghe SN, Badoume A, Nanus DE, Sharma-Oates A, Farah H, Certo M, Alnajjar F, Davis ET, Mauro C, Lindsay MA, et al.NDS/.1% Tween‐20/PBS. The following primary antibodies were incubated at 4°C overnight: anti‐FAPα (AF3715, R&D Systems), anti‐CXCL12 (MA5‐23759, Thermo Fisher Scientific), anti‐THBD (hpa002982, Atlas Antibodies),

(16) FAP is critical for ovarian cancer cell survival by sustaining NF-κB activation through recruitment of PRKDC in lipid raftsCancer gene therapyApril 1, 2023Li B, Ding Z, Calbay O, Li Y, Li T, Jin L, Huang S.mass spectrometry, samples were precipitated with anti-FAP polyclonal antibody (R&D systems, Cat # AF3715) and then sent to UF ICBR for analysis on TSQ Altis™ Triple Quadrupole Mass Spectrometer (Thermo Fisher

(17) Engineering edgeless human skin with enhanced biomechanical propertiesScience advancesJanuary 27, 2023Pappalardo A, Alvarez Cespedes D, Fang S, Herschman AR, Jeon EY, Myers KM, Kysar JW, Abaci HE.

(18) Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progressionBone researchJanuary 2, 2023Fan A, Wu G, Wang J, Lu L, Wang J, Wei H, Sun Y, Xu Y, Mo C, Zhang X, et al.blocking buffer (PBS with 5% horse serum) for 1 h at room temperature and then stained with anti-FAP (R&D, AF3715, 1:500), anti-OLN/CLEC11A (R&D, BAF1904, 1:500), anti-Oln/Clec11a (R&D systems, AF3729, 1:500), anti-beta

(19) Evaluating near-infrared photoimmunotherapy for targeting fibroblast activation protein-α expressing cells in vitro and in vivoCancer scienceJanuary 1, 2023Jin J, Barnett JD, Krishnamachary B, Mironchik Y, Luo CK, Kobayashi H, Bhujwalla ZM.soluble phthalocyanine dye IR700 was obtained from Li‐Cor Bioscience. Anti‐FAP‐α polyclonal sheep Ab, AF3715, monoclonal mouse Ab MAB3715, and monoclonal rat Ab MAB9729 were purchased from R&D Systems. Anti‐FAP‐α

(20) Functional roles of FAP-α in metabolism, migration and invasion of human cancer cellsFrontiers in oncologyJanuary 1, 2023Mori N, Jin J, Krishnamachary B, Mironchik Y, Wildes F, Vesuna F, Barnett JD, Bhujwalla ZM.Tween) or 5% BSA-TBST, the membrane was separately probed with antibodies against FAP-α (R&D Systems Inc AF3715. Minneapolis, MN, USA), choline kinase-alpha (Chk-α) (Proteintech Group, Chicago custom-made, IL, USA),

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