TCF4/TCF7L2 (C48H11) Rabbit mAb 2569S from Cell Signaling Technology

Product Specs
ItemTCF4/TCF7L2 (C48H11) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number2569S
Quantity100 µl
ApplicationsW, W-S, IP, ChIP, ChIP-seq, C&R, C&T
ReactivityH
TargetTCF4/TCF7L2
HostRabbit
NCBI Gene AliasesTCF-4, TCF4
ClonalityMonoclonal
NCBI Full Gene Nametranscription factor 7 like 2
Research AreaDevelopmental Biology
CloneC48H11
Molecular Weight58, 79

Questions and Answers

Can this antibody be used to study the Wnt/β-catenin signaling pathway in colorectal cancer?

Yes, the TCF4/TCF7L2 (C48H11) Rabbit mAb has been shown to be effective in western blotting for detecting total TCF4 protein levels, which is a key component of the Wnt/β-catenin signaling pathway. Studies have demonstrated that TCF4 interacts with β-catenin to regulate gene expression in colorectal cancer cells, highlighting its relevance in this context ().

Is this antibody suitable for chromatin immunoprecipitation assays?

The TCF4/TCF7L2 (C48H11) Rabbit mAb has been utilized in chromatin immunoprecipitation (ChIP) assays, as indicated in various studies. It has been employed to investigate the binding of TCF4 to target gene promoters, which is crucial for understanding its role in transcriptional regulation within the Wnt signaling pathway ().

What applications has this antibody been cited in?

The TCF4/TCF7L2 (C48H11) Rabbit mAb has been cited in multiple applications, including western blotting, immunoprecipitation, chromatin immunoprecipitation, and ChIP-seq. Its versatility makes it suitable for various experimental setups aimed at studying TCF4/TCF7L2 protein dynamics and function in human cells ().

Does this antibody have any documented effects on cancer cell behavior?

Yes, the TCF4/TCF7L2 (C48H11) Rabbit mAb has been referenced in studies that explore its role in cancer cell behavior, particularly in relation to epithelial-mesenchymal transition (EMT) and drug resistance mechanisms in colorectal cancer. For instance, it has been shown that TCF4 can influence the expression of microRNAs that promote EMT, thereby affecting cancer cell migration and invasion ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) A Double-Negative Prostate Cancer Subtype is Vulnerable to SWI/SNF-Targeting Degrader MoleculesCancer ResearchApril 2, 2026Phillip Thienger, Irene Paassen, Xiaosai Yao, Philip D. Rubin, Marika Lehner, Nicholas Lillis, Andrej Benjak, Sagar R. Shah, Alden King-Yung. Leung, Simone de Brot, et al.Research Unit platform, Bern (RRID: SCR_027566). The TCF7L2 antibody (Cell Signaling Technology, cat #2569, RRID: AB_2199816) was used for staining. The intensity of nuclear immunostaining was evaluated on whole

(2) Single-cell lung eQTL dataset of Asian never-smokers highlights the roles of alveolar cells in lung cancer etiologybioRxivMarch 31, 2026Thong Luong, Jinhu Yin, Bolun Li, Ju Hye Shin, Elelta Sisay, Sama Mikhail, Fei Qin, Samuel Anyaso-Samuel, Alexander Kane, Alyxandra Golden, et al.(xCELLigence). TCF7L2 knockdown was confirmed by western blotting for TCF7L2 (rabbit monoclonal, Cell Signaling #2569S, 1:1000) and GAPDH (mouse monoclonal, Santa Cruz Biotechnology #47724, 1:500). For xCELLigence, cells

(3) ROME, an ancient gene with a novel function in vertebrates, is a key modulator of embryonal development and cancer metastasisCancer Research CommunicationsMarch 1, 2026Anna Molotkova, Emre Deniz, Matthew Swift, Eric Glasgow, Junfeng Ma, Jeffrey A. Petro, Isabel Frye, Metin Ozdemirli, Dusan Pesic, Gerard P. Ahern, et al.Cell Signaling Technology, #8480, RRID: AB_11127855), anti-TCF4/-TCF7L2 (1:1,000 dilution; Cell Signaling Technology, #2569, RRID: AB_2199816), anti–V5-tag (1:1,000 dilution; Cell Signaling Technology, #13202, RRID: AB_2687461),

(4) Injury-transduced SerpinOLs modulate neuroinflammation and glial activation in the diseased and non-diseased CNSbioRxivJanuary 31, 2026Yan Wang, Meina Zhu, Joohyun Park, Hyun Kyu Kim, Xiong Ge, Xi Chen, Bo Chen, Shinghua Ding, Wensheng Lin, Fuzheng GuoRRID: AB_213434), Rabbit monoclonal anti-TCF4/TCF7L2 (C48H11) (1:100, Cell Signaling Technology, Cat#2569S; RRID:AB_2199816), Rabbit recombinant monoclonal anti-Stat3 (1:100, Cell Signaling, Cat# 4904, RRID:AB_331269),

(5) Generation of human pineal gland organoids with melatonin production for disease modelingCell Stem CellJanuary 8, 2026Ferdi Ridvan Kiral, Woo Sub Yang, Onur Iyilikci, Xiaona Lu, Jonghun Kim, Mu Seog Choe, Cynthia Lo, Mei Zhong, Kun-Yong Kim, Yong-Hui Jiang, et al.R&D Systems, AF2669), mouse anti-TTR (1:200, R&D Systems, MAB7505), rabbit anti-TCF7L2 (1:100, Cell Signaling, 2569S), mouse anti-LHX4 (1:500, Millipore Sigma, SAB1409964), rabbit anti-DDC (1:200, Abcam, AB3905), rabbit

(6) TCF7L2 is essential for the differentiation and invasive function of human extravillous trophoblastCellular and Molecular Life SciencesDecember 10, 2025Joudi Salamah, Elisha Cheeran, Brandt Beck, Zaineb Ahmad, Bum-Kyu Leesc-374266), CGB3 (Santa Cruz, sc-271062), and SDC1 (Santa Cruz, sc-390791), TCF7L2 (Cell Signaling, 2569). GAPDH served as the loading control. After three washes with PBST, the membranes were incubated with

(7) A niche-dependent redox rheostat regulates epithelial stem cell fateNature CommunicationsNovember 28, 2025Xi Chen, Krishnan Raghunathan, Bin Bao, Elsy Ngwa, Andrew Kwong, Zhongyang Wu, Stephen Babcock, Clara Baek, George Ye, Anoohya Muppirala, et al.instructions: 1:50 dilution anti-HIF1α (Novus Biologicals, NB100-134), 1:50 dilution anti-TCF4 (CST, 2569 T). 40 μl of Protein G Dynabeads (Invitrogen, 10003D) were used for each ChIP experiment. The purified

(8) Transcription factor 4 maintains endothelial cell identity by inhibiting endothelial to mesenchymal transitionNucleic Acids ResOctober 29, 2025Gaopeng Xian, Rongbin Zheng, Jie Lv, Sen Zhu, Min Chen, Xinlei Gao, Shenli Yuan, Zhen Bouman Chen, Keith Youker, John P Cooke, et al.digitonin. Samples were incubated with a primary antibody against TCF4 (Cell Signaling Technology, #2569) or SNAI1 (Cell Signaling Technology, #3879) overnight at 4°C. After washing, samples were incubated

(9) Impact of intermittent lead exposure on hominid brain evolutionSci AdvOctober 17, 2025Renaud Joannes-Boyau, Janaina Sena de Souza, Manish Arora, Christine Austin, Kira Westaway, Ian Moffat, Wei Wang, Wei Liao, Yingqi Zhang, Justin W. Adams, et al.196868, 1:500; mouse anti-SOX2, Abcam, ab79351, 1:500; rabbit anti-TCF7L2, Cell Signaling Technology, 2569, 1:100; mouse anti-PAX6, BD Biosciences, BDB561462, 1:500; mouse anti-FOXP2, Millipore, MABE415, 1:250)

(10) A double-negative prostate cancer subtype is vulnerable to SWI/SNF-targeting degrader moleculesbioRxivSeptember 11, 2025Phillip Thienger, Irene Paassen, Xiaosai Yao, Philip D. Rubin, Marika Lehner, Nicholas Lillis, Andrej Benjak, Sagar R. Shah, Alden King-Yung Leung, Simone de Brot, et al.Translational Research Unit (TRU) platform, Bern. The TCF7L2 antibody (Cell Signaling Technologies, cat# 2569) was used for staining. The intensity of nuclear immunostaining was evaluated on whole slide tissue

(11) Diaryl pyrimidine guanidine suppresses hepatocellular carcinoma cell stemness by targeting β-catenin signalingFrontiers in OncologySeptember 1, 2025Xuechun Chen, Dongxuan Ni, Jinghui Cheng, Bin Liang, Ruihan Zhang, Weilie Xiao, Rong Liu(#14C10), p-β-catenin-34/37 (#9561), β-catenin (#9562), p-GSK-3β (#9336), CyclinD1 (#2922), and TCF4 (#2569) were purchased from CST (United States), c-Myc (#9E10) was from Santa Cruz (United States). Reverse

(12) KIFC1 Overexpression Promotes Pancreatic Carcinoma Progression via Stabilising BUB1BJ Cell Mol MedAugust 26, 2025Ao Cui, Ying‐Xue Yu, Mei‐Xue Xiong, Ji‐Yang Wang, Ye‐Qing Zou, Ya‐Qiong Zhu, Long‐Jian Ran, Yu Zhang, Rui‐Xiang Liu, Ming‐Yi Dong, et al.(cat. no. HA60053, HUABIO), β‐catenin (cat. no. #8480, Cell Signaling Technology), TCF‐4 (cat. no. #2569, Cell Signaling Technology), c‐Myc (cat. no. #9402, Cell Signaling Technology), cyclin D1 (cat. no.

(13) Statins exhibit anti-tumor potential by modulating Wnt/β-catenin signaling in colorectal cancerOncotargetJuly 21, 2025Sneha Tripathi, Ekta Gupta, Rutika Naik, Satyajeet Khare, Rafeeq Mir, Siddhesh Kamat, Sanjeev Galande(Cell Signaling Tech, Cat No. 3650), SATB2 (Abcam, Cat No. Ab92447), GAPDH (Abcam, Cat No. G041), TCF4 (CST #2569S), AXIN2 (CST #76G6), PCAF (Santa Cruz #13124), β-Actin (Sigma #A2228), E-cadherin (Abcam #ab40772),

(14) PKA-Mediated Phosphorylation of SFRP4 Promotes Wnt/β-Catenin Activation and Cancer Stemness in Gastric CancerInt J Mol SciJune 11, 2025Yoo-Lim Jhe, Suji Lee, Youjin Jung, Jae-Ho Cheong(Dallas, TX, USA); p-β-catenin (Ser33/37/Thr41) (9561S), Lamin A/C (2032S), p-IRAK4 (11927S), and TCF4 (2569S) were from Cell Signaling Technology (Danvers, MA, USA); SFRP4 (LS-C314315) was from LifeSpan Biosciences,

(15) Specification of human brain regions with orthogonal gradients of WNT and SHH in organoids reveals patterning variations across cell linesCell stem cellJune 5, 2025Scuderi S, Kang TY, Jourdon A, Nelson A, Yang L, Wu F, Anderson GM, Mariani J, Tomasini L, Sarangi V, et al.,R&D Systems,Cat#PP-H7147–00; RRID: AB_2155627 Rabbit monoclonal anti-TCF4/TCF7L2 (C48H11),Cell Signaling,Cat#2569; RRID: AB_2199816 Mouse monoclonal anti-HuC/HuD (16A11),ThermoFisher,A21271; RRID: AB_221448 Mouse

(16) Human assembloid model of the ascending neural sensory pathwayNatureJune 1, 2025Kim JI, Imaizumi K, Jurjuț O, Kelley KW, Wang D, Thete MV, Hudacova Z, Amin ND, Levy RJ, Scherrer G, et al.antibodies were used for staining: anti-FOXG1 (rabbit; M227; 1:400 dilution; Takara), anti-TCF7L2 (rabbit; 2569S; 1:200 dilution; Cell Signaling Technology), anti-PHOX2A (rabbit; ab155084; 1:200 dilution; Abcam),

(17) Auricular malformations are driven by copy number variations in a hierarchical enhancer cluster and a dominant enhancer recapitulates human pathogenesisNat CommunMay 17, 2025Xiaopeng Xu, Qi Chen, Qingpei Huang, Timothy C. Cox, Hao Zhu, Jintian Hu, Xi Han, Ziqiu Meng, Bingqing Wang, Zhiying Liao, et al.13-0042k, 0.5 µg), H3K27ac (Active Motif, catalog no. 39133, 0.5 µg), TCF7L2 (Cell Signaling, catalog no. 2569S, 1 µg), and the mixture was incubated overnight on a nutator at 4 °C. On the second day, pAG-MNase and

(18) Tcf4 regulates secretory cell fate decisions in the small intestine and colon tumors: insights from transcriptomic, histological, and microbiome analysesStem Cell Res TherApril 12, 2025Lucie Janeckova, Monika Stastna, Dusan Hrckulak, Linda Berkova, Jan Kubovciak, Jakub Onhajzer, Vitezslav Kriz, Stela Dostalikova, Tereza Mullerova, Katerina Vecerkova, et al.Fisher Scientific); anti-Tacstd2 (rabbit monoclonal, ab214488, Abcam); anti-Tcf4 (rabbit monoclonal, #2569, Cell Signaling Technology); anti-Tcf4 (rabbit monoclonal, MA5-35,295, Thermo Fisher Scientific). Secondary

(19) The Spatial Transcriptional Activity of Hepatic TCF7L2 Regulates Zonated Metabolic Pathways that Contribute to Liver FibrosisNat CommunApril 10, 2025Iriscilla Ayala, Skanda K. Hebbale, Juho Mononen, Madelaine C. Brearley-Sholto, Christopher E. Shannon, Ivan Valdez, Marcel Fourcaudot, Terry M. Bakewell, Anna Zagorska, Giovanna Romero, et al.(Integrated DNA Technologies). Immunoblot for TCF7L2 was performed using a rabbit monoclonal antibody (Cat#: 2569, Cell Signaling Technology) and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Cat#:

(20) Piperlongumine overcomes osimertinib resistance via governing ubiquitination-modulated Sp1 turnoverJCI InsightMarch 24, 2025Ruirui Wang, Qiang Wang, Jinzhuang Liao, Xinfang Yu, Wei Lip-ERK1/2 (REF.4370; IB: 1:1,000), ERK1/2 (REF.9102; IB: 1:2,000), PAX3 (REF.12412; IB: 1:1,000), TCF4 (REF.2569; IB: 1:1,000), Sp1 (REF.5931; IB:1:1,000), Ub (REF.3936; IB: 1:1,000), and Flag-tag (REF.8146; IB: 1:1,000)

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7/21/2023TCF4/TCF7L2 Antibody for Western Blotting From Cell Signaling Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review

TCF4/TCF7L2 (C48H11) Rabbit mAb

TCF4/TCF7L2 (C48H11) Rabbit mAb from Cell Signaling Technology

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TCF4/TCF7L2 (C48H11) Rabbit mAb from Cell Signaling Technology

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