Cell Signaling
TCF4/TCF7L2 (C48H11) Rabbit mAb
2569
C48H11
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The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying the molecular mechanism of oncogenic WNT signal transduction to identify potential targets for therapeutic intervention of colon cancer. This experiment aims to determine good working antibodies to blot some of the core components of the WNT pathway. This antibody picks up two bands and is excellent for Western blotting.
Western blot
RKO whole cell extract
1:2000 dilution; 16 hr at 4 deg C
5% Milk in TBS-T (0.1% Tween-20)
Donkey anti-Rabbit IgG HRP used at 1:10,000 dilution; 1 hr incubation at RT
None
ECL regular
Whole cell extracts from RKO (human colorectal cancer cell line) was resolved on 4-12% Bis-Tris gel followed by protein transfer to nitrocellulose membrane. This antibody detects two bands at ~58 and ~79 kDa. TCF7L2 null line served as a good control in this experiment.
N/A
Specificity.
None.
Excellent antibody for Western blotting application.