Fig 2: Shared mutation gene analysis. (A) KEGG pathway enrichment analysis of 2547 shared mutant genes. (B) PPI network of 14 hub genes identified in shared mutant gene. (C) Kaplan Meier survival analysis of hub genes THBS2, COL14A1 and COL11A1. (D) Fourteen hub gene mutations in three different ovarian cancer cohorts in the cBioPortal database. Each column represents a tumor sample, and each row represents a hub gene

Fig 3: Differential expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. (A) Western blotting was used to detect the expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. In order to improve the clarity and conciseness of the presentation, we cropped the blots. The original, untreated blots images can be found in Additional file 6. (B-D) Statistical analysis of Western blotting results of CCDC170, COL14A1 and THBS2 proteins. (E-G) The expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells was detected by immunofluorescence assay. Original magnification: 40×. (H-J) Statistical analysis of mean fluorescence density of CCDC170, COL14A1 and THBS2 proteins. The gray values of protein bands and the fluorescence intensity of immunofluorescence were analyzed using Image J software (National Institutes of Health, Bethesda, MD, USA).