ELISA plate was coated with serially diluted Mouse IgE-UNLB (SB Cat. No. 0114-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08), and Goat Anti-Mouse IgE-BIOT (SB Cat. No. 1110-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.