Glut1 Antibody - BSA Free NB110-39113 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemGlut1 Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberNB110-39113
ImmunogenThis Glut1 antibody is made against a synthetic peptide made to an N-terminal region of the human GLUT1 protein (between residues 1-100). [Swiss-Prot# P11166]
Concentration1 mg/ml
TargetGlut1
ApplicationsChemotaxis,IF/IHC,Chromatin Immunoprecipitation,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vitro assay,Flow (Intracellular),Simple Western,Chromatin Immunoprecipitation (ChIP),PCR,Immunocytochemistry/ Immunofluorescence
ReactivityHuman,Mouse,Rabbit,Rat
Quantity0.1 ml (also 0.025 ml)
Conjugate/TagUnconjugated
FormatPBS
HostRabbit
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaCancer, Cardiovascular Biology, Cellular Markers, Diabetes Research, Lipid and Metabolism, Membrane Trafficking and Chaperones, Neuroscience, Plasma Membrane Markers, Signal Transduction
IsotypeIgG
NCBI Full Gene Namesolute carrier family 2 member 1
NCBI Gene AliasesDYT17, CSE, GLUT-1, GLUT, GLUT1DS, DYT18, HTLVR, SDCHCN, PED, DYT9, EIG12, GLUT1
Antibody TypePrimary Antibodies
Molecular Weight54.1 kDa
StorageAliquot and store at -20C or -80C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 ml (also 0.025 ml)

Questions and Answers

What applications has the GLUT1 antibody been used for in recent studies?

The GLUT1 antibody has been shown to work in various applications, including Western blotting, immunofluorescence, and immunohistochemistry. For instance, it was utilized in studies investigating glucose transporter expression in different models, such as in the context of ocular hypertension and glioblastoma stem cells, where it played a role in assessing metabolic changes and cellular responses to hypoxia and other stressors.

Can this antibody be used in studies involving cancer metabolism?

Yes, the GLUT1 antibody has been referenced in studies focusing on cancer metabolism. For example, it was used in research examining the metabolic profiling of MYC-amplified medulloblastoma tumors, where increased GLUT1 expression was associated with altered glucose metabolism in tumor cells. Additionally, it has been implicated in studies of cancer cachexia, highlighting its role in glucose uptake in tumor environments.

Is the GLUT1 antibody effective in detecting changes in glucose transporter levels under hypoxic conditions?

Yes, the GLUT1 antibody has been effectively used to detect changes in glucose transporter levels under hypoxic conditions. In studies of ocular hypertension, significant increases in GLUT1 protein levels were observed in the retina, indicating its role in supporting neuronal metabolic demands during hypoxia.

What species does the GLUT1 antibody react with, and how has this been validated?

The GLUT1 antibody is reactive with several species, including humans, mice, rats, and rabbits. Its reactivity has been validated through various studies, including those that utilized the antibody in different experimental models, confirming its effectiveness across these species in applications such as Western blotting and immunohistochemistry.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Melatonin suppresses ILC2-driven airway hyperreactivity via glutathione-dependent metabolic reprogrammingFront ImmunolMay 11, 2026Jafar Cain, Benjamin P. Hurrell, Stephen Shen, Paul Speliakos, Omid Akbariquantify expression of ILC2 cell surface markers: APC KLRG1(2F1; 1:100; Biologend) and PECy7 GLUT1(NB110-39113; 1:100; Novus). Immunophenotyping of murine BALF samples was performed using the following markers:

(2) Protocol to study the genomic profile of histone lactylation with CUT&RUN assay in tumor-associated macrophagesSTAR ProtocApril 11, 2025Alessandra De Leo, Alessio Ugolini, Filippo VegliaCD49 d PE/Dazzle 1:100,BioLegend,Cat#103625 Anti-rabbit Glut1 PE/Cy5.5 1:100,Novus Biologicals,Cat#NB110-39113 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit 1:400,Invitrogen,Cat#L34966A TruStain FcX (anti-mouse CD16/32)

(3) Fibrillar tau alters cerebral endothelial cell metabolism, vascular inflammatory activation, and barrier function in vitro and in vivoAlzheimer's & dementia : the journal of the Alzheimer's AssociationMarch 1, 2025Guzmán-Hernández R, Fossati S.metalloproteinase 2 (MMP2; abcam 86607, 1:1000), and glucose transporter 1 (GLUT1; Novus Biological NB110‐39113, 1:1000). Normalization was performed using anti‐β actin (Millipore; MAB1501, 1:5000) or VDAC (Proteintech

(4) Apelin-13 attenuates optic nerve damage in glaucomatous mice by regulating glucose metabolismJournal of translational medicineFebruary 18, 2025Zhang Y, Zhao J, Zhou J, Jiang Z, Cheng K, Lei C, Yu L.Japan), NeuN (1:200, anti-NeuN antibody, Cell Signaling Technology, 94403 S, USA), GLUT1 (1:100, Novus, NB110-39113, USA), GLUT3 (1:100, Novus, NBP3-12885, USA), and glucose-6-phosphate dehydrogenase (G6PD) (1:100, GeneTex,

(5) Acetate enables metabolic fitness and cognitive performance during sleep disruptionCell metabolismSeptember 3, 2024He Q, Ji L, Wang Y, Zhang Y, Wang H, Wang J, Zhu Q, Xie M, Ou W, Liu J, et al.

(6) Ocular Inflammation and Oxidative Stress as a Result of Chronic Intermittent Hypoxia: A Rat Model of Sleep ApneaAntioxidants (Basel, Switzerland)July 22, 2024Donkor N, Gardner JJ, Bradshaw JL, Cunningham RL, Inman DM.Signaling,C47H1,1:100 LDH-A,Rabbit,Novus,NBP1-48336,1:100 SIRT-1,Rabbit,Novus,MBP2-27205,1:100 GLUT 1,Rabbit,Novus,NB110-39113,1:50 GLUT 3,Mouse,R&D Systems,MAB1415,1:25 Antibodies used for capillary electrophoresis.

(7) Glucose-driven histone lactylation promotes the immunosuppressive activity of monocyte-derived macrophages in glioblastomaImmunityMay 14, 2024De Leo A, Ugolini A, Yu X, Scirocchi F, Scocozza D, Peixoto B, Pace A, D'Angelo L, Liu JKC, Etame AB, et al.

(8) Disruption of CD47-SIRPα signaling restores inflammatory function in tumor-associated myeloid-derived suppressor cellsiScienceApril 19, 2024Zimarino C, Moody W, Davidson SE, Munir H, Shields JD.

(9) TIGAR Deficiency Blunts Angiotensin-II-Induced Cardiac Hypertrophy in MiceInternational Journal of Molecular SciencesFebruary 19, 2024Xiaochen He, Quinesha A. Williams, Aubrey C. Cantrell, Jessie Besanson, Heng Zeng, Jian-Xiong Chen#,Host,Vender,MW (kDa),Dilution GAPDH,#2118,rabbit,"Cell Signaling, Danvers, MA, USA",37,1:5000 Glut-1,NB110-39113,rabbit,"Novus Biologicals, Centennial, CO, USA",55,1:1000 Glut-4,#2213,mouse,"Cell Signaling, Danvers,

(10) Lactate dehydrogenase A inhibition prevents RANKL-induced osteoclastogenesis by reducing enhanced glycolysisJournal of pharmacological sciencesDecember 1, 2023Nishioku T, Anzai R, Hiramatsu S, Terazono A, Nakao M, Moriyama M.

(11) The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cellsBMC cancerNovember 25, 2023Galiger C, Zohora FT, Dorneburg C, Tews D, Debatin KM, Beltinger C.Proteintech, 1:500), mouse anti-HIF-1α (610958, BD Transduction Laboratories, 1:1000), rabbit anti-GLUT1 (NB110-39113, Novus Biologicals, 1:500), rabbit anti-Hexokinase 2 (22029–1-AP, Proteintech, 1:2000), rabbit anti-LDHA

(12) IGFBP7 Fuels the Glycolytic Metabolism in B-Cell Precursor Acute Lymphoblastic Leukemia by Sustaining Activation of the IGF1R-Akt-GLUT1 AxisInternational journal of molecular sciencesJune 2, 2023Artico LL, Ruas JS, Teixeira Júnior JR, Migita NA, Seguchi G, Shi X, Brandalise SR, Castilho RF, Yunes JA.

(13) 16p11.2 haploinsufficiency reduces mitochondrial biogenesis in brain endothelial cells and alters brain metabolism in adult miceCell reportsMay 30, 2023Béland-Millar A, Kirby A, Truong Y, Ouellette J, Yandiev S, Bouyakdan K, Pileggi C, Naz S, Yin M, Carrier M, et al.

(14) Tissue-specific metabolic profile drives iNKT cell function during obesity and liver injuryCell reportsJanuary 31, 2023Aguiar CF, Corrêa-da-Silva F, Gonzatti MB, Angelim MK, Pretti MA, Davanzo GG, Castelucci BG, Monteiro LB, Castro G, Virgilio-da-Silva JV, et al.

(15) IFNγ signaling in cytotoxic T cells restricts anti-tumor responses by inhibiting the maintenance and diversity of intra-tumoral stem-like T cellsNature communicationsJanuary 19, 2023Mazet JM, Mahale JN, Tong O, Watson RA, Lechuga-Vieco AV, Pirgova G, Lau VWC, Attar M, Koneva LA, Sansom SN, et al.

(16) Macrophage acetyl-CoA carboxylase regulates acute inflammation through control of glucose and lipid metabolismScience advancesNovember 25, 2022Yeudall S, Upchurch CM, Seegren PV, Pavelec CM, Greulich J, Lemke MC, Harris TE, Desai BN, Hoehn KL, Leitinger N.rodent OXPHOS antibody cocktail (1:250; Abcam, ab110413), and anti-GLUT1 (1:1000; Novus Biologicals, NB110-39113). Extracellular flux analysis Extracellular flux analysis was performed as previously described (7).

(17) Destabilizing COXIV in Müller Glia Increases Retinal Glycolysis and Alters Scotopic ElectroretinogramCellsNovember 24, 2022Nsiah NY, Inman DM.,Santa Cruz,SC-74430,1:150 MTCO1,Mouse,Invitrogen,459600,1:100 RBPMS,Rabbit,Genetex,118619,1:300 Vimentin,Chicken,Novus Biologicals,NB300-233,1:250 GLUT1,Rabbit,Novus Biologicals,NB110-39113,1:50 CE LDH-A,Rabbit,Novus Biologicals,NBP1-48336,1:50 CE Kir4.1,Rabbit,ProteinTech,12503-1-AP,1:50

(18) Knockout of TIGAR enhances myocardial phosphofructokinase activity and preserves diastolic function in heart failureJournal of cellular physiologyAugust 1, 2022He X, Zeng H, Cantrell AC, Williams QA, Chen JX.immunoblotting. The PVDF membranes were probed with primary antibodies PFK-1 (sc-377346, Santa Cruz, TX), Glut-1 (NB110-39113, Novus Biologicals, CO), Glut-4 (#2213, Cell Signaling, MA), VCAM-1 (#39036, Cell Signaling, MA), GAPDH

(19) NOX4 regulates TGFβ-induced proliferation and self-renewal in glioblastoma stem cellsMolecular oncologyMay 1, 2022García-Gómez P, Golán I, S Dadras M, Mezheyeuski A, Bellomo C, Tzavlaki K, Morén A, Carreras-Puigvert J, Caja L.were used: NOX4, kindly supplied by Isabel Fabregat [33] (1 : 1000, rabbit); GLUT1 (1 : 500, rabbit, nb110‐39113; Novus Biologicals, Abingdon, UK); LIF (1 : 1000, rabbit, GTX101021; GeneTex, Alton Pkwy Irvine, CA,

(20) Ocular Hypertension Results in Hypoxia within Glia and Neurons throughout the Visual ProjectionAntioxidants (Basel, Switzerland)April 29, 2022Jassim AH, Nsiah NY, Inman DM.within the capillary. Protein analysis for GLUT1 (1:50, rabbit, Novus Biologicals, Littleton, CO, USA, NB110-39113), GLUT3 (1:25, mouse, R & D systems, Minneapolis, MN, USA, NAB1415), HIF-1α (1:1000, mouse, Santa Cruz,

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