Fig 1: USP22 mediates PIC stability at ER stress response genes ASchematic of the CHOP locus. Black boxes indicate amplicons for subsequent ChIP‐qPCR analysis.BHCT116 cells were treated for 2 h with 100 nM Thapsigargin in the presence or absence of shRNA targeting USP22. Cells were cross‐linked, harvested, and subjected to ChIP‐qPCR for total RNA Pol II, pSer5 Pol II, and pSer2 Pol II at the loci across CHOP (indicated in (A)). Three independent experiments are represented as mean ± SEM.CChIP‐qPCR for total RNA Pol II, pSer5 Pol II, and pSer2 Pol II at ERP70, GRP78, and a gene desert (Prom = gene promoter and Coding = downstream coding region—see Appendix Table S1 for primer sequences). Three independent experiments are represented as mean ± SEM. with significance measured by Student’s t‐test. *P < 0.05, **P < 0.01, and ***P < 0.005.D–GCells were treated as in (B) and subjected to ChIP‐qPCR for TBP, TFIIB, TFIIF, and TAF7 at the indicated gene promoters. Three independent experiments are represented as mean ± SEM, with significance measured by Student’s t‐test. *P < 0.05 and ***P < 0.005.HAn aliquot of cells from (D‐G) were harvested for whole‐cell lysate and subjected to immunoblotting with the indicated antibodies. Source data are available online for this figure.