CD3 Antibody (SP7) NB600-1441 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemCD3 Antibody (SP7)
CompanyNovus Biologicals, a Bio-Techne Brand
Price $719.00Supplier Page View Company Product Page
Catalog NumberNB600-1441
ImmunogenThis CD3 antibody was developed against a synthetic peptide: KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon chain
IsotypeIgG
ReactivityCanine,Human,Mouse,Porcine
Conjugate/TagUnconjugated
Quantity0.5 ml (also 0.1 ml)
ApplicationsWB, FCM, ICC, IF, IHC, IHC-fr, IHC-p
PurityTissue culture supernatant
Research AreaAdaptive Immunity, Apoptosis, Autoimmune Diseases, Diabetes Research, Immunology, Inflammation, Innate Immunity, Signal Transduction, Stem Cells
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene NameCD3 epsilon subunit of T-cell receptor complex
NCBI Gene AliasesCD3epsilon, IMD18, T3E, TCRE
Molecular Weight23.1 kDa
Antibody TypePrimary Antibodies
TargetCD3
ClonalityMonoclonal
FormatTissue culture supernatant
HostRabbit
CloneSP7

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: The CD3 antigen is present on early thymocytes and mature T cells and is generally regarded as a pan-T cell marker. This antibody will help detect CD3 expression in normal and neoplastic tissues. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

Sizes Available: 0.5 ml (also 0.1 ml)

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Questions and Answers

What species does this CD3 antibody work with in immunohistochemistry applications?

The CD3 antibody (SP7) has been shown to work effectively in immunohistochemistry applications with various species, including humans, mice, and canines, as indicated in multiple studies.

Has this antibody been used in any specific research contexts or disease models?

Yes, the CD3 antibody has been utilized in studies related to neurodegeneration, particularly in the context of tauopathies, where it was used to assess T cell infiltration in mouse models of Alzheimer's disease (). It has also been employed in research on lung cancer, where it was part of a multiplexed quantitative immunofluorescence analysis to evaluate T cell responses in non-small cell lung carcinoma ().

What is the recommended dilution for using this antibody in immunofluorescence?

In immunofluorescence applications, the CD3 antibody (SP7) is typically used at a dilution of 1:10, as demonstrated in various studies ().

Are there any notable findings regarding T cell behavior when using this antibody in research?

Research utilizing the CD3 antibody has revealed significant insights into T cell behavior, such as the identification of a "dormant" T cell phenotype associated with sensitivity to immune checkpoint blockers in lung cancer, highlighting the antibody's role in understanding T cell dynamics in tumor environments ().

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Corneal Immune Cell Alterations and Tau Pathology in a Mouse Model of Alzheimer's DiseaseInvest Ophthalmol Vis SciMay 11, 2026Di Zhang, Duntao Huang, Junju He, Yong Shen, Linbin Dai, Lei Shianti-TUJ1 (Biolegend, #801201), rabbit anti-TUJ1 (Sigma, #T2200), rabbit anti-CD3 (Novus Biologicals, #NB600-1441), rat anti-CD45 (BD Pharmingen, #553076), PE anti-mouse MHC class II (Biolegend, #107607), PE anti-mouse CD11c

(2) Alternatively activated neutrophils limit T cell–driven neuroinflammationJournal of NeuroinflammationMarch 27, 2026Jeffrey R. Atkinson, Calli Bellinger, Andrew D. Jerome, Ashley Munie Gardner, Hayley K. Groover, Shuo Chen, Arnetta McVey Moffatt, Jesse A. Sepeda, Amy Dokiburra, Luke A. Hammond, et al.containing αArg1 (1:100; R&D Systems, Cat. No. IC5868F), αCD3ε (1:100; Novus Biologicals, Cat. No. NB600-1441), and αLy6G (1:200; eBioscience, Cat. No. 16–9668-82). Corresponding secondary antibodies were applied

(3) A sex-biased response of the adult brain to Zika virus infection: from down-regulation of neuronal IRF3 to enhanced pathology in adult female miceCell Communication and SignalingFebruary 11, 2026Zeyni Mansuroglu, Violaine Bortolin, Fanny Lambert, Laurine Conquet, Ignacio Garcia-Verdugo, Gaetano Calcagno, Marie Bourdon, Sylvie Souès, Florence Niedergang, Caroline Manet, et al.(Cell Signaling, 4302 S). Primary antibody for immunohistochemistry (IHC): anti-C3 (SP7) (Biotechne, NB600-1441). Secondary antibodies for Western blot: anti-Rabbit HRP (Jackson Immunoresearch, 711-035-152); anti-Mouse

(4) Targeting immune cells in the aged brain with engineered proteins reveals that engineered cytokine IL-10 enhances neurogenesis and improves cognitionImmunityFebruary 10, 2026Paloma Navarro Navarro Negredo, Justin You, Max Hauptschein, Adam B. Schroer, Daniel J. Richard, Gita C. Abhiraman, Andy P. Tsai, Eric D. Sun, Giulia Notarangelo, Julliana Ramirez-Matias, et al.FITC anti-mouse CD8a Antibody,BioLegend,"Cat# 100803, RRID:AB_312764" Rabbit monoclonal anti-CD3,Novus,"Cat# NB600-1441, RRID:AB_789102" Rat monoclonal anti-CD68,BioLegend,"Cat# 137001, RRID:AB_2044003" Rat monoclonal

(5) Tissue Microarray-Based Digital Spatial Profiling of Benign Breast Lobules and Breast Cancers: Feasibility, Biological Coherence, and Cross-Platform BenchmarksCancers (Basel)November 27, 2025Mark E. Sherman, Jodi C. Carter, Robert A. Vierkant, Melody Stallings-Mann, Laura Pacheco-Spann, Stacey J. Winham, Celine M. Vachon, Chen Wang, Matthew R. Jensen, Melissa A. Troester, et al.1 h: ER: Abcam–ab16660; Ki67: Agilent/Dako–M1740; anti-mouse BCL2: Agilent/Dako–IR61461; CD3: Novus–NB600-1441, titered on human tonsil, anti-rabbit; CD45: Agilent/Dako–IR75161; p16: Abcam Ab54210 1:1600; anti-mouse,

(6) Defining the role of β-cell IRE1α/XBP1 pathway and its gene regulatory network components in non-obese diabetic miceNature CommunicationsNovember 26, 2025Hugo Lee, Khagani Eynullazada, Qiaodan Ou, Junha Shin, Sushmita Roy, Feyza Engin(Santa Cruz, sc-74556; 1:50), anti-Ki67 (Cell Signaling, 9129; 1:100), anti-CD3 (Novus Biologicals, NB600-1441; 1:100), anti-CD4 (Cell Signaling, 25229; 1:20), anti-CD8 (Cell Signaling, 98941; 1:150), anti-B220

(7) Improving immunotherapy responses by dual inhibition of macrophage migration inhibitory factor and PD-1JCI InsightOctober 22, 2025Thuy T. Tran, Gabriela Athziri Sánchez-Zuno, Lais Osmani, Jasmine Caulfield, Caroline Naomi Valdez, Marta Piecychna, Lin Leng, Michelle E. Armstrong, Seamas C. Donnelly, Carlo B. Bifulco, et al.heat-induced epitope retrieval. Primary antibodies against CD3 (clone SP7, Novus Biologicals, 1:100, catalog NB600-1441, RRID:AB_789102), CD8 (clone D4W2Z, Cell Signaling Technology, 1:200, catalog 98941S, RRID:AB_2756376),

(8) Comprehensive proteomic and pathological profiling identifies PRAS40 as a novel biomarker and mediator of primary immune checkpoint blockade resistance in non-small cell lung cancerCancer immunology, immunotherapy : CIIOctober 10, 2025Mao N, He C, Kuang J, Wang C, Yang Z.and T lymphocytes. Primary antibodies used for IHC included anti-CD20 (a B cell marker) and anti-CD3 (NB600-1441, Bio-Techne, China, Shanghai) for T cell identification. TLS were defined as lymphoid aggregates consisting

(9) The CEMIP Hyaluronidase is Elevated in Oligodendrocyte Progenitor Cells and Inhibits Oligodendrocyte MaturationASN neuroJanuary 1, 2025Peters A, Yasuhara K, Su W, Matsumoto S, Pham P, Banine F, Harris E, Back SA, Sherman LS.Manufacturer,Catalog,RRID,Host,Target,Dilution Sigma,G3893,RRID:AB_477010,Mouse,GFAP,1:5000 BioLegend,808402,RRID:AB_2564742,Mouse,MBP,1:800 BD Pharmigen,558774,RRID:AB_397117,Rat,PDGFRα,1:100 Novus,NB600-1441,RRID:AB_789102,Rabbit,CD3,1:100 Wako,019-19741,RRID:AB_839504,Rabbit,Iba1,1:300 Sigma,SAB2105467,RRID:AB_10744081,Rabbit,CEMIP,1:100 R&D Systems

(10) iRhom2 deficiency reduces sepsis-induced mortality associated with the attenuation of lung macrophages in miceHistochemistry and Cell BiologyNovember 1, 2024Jihye Kim, Jee Hyun Kim, Younghoon Kim, Jooyoung Lee, Hyun Jung Lee, Seong-Joon Koh, Jong Pil Im, Joo Sung KimCell Signaling Technology, #97778S, 1:150), CD3 (rabbit monoclonal antibody IgG, Novus Biologicals, # NB600-1441, 1:100), Ki-67 (rabbit polyclonal antibody IgG, Abcam, #ab15580, 1: 400), phospho-NF-κB p65 (Ser 536)

(11) Remyelination protects neurons from DLK-mediated neurodegenerationNature communicationsOctober 23, 2024Duncan GJ, Ingram SD, Emberley K, Hill J, Cordano C, Abdelhak A, McCane M, Jenks JE, Jabassini N, Ananth K, et al.

(12) The evolutionarily ancient FOXA transcription factors shape the murine gut microbiome via control of epithelial glycosylationDevelopmental cellAugust 19, 2024Swisa A, Kieckhaefer J, Daniel SG, El-Mekkoussi H, Kolev HM, Tigue M, Jin C, Assenmacher CA, Dohnalová L, Thaiss CA, et al.Epcam,Abcam,ab71916 Rat anti CD45R,BD Biosciences,550286 Rabbit anti CD3 (SP7),Novus Biologicals,NB600-1441 Rat anti Ki67,Invitrogen,50-245-563 Rabbit anti MUC2 (for immunostaining),Santa Cruz,sc-15334 Rabbit

(13) HCMV IE1/IE1mut Therapeutic Vaccine Induces Tumor Regression via Intratumoral Tertiary Lymphoid Structure Formation and Peripheral Immunity Activation in Glioblastoma MultiformeMolecular neurobiologyAugust 1, 2024Yang X, Jiang S, Liu F, Li Z, Liu W, Zhang X, Nan F, Li J, Yu M, Wang Y, et al.

(14) Inhibition of CCl4-induced liver inflammation and fibrosis by a NEU3 inhibitorPloS oneJanuary 1, 2024Pilling D, Martinez TC, Gomer RH.Signaling Technology, Danvers, MA) to detect tissue resident macrophages, anti-CD3 (rabbit clone SP7, NB600-1441, Novus Biologicals, Centennial, CO, USA) to detect T-cells, anti-CLEC4F (goat Ab, AF2784, Novus Biologicals)

(15) Intratumoral IL15 Improves Efficacy of Near-Infrared PhotoimmunotherapyMolecular cancer therapeuticsOctober 2, 2023Fukushima H, Furusawa A, Kato T, Wakiyama H, Takao S, Okuyama S, Choyke PL, Kobayashi H.

(16) Atezolizumab plus stereotactic ablative radiotherapy for medically inoperable patients with early-stage non-small cell lung cancer: a multi-institutional phase I trialNature communicationsSeptember 2, 2023Monjazeb AM, Daly ME, Luxardi G, Maverakis E, Merleev AA, Marusina AI, Borowsky A, Mirhadi A, Shiao SL, Beckett L, et al.AE1/AE3 (catalog # M3515) from DAKO used with a concentration of 0.12 mg/ml, CD3 clone SP7 (catalog # NB600-1441) from Novus biologicals dilution 1:100 (culture supernatant), GZB clone 4E6 (catalog # ab139354) from

(17) Dynamic Intestinal Stem Cell Plasticity and Lineage Remodeling by a Nutritional Environment Relevant to Human Risk for TumorigenesisMolecular cancer research : MCRAugust 1, 2023Choi J, Zhang X, Li W, Houston M, Peregrina K, Dubin R, Ye K, Augenlicht L.all antibodies. Tissues were incubated overnight at 4°C with primary CD3 antibody (Novus catalog no. NB600-1441, RRID:AB780192, 1:10 dilution). Protein block and secondary antibody for CD3 employed ImmPRESS- Horse

(18) Stress promotes the infiltration of peripheral immune cells to the brainBrain, behavior, and immunityJuly 1, 2023Medina-Rodriguez EM, Han D, Lowell J, Beurel E.

(19) BiTE-Secreting CAR-γδT as a Dual Targeting Strategy for the Treatment of Solid TumorsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)June 1, 2023Huang SW, Pan CM, Lin YC, Chen MC, Chen Y, Jan CI, Wu CC, Lin FY, Wang ST, Lin CY, et al.were purchased from Proteintech and Agilent Technologies, respectively. Antibodies specific for CD3ε (NB600‐1441), PD‐L1 (FAB1561R, FAB1561G), CD4 (FAB3791R), and CD8 (NBP2‐34590AF488) were purchased from Novus Biologicals.

(20) The First Transcriptomic Atlas of the Adult Lacrimal Gland Reveals Epithelial Complexity and Identifies Novel Progenitor Cells in MiceCellsMay 21, 2023Delcroix V, Mauduit O, Lee HS, Ivanova A, Umazume T, Knox SM, de Paiva CS, Dartt DA, Makarenkova HP.(#sc-17764, RRID:AB_628381, Santa Cruz Biotechnology, Inc, Dallas, TX, USA), and CD3 rabbit monoclonal (Cat# NB600-1441, RRID:AB_789102, Novus Biologicals/Bio-Techne, Minneapolis, MN, USA). The samples were washed with TBST

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CD3 Antibody (SP7)

CD3 Antibody (SP7)

CD3 Antibody (SP7)

CD3 Antibody (SP7) from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

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CD3 Antibody (SP7) from Novus Biologicals, a Bio-Techne Brand

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