Pol II (8WG16) Antibody sc-56767 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemPol II (8WG16) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $333.00Supplier Page View Company Product Page
Catalog Numbersc-56767
NCBI Gene AliasesRPO2, Rpb1, RPOL2, POLRA, hsRPB1, 220kDa, Rpo2-1, hRPB220, NEDHIB, RpIILS, POLR2, RPBh1, RPB1
Antibody TypePrimary Antibodies
Quantity200 µg/ml
Clone8WG16
ApplicationsWB, IP, IF
Reactivitymouse, rat, human, Xenopus, C. elegans, yeast, T. aestivum, bovine
FormatPurified
Conjugate/TagUnconjugated
Hostmouse
ClonalityMonoclonal
NCBI Full Gene NameRNA polymerase II subunit A, polymerase (RNA) II (DNA directed) polypeptide A
TargetRNA polymerase II
IsotypeIgG2a

Questions and Answers

Can this antibody be used for studying transcriptional regulation in yeast models?

Yes, the Pol II (8WG16) Antibody has been shown to work in yeast models, specifically in studies investigating RNA polymerase II stalling events during transcription in Saccharomyces. It was utilized in chromatin immunoprecipitation experiments to analyze transcriptional regulation under stress conditions ().

Is this antibody effective for immunostaining in meiotic studies?

The Pol II (8WG16) Antibody has been successfully used for immunostaining in meiotic chromosome spreads, as demonstrated in research focused on meiotic sex chromosome inactivation. It was applied at a dilution of 1:100 in the immunostaining protocol ().

How does this antibody perform in Western blot applications?

The Pol II (8WG16) Antibody has been cited in multiple studies for use in Western blotting, serving as a reliable loading control for various protein analyses, including studies on androgen receptor signaling and transcriptional activity in cancer cells (, ).

What is the reactivity profile of this antibody, and does it cover a wide range of species?

The Pol II (8WG16) Antibody exhibits reactivity with multiple species, including human, mouse, rat, bovine, C. elegans, yeast, and even plant species. This broad reactivity makes it suitable for diverse experimental applications across different biological systems.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) SYCP2 recruits HORMAD2 to chromosome axes for unsynapsed chromatin silencing and synapsis surveillance in meiosisNat CommunJuly 23, 2026Kavya Raveendran, Sarai Valerio-Cabrera, Arkasarathi Gope, Vladyslav Telychko, Geen George, Christin Richter, Tanja Scholte, Matthias Weigel, Anastasiia Bondarieva, Andreas Petzold, et al.Millipore, 1:6000), goat anti-ATR (sc-1887, Santa Cruz, 1:50), mouse anti-RNA polymerase II clone 8WG16 (sc-56767, Santa Cruz Biotechnology, 1:50). Slides were then washed three times with PBS-TT and incubated for

(2) Inhibition of RNA polymerase II-activating CDK9 and CDK12/13, but not of cell cycle relevant CDKs, induces apoptosis by downregulating the short-lived Bcl-2 proteins Mcl1 and Bfl1/A1Cell Death DisMay 27, 2026Karina S. Krings, Judith Hatzfeld, Sandra Weller, Nadine Borkowski, Tanya R. Llewellyn, Laura Schmitt, Bo Scherer, Harri M. Itkonen, Christoph Peter, Björn Stork, et al.#10705-1-AP, 1:2000 anti-PARP (Cell Signaling Technology, #9542S, 1:1000), anti-RNA Pol. II (Santa Cruz, sc-56767, 1:1000), anti-RNA pol. II Ser2 (Abcam, ab5095, 1:1000), anti-RNA pol. II Ser5 (Abcam, #ab5408, 1:500),

(3) Adaptive Regulation of dNTP Homeostasis Confers Osimertinib Resistance in EGFR-Mutant Non–Small Cell Lung CarcinomaCancer ResApril 6, 2026Qian Xie, Yingying Wang, Anthony Fernandez, Yi Lei, Yuanhang Gong, Jessica D. Hess, Lin Yang, Di Xin, Terence M. Williams, Li Zheng, et al.RRID:AB_2878605), and TNNT3 (#19729–1-AP, RRID:AB_2878601) were purchased from Proteintech. Pol II (8WG16; #sc-56767, RRID:AB_785522) and normal mouse IgG (#sc-2025, RRID:AB_737182) were purchased from Santa Cruz Biotechnology.

(4) Fragile nucleosomes are essential for RNA Polymerase II to transcribe in eukaryotesbioRxivFebruary 27, 2026Lingbo Li, Samuel Hunter, Sonia Leach, Yonghua Zhuang, Haolin Liu, Junfeng Gao, Qianqian Zhang, Timothy J. Stasevich, Hiroshi Kimura, Robin Dowell, et al.cat.no.ab252852), 1:100; Native Rpb1 (native Pol II): mouse monoclonal antibody (8WG16, Santa Cruz cat.no.sc-56767), 1:100; Ser2ph-CTD of Pol II: mouse monoclonal antibody (3E10, Abcam cat.no.ab252855), 1:100; H3S10ph:

(5) Enhancer/Enhanceosome/Super-enhancer and the promoter-proximate RNA Polymerase II (Pol II) pausing are coupledbioRxivDecember 11, 2025Haolin Liu, Lingbo Li, Qianqian Zhang, Christopher Ott, Gongyi Zhangfrom Dr. David Bentley Lab as previously reported (Liu, Ramachandran et al. 2020). The Rpb1 antibody (sc-56767) and Actin antibody (sc-8432) were from Santa Cruz Biotechnology. The p300 antibody (#54062) is from

(6) Kinome Reprogramming of G2/M Kinases and Repression of MYCN Contribute to Superior Efficacy of Lorlatinib in ALK-Driven NeuroblastomaMolecular Cancer TherapeuticsSeptember 2, 2025Smita Matkar, Michael P. East, Timothy J. Stuhlmiller, Gabriela M. Witek, Alvin Farrel, Steven Pastor, Denis O. Okumu, Anne Kennedy, Joshua R. Kalna, Esther R. Berko, et al.antibodies [(MYCN from Active Motif (RRID: AB_2793543), Pol II antibody from Santa Cruz Biotechnology (sc-56767, RRID: AB_78522), mouse IgG from Invitrogen (RRID: AB_2532951), and rabbit IgG from Cell Signaling Technology

(7) Kinome Reprogramming of G2/M Kinases and Repression of MYCN Contribute to Superior Efficacy of Lorlatinib in ALK-Driven NeuroblastomaMol Cancer TherFebruary 5, 2025Smita Matkar, Michael P. East, Timothy J. Stuhlmiller, Gabriela M. Witek, Alvin Farrel, Steven Pastor, Denis O. Okumu, Anne Kennedy, Joshua R. Kalna, Esther R. Berko, et al.antibodies [(MYCN from Active Motif (RRID: AB_2793543), Pol II antibody from Santa Cruz Biotechnology (sc-56767, RRID: AB_78522), mouse IgG from Invitrogen (RRID: AB_2532951), and rabbit IgG from Cell Signaling Technology

(8) PhpCNF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA productionNat CommunJanuary 2, 2025Manjit Kumar Srivastav, H. Diego Folco, Patroula Nathanailidou, Anupa T Anil, Drisya Vijayakumari, Shweta Jain, Jothy Dhakshnamoorthy, Maura O'Neill, Thorkell Andresson, David Wheeler, et al.polymerase II immunoprecipitation, 25 μl of anti-Pol II antibody (8WG16, Santa Cruz Biotechnology, sc56767) was used. For Raf2-Myc immunoprecipitation, 25 μl of c-Myc antibody (Santa Cruz Biotechnology, Cat#9E10)

(9) Structural basis of the human transcriptional Mediator regulated by its dissociable kinase moduleMolecular CellOctober 17, 2024Ti-Chun Chao, Shin-Fu Chen, Hee Jong Kim, Hui-Chi Tang, Hsiang-Ching Tseng, An Xu, Leon Palao, Subash Khadka, Tao Li, Mo-Fan Huang, et al.,Cell Signaling,Cat#13641S MED29,Santa Cruz,Cat#sc-393800 Protein A,Sigma-Aldrich,Cat#P1291 RNA Pol II (8WG16),Santa Cruz,Cat#sc-56767 RNA Pol II (Phospho S5),Cell Signaling,Cat#13523 Bacterial and virus strains,Bacterial and virus strains,Bacterial

(10) An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase IIMolecular CellJuly 25, 2024Haiyan Zhao, Jiaqin Li, Yufei Xiang, Sohail Malik, Supriya V. Vartak, Giovana M. B. Veronezi, Natalie Young, McKayla Riney, Jens Kalchschmidt, Andrea Conte, et al.with primary and secondary antibodies using standard procedures. The monoclonal anti-Pol II 8WG16 (sc-56767), anti-RXRα(sc-46659), anti-MED25(sc-393759), and anti-Cdk8(sc-13155) antibodies were from Santa Cruz.

(11) Priming with LSD1 inhibitors promotes the persistence and antitumor effect of adoptively transferred T cellsNature communicationsMay 21, 2024Qiu F, Jiang P, Zhang G, An J, Ruan K, Lyu X, Zhou J, Sheng W.following antibodies were used: anti-LSD1 (Abcam, ab17721), anti-Pol II (Santa Cruz Biotechnology, sc-56767), anti-H3K27ac (Active Motif, 39034), anti-H3K4me1 (Abcam, ab8895), anti-H3K4me2 (EMD Millipore, 07-030),

(12) Novel quinazolin-4(3H)-one based Cyclin K degraders regulate alternative polyadenylation activityBiochemical and biophysical research communicationsOctober 8, 2023Sano O, Ito M, Saito M, Toita A, Tanaka T, Maezaki H, Araki S, Iwata H.

(13) Hnrnpk is essential for embryonic limb bud development as a transcription activator and a collaborator of insulator protein CtcfCell Death & DifferentiationOctober 1, 2023Yuyu Chen, Taifeng Zhou, Zhiheng Liao, Wenjie Gao, Jinna Wu, Shun Zhang, Yongyong Li, Hengyu Liu, Hang Zhou, Caixia Xu, et al.anti-H3K4me3 (CST 9751, 1:1000), anti-H3K27ac (Active Motif 39134, 1:1000), anti-RNA polymerase II (Santa-cruz sc-56767,1:1000), anti-β-ACTIN (Affinity AF7018, 1:2000), goat anti-rabbit IgG H&L (Abcam ab205718, 1:2000),

(14) Sen1 and Rrm3 ensure permissive topological conditions for replication terminationCell reportsJuly 25, 2023Choudhary R, Niska-Blakie J, Adhil M, Liberi G, Achar YJ, Giannattasio M, Foiani M.

(15) Reprogramming of the transcriptome after heat stress mediates heat hormesis in Caenorhabditis elegansNature communicationsJuly 13, 2023Xu F, Li R, von Gromoff ED, Drepper F, Knapp B, Warscheid B, Baumeister R, Qi W.of protein extract was incubated with 2 µg of anti-Pol II 8WG16 (AMA-1) (Santa Cruz Biotechnology, sc-56767), 2 μg anti-POLR2B (RBP-2) (Invitrogen, PA5-30122), 1 μg anti-Histone H3 (Abcam, ab1791) or 1 μg anti-GFP

(16) Mapping Multi-factor-mediated Chromatin Interactions to Assess Dysregulation of Lung Cancer-related GenesGenomics, proteomics & bioinformaticsJune 1, 2023Zhang Y, Zhang J, Zhang W, Wang M, Wang S, Xu Y, Zhao L, Li X, Li G.(Catalog No. 10009D, ThermoFisher Scientific) and 60–100 µg of antibodies against RNAPII (Catalog No. SC-56767, Santa Cruz Biotechnology, Dallas, TX), EZH2 (Catalog No. 5246, Cell Signaling Technology, Danvers,

(17) Activation of P53 pathway contributes to Xenopus hybrid inviabilityProceedings of the National Academy of SciencesMay 23, 2023Zhaoying Shi, Guanghui Liu, Hao Jiang, Songyuan Shi, Xuan Zhang, Yi Deng, Yonglong ChenSonicated chromatin fragments were immunoprecipitated with 3 μg anti-P53 (ab16465, Abcam), anti-Rpb1 (sc-56767, Santa Cruz Biotechnology), anti-H3K27ac (39685, Active Motif), anti-H3K4me1 (39635, Active Motif),

(18) Drosophila DAxud1 Has a Repressive Transcription Activity on Hsp70 and Other Heat Shock GenesInternational journal of molecular sciencesApril 19, 2023Zuñiga-Hernandez J, Meneses C, Bastias M, Allende ML, Glavic A.Polymerase II antibody directed to the unphosphorylated heptapeptide repetition domain in CTD 8WG16 (cat. sc-56767, Santa Cruz Biotechnology, Paso Robles, CA, USA), 6 µg for chromatin from ten pairs of salivary glands),

(19) The Influence of Edaphic Factors on DNA Damage and Repair in Wild Wheat Triticum dicoccoides Körn. (Poaceae, Triticeae)International journal of molecular sciencesApril 6, 2023Raskina O, Shklyar B, Nevo E.1:1000 dilution), mouse monoclonal anti-Pol II antibody (Pol II (8WG16) Alexa Fluor®546 conjugated (#SC-56767 AF546, Santa Cruz Biotechnology, Inc., USA; 1:100 dilution), goat anti-DNA-RNA hybrid [S9.6] FluoroProbes®647

(20) A combinatorial approach to uncover an additional Integrator subunitCell reportsMarch 28, 2023Offley SR, Pfleiderer MM, Zucco A, Fraudeau A, Welsh SA, Razew M, Galej WP, Gardini A.anti-Rabbit IgG (Cell Signaling 2729), anti-GAPDH (Cell Signaling 2118), anti-RNA Polymerase II (Santa Cruz sc-56767), anti-INTS14 (Proteintech 25227-1-AP). INTS15 antibodies serum 1 and serum 2 were raised against recombinant

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Pol II (8WG16) Antibody

Pol II (8WG16) Antibody from Santa Cruz Biotechnology, Inc.

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Questions and Answers

Santa Cruz Biotechnology, Inc.

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Pol II (8WG16) Antibody from Santa Cruz Biotechnology, Inc.

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