RPA 32 kDa subunit (9H8) Antibody sc-56770 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemRPA 32 kDa subunit (9H8) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-56770
Antibody TypePrimary Antibodies
NCBI Gene AliasesRPA32, RPA34, Rf-A2, RP-A p34, REPA2, RP-A p32
Quantity200 µg/ml
Clone9H8
ApplicationsWB, IP, IF, IHC(P), ELISA
Reactivitymouse, rat, human
ClonalityMonoclonal
FormatPurified
Hostmouse
Conjugate/TagUnconjugated
NCBI Full Gene Namereplication protein A2
TargetRPA 32 kDa subunit
IsotypeIgG1

Questions and Answers

What applications has the RPA 32 kDa subunit (9H8) antibody been used for in scientific research?

The RPA 32 kDa subunit (9H8) antibody has been shown to work in various applications including Western Blotting, ELISA, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation.

Can this antibody be used to study the role of RPA2 in DNA damage response?

Yes, the RPA 32 kDa subunit (9H8) antibody has been utilized in studies investigating the DNA damage response, including its role in homologous recombination and the activation of ATR signaling pathways during replication stress, as evidenced by its use in multiple publications.

Is the RPA 32 kDa subunit (9H8) antibody effective in detecting RPA2 in different species?

The antibody is reactive with multiple species, including humans, mice, and rats, making it suitable for comparative studies across these organisms.

How has the RPA 32 kDa subunit (9H8) antibody contributed to understanding cancer therapies?

Research utilizing the RPA 32 kDa subunit (9H8) antibody has highlighted its role in DNA repair mechanisms, which is crucial for understanding the sensitivity of cancer cells to therapies such as PARP inhibitors, as seen in studies focusing on homologous recombination defects in cancer.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) LIPT1 loss confers replication stress and PARP inhibitor sensitivity through PrimPol-mediated ssDNA gapsSci AdvMay 1, 2026Zengfu Shang, Jui-Chung Chiang, Ching-Cheng Hsu, Ciara Newman, Anthony J. Davis, Yuanyuan Zhangwith antibodies for Pol ε catalytic subunit (NBP2-55332, Novus Biologicals, Centennial, CO) and RPA32 (sc-56770, Santa Cruz Biotechnology, Dallas, TX) (1:500 diluted in Duolink in situ antibody diluent), then incubated

(2) PRMT5 is Frequently Upregulated and a Potential Therapeutic Target in MTAP-deficient Malignant Peripheral Nerve Sheath TumorsbioRxivMarch 13, 2026Dingxun Wang, Melissa L. Fishel, Azadeh Samiei, Silpa Gampala, Changdeng Hu, Shaoxiong Chen, GuangJun Zhang(Cell Signaling Technology, cat# 6334, RRID:AB_11178526, 1:1000), anti-RPA32 (Santa Cruz Biotechnology, cat# sc-56770, RRID:AB_785534, 1:200), anti-Rad51 (Cell Signaling Technology, cat# 8875, RRID:AB_2721109, 1:1000),

(3) Combination of PARP and KRASG12D inhibitors enhances therapeutic efficacy by exploiting vulnerabilities in PDACNature CommunicationsFebruary 24, 2026Xin Xu, Xin Chen, Rongli Xu, Zhenyu Huo, Changying Li, Somaira Nowsheen, Khaled Aziz, Fan Yao, Zhenkun Lou, Min Deng(Millipore, 05-1572), FK2 (Millipore, 04-263), BRCA1 (Santa Cruz Biotechnology, sc-6954), RPA32 (Santa Cruz, sc-56770) and RAD51 (GeneTex, GTX100469). Samples were then washed three times and incubated with the corresponding

(4) Poly ADP Ribose Polymerase Inhibitors Potentiate Proton Therapy End-of-Range Effects by Accelerating Replication Forks and Promoting Transcription ConflictInternational Journal of Radiation Oncology*Biology*PhysicsFebruary 1, 2026Yiqun Han, Qin Zhou, Anna Dibbs, Bin Chen, Taylor M. Weiskittel, Nicholas B. Remmes, Zheming Wu, Daniel K. Ebner, Jake A. Kloeber, Cameron M. Callaghan, et al.RRID AB_309864) antibody for IF was purchased from Millipore. Anti-RPA32 kDa subunit antibody (9H8) (sc-56770, RRID AB_785534) for IF was purchased from Santa Cruz Biotechnology. Anti-RAD51 antibody (D4B10) (8875)

(5) Elimusertib, a Novel ATR Inhibitor, Induces Anti-tumor Effects through Replication Catastrophe in Breast CancersCancer Research and TreatmentJanuary 15, 2026Mudong Kim, Ahrum Min, Sohyeon Kim, Seongyeong Kim, Yu-jin Kim, Sujin Ham, Miso Lee, Eunice Yoojin Lee, Jinyong Kim, Dae-Won Lee, et al.Biosciences). The following is a list of primary antibodies used in this study: anti-RPA32 (mouse, sc-56770, Santa Cruz Biotechnology), anti-caspase 7 (rabbit, #9492, Cell Signaling Technology), anti-pH2AX (mouse,

(6) PAK1 inhibition synergistically enhances the anti-tumor efficacy of PARP inhibitors in ovarian cancersGenes & DiseasesOctober 21, 2025Changying Li, Xinyan Li, Ming Gao, Min Deng, Ye-Xiong Li, Zhenkun LouAntibodies were sourced as follows: anti-FLAG (F1804, 1:2000) was obtained from Sigma; anti-RPA32 (sc-56770, 1:2000) was obtained from Santa Cruz; anti-pS345 Chk1 (2348, 1:1000), anti-phospho-PAK1 (Thr423) (2601,

(7) Loss of Nuclear Profilin 1 Triggers Oncogenic Reprogramming of Mammary Epithelial Cells Through Dysregulated DNA Replication in Breast CancerJournal of Breast CancerOctober 17, 2025Zheng Zhao, Yingbin Huang, Junhao Mai, Fei Cao, Qi Fang, Di Wu, Ziqian Li, Xuekui LiuThe primary antibody used for immunofluorescence was mouse anti-replication protein A 32-kDa (RPA32, #sc-56770; Santa Cruz). The secondary antibodies used for western blotting were horseradish peroxidase-conjugated

(8) Isoform-specific function of NSD3 in DNA replication stress confers resistance to PARP inhibitors in prostate cancerMolecular CellJuly 17, 2025Shouhai Zhu, Huanyao Gao, Dan Jiang, Guijie Guo, Jing Hou, Yiqun Han, Chao Zhang, Xiaoping Hu, Shreya Indulkar, Jake A. Kloeber, et al.anti-BLM,Abcam,Cat#ab2179; RRID: AB_2290411 Mouse monoclonal anti-RPA2,Santa Cruz Biotechnology,Cat#sc-56770; RRID: AB_785534 Rabbit polyclonal anti-SRSF10,Proteintech,Cat#10131–1-AP; RRID: AB_2877726 Mouse

(9) CRAMP1-dependent histone H1 biogenesis is essential for topoisomerase II inhibitor toleranceMolecular cellJuly 3, 2025Ingham A, de Vega IA, Morlot L, Gittens W, Hendriks IA, Kakulidis ES, Freire R, Davey NE, Duxin JP, Lund Nielsen M, et al.(Ser33),Novus,"(Novus Cat# NB100-544, RRID:AB_10002227)" RPA2,Santa Cruz Biotechnology,"(Santa Cruz Biotechnology Cat# sc-56770, RRID:AB_785534)" ß-Actin,Sigma-Aldrich,"(Sigma-Aldrich Cat# MAB1501, RRID:AB_2223041)" SLBP,Abcam,(Abcam

(10) Unveiling transcriptional mechanisms of B7-H3 in breast cancer stem cells through proteomic approachesiScienceApril 18, 2025Yu Ri Seo, Han Byeol Kim, Hyeryeon Jung, Eunhee G. Kim, Sumin Huh, Eugene C. Yi, Kristine M. Kim,Santa Cruz Biotechnology,Cat# sc-48425; RRID:AB_628225 Mouse monoclonal anti-RPA3,Santa Cruz Biotechnology,Cat# sc-56770; RRID:AB_785534 Goat polyclonal anti-B7H3,R&D Systems,Cat# AF1027; RRID:AB_354546 Rabbit monoclonal

(11) Condensin II interacts with BLM helicase in S phase to maintain genome stabilityCommun BiolMarch 25, 2025Brian Rodemoyer, Ganesha Kariyawasam, Veena Subramanian, Kristina Schmidtno. 9701S, WB, 1:500), RAD51 (Santa Cruz, cat. no. sc-398587, WB, 1:200), RPA2 (Santa Cruz, cat. no. sc-56770, WB, 1:200), SMC2 (Bethyl, cat. no. A300-058A, IF and Immunoprecipitation (IP), 1:1000 and 5 μg/mL;

(12) γ-tubulin mediates DNA double-strand break repairJournal of Cell ScienceMarch 15, 2025Abhishikt David Solomon, Odjo G. Gouttia, Ling Wang, Songli Zhu, Feifei Wang, Yanqui Li, Mohammadjavad Paydar, Tadayoshi Bessho, Benjamin H. Kwok, Aimin Penganti-histone H3 (1G1, sc-517576, 1:1000), anti-DNA polymerase λ (E-11, sc-373844, 1:1000), anti-RPA32 (9H8, sc-56770, 1:1000) and anti-RPA70 (H-7, sc-48425, 1:1000) from Santa Cruz Biotechnology (Dallas, TX, USA). The

(13) Mono-ubiquitination of TopBP1 by PHRF1 enhances ATR activation and genomic stabilityNucleic acids researchFebruary 27, 2025Zhao F, Cai C, Gao H, Moon J, Christyani G, Qin S, Hao Y, Liu T, Lou Z, Kim W.1:2000), anti-phospho RPA32 (S33) (Bethyl Laboratories: A300-246A-M, 1:2000), anti-RPA32 (Santa Cruz: sc-56770, 1:2000), anti-Ub (Santa Cruz: sc:8017, 1:2000), anti-α-tubulin (Sigma: T6557, 1:5000), anti-GAPDH (Proteintech:

(14) A CRISPR-Cas9 screen reveals genetic determinants of the cellular response to decitabineEMBO ReportsFebruary 10, 2025Pinqi Zhang, Zhuqiang Zhang, Yiyi Wang, Wenlong Du, Xingrui Song, Weiyi Lai, Hailin Wang, Bing Zhu, Jun Xiong,Santa Cruz,sc-20701 DNMT1,ABclonal,A16729 UHRF1,Santa Cruz,sc-373750 KDM1A,ABcam,ab17721 γH2A.X,ABcam,ab22551 γH2A.X,ABcam,ab2893 RPA32,Santa Cruz,sc-56770 ZMYM2,Thermo Scientific,PA5-28265 ZMYM3,Thermo Scientific,PA5-51862 RCOR1,Sigma,07-455 RCOR2,ABcam,ab37113 HDAC1,Proteintech,6085-1-Ig ACTB,ABclonal,AC026 GFP,ABclonal,AE011 GFP,ABcam,ab13970 GFP,MBL,598 H3,ABclonal,A2348 FLAG,Sigma,B3111 PARylation,Santa Cruz

(15) Heterozygous RPA2 variant as a novel genetic cause of telomere biology disordersGenes & developmentSeptember 19, 2024Kochman R, Ba I, Yates M, Pirabakaran V, Gourmelon F, Churikov D, Laffaille M, Kermasson L, Hamelin C, Marois I, et al.PVDF membranes (Millipore). Detection was performed using mouse anti-RPA2 (Santa Cruz Biotechnology sc-56770) and mouse anti-HA (Santa Cruz Biotechnology sc-7392). Homologous recombination-mediated repair assay RPE1

(16) AXL inhibition prevents RPA2/CHK1-mediated homologous recombination to increase PARP inhibitor sensitivity in hepatocellular carcinomaHeliyonSeptember 15, 2024Kai-Min Li, Li-gong Deng, Li-Jun Xue, Chang Tan, Shu-Kun YaoFor Immunoblotting, the following primary antibodies were used: Anti-AXL (sc-166269, 1:2000) and Anti-RPA2 (sc-56770, 1:2000) were procured from Santa Cruz; Anti-RPA1 (A300–241A, 1:5000) was obtained from Bethyl Laboratories.

(17) The Compromised Fanconi Anemia Pathway in Prelamin A‐Expressing Cells Contributes to Replication Stress‐Induced Genomic InstabilityAdvanced ScienceAugust 1, 2024Pengqing Nie, Cheng Zhang, Fengyi Wu, Shi Chen, Lianrong Wang67304‐1‐Ig), anti‐RAD51 (Proteintech, 14961‐1‐AP), anti‐53BP1 (ABclonal, A5757), anti‐RPA2 (Santa Cruz, sc‐56770), rabbit control IgG (ABclonal, AC005) and mouse control IgG (ABclonal, AC011) were used. LPV (MCE,

(18) USP25 Elevates SHLD2-Mediated DNA Double-Strand Break Repair and Regulates Chemoresponse in CancerAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2024Li Y, Li L, Wang X, Zhao F, Yang Y, Zhou Y, Zhang J, Wang L, Jiang Z, Zhang Y, et al.(Millipore: 05–636, IF: 1: 1000); anti‐phospho‐RPA32(BETHYL: A300‐245A, WB: 1:500); anti‐RPA32 (Santa Cruz: sc‐56770, IF: 1:500); anti‐RAD51 (GENETEX: GTX100469, IF: 1:500); anti‐53BP1 (Novus Biologicals: NB100‐304, WB:

(19) Syk-dependent homologous recombination activation promotes cancer resistance to DNA targeted therapyDrug Resistance UpdatesMay 1, 2024Qin Zhou, Xinyi Tu, Xiaonan Hou, Jia Yu, Fei Zhao, Jinzhou Huang, Jake Kloeber, Anna Olson, Ming Gao, Kuntian Luo, et al.1:2000), and anti-CtIP (Thr327) (p1012–327, 1:2000) were purchased from PhosphoSolutions. anti-RPA32 (sc-56770) and anti-CtIP (sc-271339 for WB) were purchased from Santa Cruz; anti-FLAG (F1804) was purchased from

(20) Harmonin homology domain-mediated interaction of RTEL1 helicase with RPA and DNA provides insights into its recruitment to DNA repair sitesNucleic Acids ResearchFebruary 9, 2024Niranjan Kumar, Arushi Taneja, Meenakshi Ghosh, Ulli Rothweiler, Nagalingam Ravi Sundaresan, Mahavir SinghNo. SAB1406399); Anti-RPA 32 kDa subunit (9H8), produced in Mouse (Santa Cruz Biotechnology, Cat. No. sc-56770); Anti-HA tag (C29F4), produced in Rabbit (Cell Signalling Technology, Cat. No. 3724); Anti-gamma H2A.X (phospho S139) antibody [3F2

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RPA 32 kDa subunit (9H8) Antibody

RPA 32 kDa subunit (9H8) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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RPA 32 kDa subunit (9H8) Antibody from Santa Cruz Biotechnology, Inc.

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