Indirect immunostaining of a PFA fixed brain section from a 5XFAD mouse (dilution 1 : 500; green). Immunoreactivity in amyloid plaques was revealed using fluorochromated secondary antibodies. Nuclei have been visualized by DAPI staining.

Immunostaining of paraffin embedded brain section from an Alzheimer's patient (dilution 1 : 100). Immunoreactivity in amyloid plaques was revealed using diaminobenzidine as chromagen. Nuclei were counterstained with haematoxylin (blue).

Courtesy: O. Wirths, Dept. of Psychiatry, Div. of Molecular Psychiatry; University of Goettingen.

ELISA quantification of different Abeta 40 amounts. Identical amounts of Abeta 38 and 42 have been included as specificity controls.
Fig. A: rabbit anti-Abeta 40 (cat. no. 218 203) was used as capture and mouse anti-Abeta (clone NT78, cat. no. 218 111) as detector antibody.
Fig. B: mouse anti-Abeta (clone NT78, cat. no. 218 111) was used as capture and rabbit anti-Abeta 40 (cat. no. 218 203) as detector antibody.