Fig 1: Tas affects DACH1 expression level by regulating PPM1E expression. PPM1E mRNA (A) and proteins (B) were enhanced by Tas. After transfection of pCI−neo or pCI−Tas for 48 h, cells were collected for RT−qPCR or Western blotting to detect DACH1 expression. (C) Tas enhances DACH1 protein expression after interfering with PPM1E via RNAi. PPM1E siRNA (50 nM) was transfected into HeLa cells with pCI−Tas, with siRNA−NC as the siRNA transfection negative control, and untreated Hela cells as the control for the normal experimental group. All data are presented as mean ± SD (n = 3). *** p < 0.001.