Biotin Anti-RFP antibody ab34771 from Abcam

Product Specs
ItemBiotin Anti-RFP antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab34771
HostRabbit
IsotypeIgG
ClonalityPolyclonal
Quantity100 µg
Conjugate/TagBiotin
ApplicationsWB
FormatThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal
Concentration1 mg/ml
Antibody TypePrimary Antibody
TargetRed fluorescent protein drFP583
Research AreaNeuroscience

Questions and Answers

What applications has the Biotin Anti-RFP antibody been used for in scientific research?

The Biotin Anti-RFP antibody has been shown to work in applications such as Western Blot and Immunohistochemistry. It has been utilized to detect Red Fluorescent Protein in various studies, including those examining neuronal activity and gene expression in mouse models (, ).

Can this antibody effectively detect different RFP variants in various experimental contexts?

Yes, the Biotin Anti-RFP antibody has been reported to robustly detect monomeric Cherry and specifically detect tdTomato in ROSA-RFP mice, indicating its versatility in recognizing different RFP variants in various experimental setups ().

Is there any evidence of the antibody's specificity in detecting RFP in complex biological samples?

The antibody has demonstrated specificity, as it was prepared from monospecific antiserum and confirmed through ELISA, showing no reaction against human, mouse, or rat serum proteins at less than 0.1% of target signal. This specificity is crucial for accurate detection in complex biological samples ().

How has the Biotin Anti-RFP antibody been utilized in studies related to neuronal function?

The antibody has been employed in studies investigating neuronal circuits and functions, such as in the examination of GABAergic interneurons in the hippocampus, where it was used to label RFP in various neuronal populations, aiding in the understanding of synaptic interactions and information processing in the brain ().

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(1) Telomerase Knockout in Myeloid Cells Predisposes Mice to Foam Cell Formation, Dyslipidemia, Lung Fibrosis, and Cardiac DysfunctionAging CellApril 16, 2026Zhanguo Gao, Yongmei Yu, David Wiggins, Eva M. Sevick-Muraca, Mikhail G. Kolonin(Cell Signaling, 3470, 1:100), TNNT2 (Santa Cruz, sc‐20,025, 1:100), GFP (GeneTex, GTX22242, 1:200), RFP (Abcam, ab34771, 1:100) and CD31 (Santa‐Cruz, sc‐1506, 1:75) were applied (4°C, 12 h) followed by Donkey Alexa488‐conjugated

(2) Starch Synthase 3 isoforms are essential for normal starch granule initiation in wheat endospermNew PhytologistFebruary 10, 2026Jinjin Ding, Brendan Fahy, Ryo Matsushima, Qiantao Jiang, David Seungimmunoblot detection of fusion proteins, we used anti‐HA (Abcam plc; ab9110–1:5000) and anti‐RFP (Abcam plc; ab34771–1:2000) primary antibodies, and horseradish peroxidase–coupled secondary antibody anti‐rabbit HRP (Sigma;

(3) Brain-Wide Patterns of CRF Receptor 1 Expression in the Transgenic CRFR1-Cre-2a-tdTom RatJ Comp NeurolOctober 30, 2025Douglas S. Engelke, Lierni Ugartemendia, Jonathan Tao, Shivakumar Rajamanikam, Fabricio H. Do Monte, Nicholas J. JusticeX‐100 in PBS containing biotinylated rabbit anti‐red fluorescent protein antibody (1:1000, Abcam, Cat# ab34771, RRID: AB_777699) at 4°C for 48 h. Sections were washed in PBS and incubated in biotinylated goat anti‐rabbit

(4) Sensory Deficits in Mice with Lateral Spinal Cord Hemisection Mimic the Brown-Séquard SyndromeJ NeurosciOctober 29, 2025Melissa Henwood, Junkui Shang, Qiang Li, John Moth, John Henwood, Yang Yi, Dustin Green, Ajay Pal, Joseph Sandoval, Wei Li, et al.[Invitrogen (13-0300), 1:600]; chicken anti-GFP [Abcam (ab13970), 1:400]; and rabbit anti-RFP [Abcam (ab34771), 1:400]. The secondary antibodies used were 647 donkey anti-guinea pig (Jackson ImmunoResearch Laboratories

(5) Pharmacological Microglial Inhibition Remodels the Scar Microenvironment to Support Reticulospinal Circuit Reconstruction After Spinal Cord InjuryAdvanced ScienceOctober 17, 2025Run Li, Hongyuan Xing, Yifan Shen, Meng Chen, Bowen Lyu, Xiaofeng Yang, Li Sun, Chao Jiang, Jianyu Lv, Xin Ding, et al.(Santa Cruz(sc‐32725), 1:100, USA);mouse anti‐IBA1 (ABclonal(A27316), 1:1000, China); rabbit anti‐RFP (Abcam(ab34771), 1:1000, UK); rabbit anti‐P2Y12 (Abcam (Ab300140), 1:1000, UK); rabbit anti‐CD68 (ABclonal (A6554)),

(6) Sensory Deficits in Mice with Lateral Spinal Cord Hemisection Mimic the Brown–Séquard SyndromeJ NeurosciSeptember 16, 2025Melissa Henwood, Junkui Shang, Qiang Li, John Moth, John Henwood, Yang Yi, Dustin Green, Ajay Pal, Joseph Sandoval, Wei Li, et al.[Invitrogen (13-0300), 1:600]; chicken anti-GFP [Abcam (ab13970), 1:400]; and rabbit anti-RFP [Abcam (ab34771), 1:400]. The secondary antibodies used were 647 donkey anti-guinea pig (Jackson ImmunoResearch Laboratories

(7) Anatomical and behavioral characterization of three hemiplegic animal modelsBMC NeurosciJuly 21, 2025Mei Liu, Lingling Xu, Gefei Cheng, Yang Yang, Likun Yang, Yuhai Wangprior to the staining procedure. The primary antibodies, namely rabbit anti-RFP (procured from Abcam [ab34771], 1:1,000) and rabbit anti-GFAP (from DAKO [Z0334], 1:800)] were applied to the samples. These samples

(8) Neuroimmune signaling mediates astrocytic nucleocytoplasmic disruptions and stress granule formation associated with TDP-43 pathology☆Neurobiology of DiseaseJuly 1, 2025Constance Zhou, Evelyn J. Hardin, Till S. Zimmer, Stephanie Jackvony, Daniel Barnett, Noopur Khobrekar, Elisa Giacomelli, Lorenz Studer, Adam L. Orr, Anna G. Orr#AB_10917109), chicken anti-GFAP (1:500; Abcam #ab4674; RRID #AB_304558), rabbit anti-RFP (1:500; Abcam #ab34771; RRID #AB_777699), rabbit anti-dsRed and mCherry (1:500; Takara Bio #632496; RRID #AB_10013483) and

(9) Caloric Restriction and Telomere Preservation in TERT Knockout Adipocyte Progenitors Does Not Rescue Mice From Metabolic Dysfunction due to a TERT Function in Adipocyte MitochondriaAging cellMarch 1, 2025Gao Z, Yu Y, Eckel-Mahan K, Kolonin MG.Cell Signaling, #11967, (1:150), PDGFRβ: Abcam, ab32570 (1:100), GFP: Gene Tex, GTX26673 (1:300), and RFP: Abcam, ab34771 (1:100) primary antibodies (4°C, 12 h) were used followed by Donkey Alexa488‐conjugated (Invitrogen

(10) Spinal projecting neurons in rostral ventromedial medulla co-regulate motor and sympathetic toneCellJune 20, 2024Zicong Zhang, Junfeng Su, Jing Tang, Leeyup Chung, Jessica C. Page, Carla C. Winter, Yuchu Liu, Evgenii Kegeles, Sara Conti, Yu Zhang, et al.at dilution of 1:500 if not otherwise specified: chicken anti-GFP (GFP-1020, Aves), rabbit anti-RFP (ab34771, Abcam), goat anti-cholinergic acetyltransferase (ChAT) (1:200; AB144P, Millipore), rabbit anti-tyrosine

(11) Selective plasticity of layer 2/3 inputs onto distal forelimb controlling layer 5 corticospinal neurons with skilled grasp motor trainingCell ReportsApril 23, 2024Yoshio Takashima, Jeremy S. Biane, Mark H. TuszynskiSections were incubated overnight at room temperature in appropriate primary antibodies: RFP (Abcam, ab34771); GFP (Aves, GFP-1020); NeuN (Millipore, abn90); Calretinin (Encor bio, CPCA-Calretinin); Calbindin

(12) R-Spondin1 protects gastric stem cells and mitigates gastric GVHD in allogeneic hematopoietic stem cell transplantationBlood advancesFebruary 13, 2024Hayase E, Ara T, Saito Y, Takahashi S, Yoshioka K, Ohigashi H, Ogasawara R, Yokoyama E, Yamakawa T, Ebata K, et al.(Abs), including chicken anti-EGFP (ab13970; Abcam, Tokyo, Japan), rabbit anti–red fluorescent protein (ab34771; Abcam), rabbit anti–Ki-67 (MA5-14520; Thermo Fisher Scientific), and rabbit anti-cleaved caspase-3

(13) O-glycosylation of the transcription factor SPATULA promotes style development in ArabidopsisNature PlantsJanuary 26, 2024Yuxiang Jiang, Seamus Curran-French, Samuel W. H. Koh, Iqra Jamil, Benguo Gu, Luca Argirò, Sergio G. Lopez, Carlo Martins, Gerhard Saalbach, Laila MoubayidinThe following antibodies were used: anti-GFP (GF28R, 1:1,000 dilution, Thermo Scientific), anti-RFP (ab34771, 1:2,500 dilution, Abcam), anti-FLAG (F3165, 1:3,000 dilution, Sigma), anti-HA (3F10, 1:3,000 dilution,

(14) A transcriptomic taxonomy of mouse brain-wide spinal projecting neuronsNatureDecember 1, 2023Winter CC, Jacobi A, Su J, Chung L, van Velthoven CTJ, Yao Z, Lee C, Zhang Z, Yu S, Gao K, et al.µm coronal sections, four series). IHC was performed as described above, with rabbit-anti-RFP (Abcam ab34771; 1:1,000) and goat-anti-ChAT (Millipore AP144P; 1:100) primary antibodies, and corresponding secondary

(15) Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1The Plant CellOctober 30, 2023Nitin Uttam Kamble, Farrukh Makhamadjonov, Brendan Fahy, Carlo Martins, Gerhard Saalbach, David SeungSDS-PAGE and immunoblotting, using anti-YFP (Torrey Pines; TP401—1:5,000) and anti-RFP (Abcam plc; ab34771—1:2,000) primary antibodies. Proteins were detected using chemiluminescence from horseradish peroxidase–coupled

(16) Increasing amyloplast size in wheat endosperm through mutation of PARC6 affects starch granule morphologyNew PhytologistOctober 1, 2023Lara Esch, Qi Yang Ngai, J. Elaine Barclay, Rose McNelly, Sadiye Hayta, Mark A. Smedley, Alison M. Smith, David Seungconcentrations: 1 : 5000 anti‐GFP (TP401; Torrey Pines, Biolabs, Secaucus, NJ, USA), 1 : 2000 anti‐RFP (ab34771; Abcam, Cambridge, UK) and 1 : 5000 anti‐HA (ab9110; Abcam). Bands were detected using the anti‐rabbit

(17) Targeting and Sensitization of Breast Cancer Cells to Killing with a Novel Interleukin-13 Receptor α2-Specific Hybrid Cytolytic PeptideCancersMay 16, 2023Jannoo R, Walker W, Kanamarlapudi V.anti-mouse secondary antibody. Where indicated, mCherry was detected with rabbit anti-RFP antibody (Abcam [#ab34771]). Membranes were developed using ECL Select Substrate (GE Healthcare (#RPN2235), Hatfield, UK) and

(18) Quantitative imaging of vesicle-protein interactions reveals close cooperation among proteinsJournal of extracellular vesiclesMay 1, 2023Cha M, Jeong SH, Jung J, Baeg Y, Park SS, Bae S, Lim CS, Park JH, Lee JO, Gho YS, et al.imaging, solutions of NeutrAvidin, biotin‐conjugated antibodies (anti‐GFP: Abcam, ab6658; anti‐RFP: Abcam, ab34771), and the cell lysates containing fluorescent proteins were sequentially injected into a sample chamber,

(19) A PDLP-NHL3 complex integrates plasmodesmal immune signaling cascadesProceedings of the National Academy of SciencesApril 25, 2023Estee E. Tee, Matthew G. Johnston, Diana Papp, Christine FaulknerImmunoblotting was carried out with anti-GFP (Roche, 11814460001; 1:1,000) or anti-RFP-biotin (Abcam, ab34771, 1:2,000) primary antibodies, and anti-mouse IgG (Sigma, A0168; 1:10,000) or anti-rabbit-HRP (Sigma,

(20) Gliotransmission and adenosine signaling promote axon regenerationDevelopmental cellApril 24, 2023Wang F, Ruppell KT, Zhou S, Qu Y, Gong J, Shang Y, Wu J, Liu X, Diao W, Li Y, et al.antibody (Thermo Fisher, #A5–10029, RRID:AB_2556609) as well as rabbit anti-RFP primary antibody (Abcam, #ab34771, RRID: AB_777699) and donkey anti-rabbit Alexa 555-conjugated secondary antibody (Thermo Fisher, A-31572,

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Biotin Anti-RFP antibody from Abcam

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Biotin Anti-RFP antibody from Abcam

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