Fig 1: Identification of meprin-β in the CSF. A Western blot of CSF obtained from the cisterna magna of control wild-type (Ctrl) and KO for Mep1b (Mep1b − / −) mouse, and resolved with meprin-β N-terminal AF2895 and C-terminal NBP2 antibodies. B Representative western blot of human lumbar CSF (control cases) revealed with the N-terminal and C-terminal antibodies previously described. C Immunoprecipitation (IP) of human frontal cortex homogenate against meprin-β using the alternative N-terminal antibody ab42743, and resolved with N-terminal AF2895. Input: brain homogenate; B: bound fraction from the IP; Bc: unspecific bound from control IP. Arrowheads indicate the bands attributed to the zymogen (black) and the mature species generated after cleavage of the pro-domain; (*) unspecific bands. The uncropped blots of the analyzed samples are available as Supplemental Fig. 2
Fig 2: Identification of meprin-β in the brain. A Western blot of brain cortex homogenates from control wild-type (Ctrl) and KO for Mep1b (Mep1b − / −) mouse resolved with meprin-β N-terminal AF2895 and C-terminal NBP2 antibodies. B Representative western blot of human frontal cortex homogenates revealed with the N-terminal and C-terminal antibodies previously described. C Immunoprecipitation (IP) of human frontal cortex homogenate against meprin-β using the alternative N-terminal antibody ab42743, and resolved with N-terminal AF2895. Input: brain homogenate; B: bound fraction from the IP; Bc: unspecific bound from control IP. Arrowheads indicate the bands attributed to the zymogen (black) and the mature species generated after cleavage of the pro-domain; (*) unspecific bands. The uncropped blots of the analyzed samples are available as Supplemental Fig. 1
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