Immunofluorescent analysis of CUTL1 showing staining in the Golgi apparatus of SK-N-SH cells. SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CUTL1 polyclonal antibody (Product # PA5-30003) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.

Western blot was performed using Anti-CUX1 Polyclonal Antibody (Product # PA5-30003) and 166 kDa, 157 kDa, 77 kDa and 72 kDa bands corresponding to CUX1 was observed across all the cell lines tested along with uncharacterized bands at ~22 kDa, ~35 kDa and ~ 45 kDa. Whole cell extracts (30 µg lysate) of K-562 (Lane 1), THP-1 (Lane 2), MCF7 (Lane 3) and IMR-32 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).