GPR30 Antibody PA5-28647 from Thermo Fisher Scientific

Product Specs
ItemGPR30 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-28647
Quantity100 µL
ApplicationsImmunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate/TagUnconjugated
FormatPBS with 20% glycerol and 0.025% Proclin 300; pH 7
ReactivityHuman, Mouse, Rat
ClonalityPolyclonal
TargetGPR30
IsotypeIgG
HostRabbit
NCBI Full Gene NameG protein-coupled estrogen receptor 1
NCBI Gene Aliases6330420K13Rik, Ceprl, GPER, CEPR, LyGPR, LERGU, LERGU2, Gper, GPR30, FEG-1, mER, GPCR-Br, Gpr30, DRY12, GPR41, CMKRL2
Concentration1.26 mg/mL
ImmunogenSynthetic peptide encompassing a sequence within the Intracellular domain of human GPR30. The exact sequence is proprietary
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

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Thermo Fisher Scientific

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Waltham, MA 02451

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Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cellsInt J Cardiol Heart VascMay 15, 2026Basant Elsaid, Irakli Kopaliani, Ansam Seif, Stephan Speier, Andreas Deussen1:250), ERβ (rabbit, Thermo Fisher Scientific, PA1-311, 1:1000), GPR30 (rabbit, Thermo Fisher Scientific, PA5-28647, 1:1000), and β2-AR (rabbit, Abcam, ab182136, 1:1000). Western blot analysis showed multiple non-specific

(2) Estrogen Receptor Expression Changes After Puberty in the Porcine Anterior Cruciate LigamentbioRxivMarch 14, 2026Jacob D. Thompson, Matthew B. Fisherreceptor alpha (1:1000 ERα, PA1–309 ThermoFisher), g-coupled protein estrogen receptor (1:800 GPR30, PA5–28647 ThermoFisher), and GAPDH (1:1000, 2118S Cell Signaling Technologies) overnight in 5% BSA at 4°C. Progesterone

(3) Effects of 17β-Estradiol Treatment on Metabolic Function and Aortic Relaxation in Castrated Male RatsInternational Journal of Molecular SciencesSeptember 12, 2025Rifat Ara Islam, Md Rahatullah Razan, Ankita Poojari, Mohammad Moshiur Rahman, Hao Wei, Hana S. Alhamadsheh, Melanie Felmlee, Atefeh Rabiee, Mitra Esfandiarei, Roshanak Rahimian(#ab3576) and Glut-4 (#ab654) were obtained from Abcam (Waltham, MA, USA) and antibodies against GPR30 (#PA5-28647) and iNOS (#PA1-036) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). 1:1000 dilution

(4) G-Protein-coupled Estrogen Receptor 1 (GPER1) Overexpression Affects Aggressiveness of Cervical Carcinoma Cells Depending on Histological EntityCancer Genomics ProteomicsApril 25, 2025Lena Hambach, Julia Gallwas, Carsten GründkerMerck Millipore, Darmstadt, Germany). Primary antibodies were used against GPER1 at 1:2,000 dilution (PA5-28647, Thermo Fisher, Waltham, MA, USA) and GAPDH at 1:2,000 dilution (5174S, Cell Signaling, Danvers, MA,

(5) N-(2-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Induces Apoptosis and Cell Cycle Arrest in Breast Cancer Cells, Decreasing GPER ExpressionMoleculesJuly 26, 2024Berenice Prestegui Martel, Alma Delia Chávez-Blanco, Guadalupe Domínguez-Gómez, Alfonso Dueñas González, Patricia Gaona-Aguas, Raúl Flores-Mejía, Selma Alin Somilleda-Ventura, Octavio Rodríguez-Cortes, Rocío Morales-Bárcena, Alberto Martínez Muñoz, et al.with antibodies against anti-β actin (1:10,000) (Sigma Aldrich, Merck KGaA) and anti-GPER (1:3000) (PA5-28647) (ThermoFisher, San Francisco, CA, USA) overnight at 4 °C. The secondary antibodies were diluted 1:1000

(6) G Protein-Coupled Estrogen Receptor (GPER) and ERs Are Modulated in the Testis-Epididymal Complex in the Normal and Cryptorchid DogVeterinary sciencesJanuary 5, 2024Liguori G, Tafuri S, Pelagalli A, Ali' S, Russo M, Mirabella N, Squillacioti C.Burlingame, CA, USA), sections were incubated with rabbit polyclonal anti-GPR30 antibody (dil. 1:500, cod. PA5-28647, Invitrogen, Waltham, MA, USA), mouse monoclonal anti-ESR1 (dil. 1:200, cod. TA807239, OriGene Technologies

(7) Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent mannerCell death discoveryOctober 17, 2023Snyder K, Gorse K, Kochanek PM, Jackson TC.Cat#19930-1-AP, Lot#00049598 (ProteinTech); Caspase-3, Cat#19677-1-AP Lot#00094596 and #00109139; GPR30, Cat#PA5-28647, Lot#YD3884109C (ThermoFisher); Estrogen Receptor α, Cat#ab76228, Lot#1016753-3 (Abcam). Secondary antibodies:

(8) Different Expression Pattern of G Protein-Coupled Estrogen Receptor GPER1 in Esophageal Squamous Cell Carcinoma and AdenocarcinomaInternational journal of molecular sciencesSeptember 13, 2023Liu J, Niu Y, Zhang B, Sun Q, Li H, Bai L, Su Z.for immunohistochemistry analysis of the tissue microarrays. The slides were stained with the GPER1 antibody (PA5-28647; Invitrogen, Carlsbad, CA, USA) [12,52,53] and visualized with DAB after incubation with the secondary

(9) Effect of Tamoxifen on Proteome Expression during In Vitro Myogenesis in Murine Skeletal Muscle C(2)C(12) CellsJournal of proteome researchSeptember 1, 2023Morris EA, Abreu A, Scordilis SP.(mouse),"ProteinTech, 60004-1","1:25,000" G-protein-coupled estrogen receptor,Polyclonal IgG (rabbit),"Invitrogen, PA5-28647",1:2500 superoxide dismutase 1,Monoclonal IgG2a (mouse),"ProteinTech, 67480-1",1:7500 superoxide dismutase

(10) Magel2 knockdown in hypothalamic POMC neurons innervating the medial amygdala reduces susceptibility to diet-induced obesityLife science allianceNovember 1, 2022Choi Y, Min HY, Hwang J, Jo YH.

(11) Suppression of G Protein-coupled Estrogen Receptor 1 (GPER1) Enhances the Anti-invasive Efficacy of Selective ERβ AgonistsAnticancer researchNovember 1, 2022Schmitz V, Bauerschmitz G, Gallwas J, Gründker C.

(12) Differential Effects of the G-Protein-Coupled Estrogen Receptor (GPER) on Rat Embryonic (E18) Hippocampal and Cortical NeuronseNeuroJuly 1, 2022Pemberton K, Rosato M, Dedert C, DeLeon C, Arnatt C, Xu F.Invitrogen, catalog #13-1500, neuronal marker) and polyclonal rabbit anti-GPER (Invitrogen, catalog #PA5-28647) antibodies diluted in blocking solution (1:200) and incubated overnight at 4°C. The primary antibodies

(13) Cytoplasmic G Protein-Coupled Estrogen Receptor 1 as a Prognostic Indicator of Breast Cancer: A Meta-AnalysisTechnology in cancer research & treatmentJanuary 1, 2022Zhang D, Wang J, Chen H, Yan S.(Abcam),Cytoplasm or nucleus,Given by author,No,Nucleus 0.92(0.68-1.25) Martin. et al,2018,700,UK,All,PA5-28647 (Thermo Scientific),Cytoplasm or nucleus,Cytoplasm：given by author. Nucleus: from survival curve.,Cytoplasm

(14) Estradiol/GPER affects the integrity of mammary duct-like structures in vitroScientific reportsJanuary 28, 2020Deng Y, Miki Y, Nakanishi A.for WB); laminin-5 (γ2 chain) mouse mAb (MAB19562, Merck, 1:500 for IF); GPER rabbit polyclonal antibody (PA5-28647, Thermo Fisher, 1:1000 for WB and 1:100 for IF and IHC); p38 MAPK (D13E1) rabbit mAb (#8690, Cell Signaling Technology

(15) Low expression of G protein-coupled oestrogen receptor 1 (GPER) is associated with adverse survival of breast cancer patientsOncotargetMay 25, 2018Martin SG, Lebot MN, Sukkarn B, Ball G, Green AR, Rakha EA, Ellis IO, Storr SJ.(TBS), and then treated with protein block solution. Rabbit polyclonal anti-GPER (Thermo Scientific (PA5-28647)) was used as primary antibody diluted 1:100 and was incubated on the tissue for one hour. Tissue was

(16) Knockdown of G Protein-coupled Estrogen Receptor 1 (GPER1) Enhances Tumor-supportive Properties in Cervical Carcinoma CellsCancer genomics & proteomicsJanuary 1, 0001"Ruckriegl S, Loris J, Wert K, Bauerschmitz G, Gallwas J, Gründker C."

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GPR30 Antibody from Thermo Fisher Scientific

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GPR30 Antibody from Thermo Fisher Scientific

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