LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2) MBS622333 from MyBioSource.com

Description

The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons

Product Specs
ItemLINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2)
CompanyMyBioSource.com
Price $635.00Supplier Page View Company Product Page
Catalog NumberMBS622333
ApplicationsWB, ELISA
Conjugate/TagUnconjugated
NCBI Full Gene NameLINE-1 type transposase domain containing 1
NCBI Gene AliasesECAT11
ReactivityHuman (Hu)
TargetLINE-1
ImmunogenRecognizes human LINE-1 at ~149kD
HostChicken; Source: Human
Molecular Weight~149kD
IsotypeIgY
ClonalityPolyclonal
PurityPurified
Purified by selective precipitation and salt fractionation followed by extensive dialysis
FormatSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
Concentration1mg/ml
Quantity0.1 mg

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Questions and Answers

Can this antibody be used to study the role of LINE-1 in cancer?

Yes, this antibody has been utilized in research to investigate the role of LINE-1 in cancer, particularly in relation to telomere maintenance in telomerase-positive tumor cells. Studies have shown that LINE-1 is critical for telomere maintenance, and its expression is associated with various cancers, making it a relevant target for cancer research.

What applications is this antibody suitable for?

The LINE-1 antibody is suitable for use in Western Blotting (WB) and Enzyme-Linked Immunosorbent Assay (ELISA). These applications allow for the detection and quantification of LINE-1 protein levels in various biological samples.

Is this antibody specific to human LINE-1, and how was it validated?

Yes, this antibody is specific to human LINE-1 and has been validated in studies that utilized polyclonal chicken anti-LINE-1 antibodies. The specificity is crucial for accurate detection in experiments focused on human tissues and cell lines.

What is the significance of LINE-1 expression in germ line tissues and certain cancers?

LINE-1 expression is tightly regulated and typically observed in germ line tissues, embryonic tissues, and specific cancers such as teratomas and testicular cancers. This regulation suggests that LINE-1 may play a role in developmental processes and cancer pathogenesis, highlighting its importance as a research target in understanding tumor biology.