Fig 1: Assessment of LSPC biomarkers. a Heterogeneity of gene expression in LSPCs. Co-expression analysis of representative gene pairs in E13.5 hepatoblasts are shown. b Definition of isolation sensitivity and specificity based on randomly selected single cells with cell type information inferred from global transcriptional profiles. c Sensitivity vs. specificity plot of 11 selected markers for E13.5 hepatoblasts. d Sensitivity vs. specificity plot of LSPC isolation using Cdh1 and Dlk1. e-f Co-expression analysis of E-cadherin, Anpep and Dlk1, and Dlk1 and Prom1 in E14.5 fetal livers via flow cytometry. Representative images from two replicative reactions for each condition are shown