Fig 1: Effects of LiCl and Res on mRNA expression levels in CDX2-overexpressing cells. Bar diagram shows the relative expression levels of RUNX3 (A), CDX2 (B), ß-catenin (C), TCF-4 (D), Bcl-2 (E), and Bax (F) mRNAs in MKN7 cells. Bar diagram shows the relative expression levels of RUNX3 (G), CDX2 (H), ß-catenin (I), TCF-4 (J), Bcl-2 (K), and Bax (L) mRNA in TMK1 cells. n = 3. Vs. CDX2 group, *p < 0.05, ??p < 0.01; vs. CDX2 + LiCl group, ##p < 0.01.
Fig 2: Protein expression levels in different treatment groups of cells. (A) Protein expression levels of RUNX3, CDX2, ß-catenin, TCF-4, Bcl-2, and Bax in MKN7 cells were determined using Western blotting. Bar diagram shows the relative expression levels of RUNX3 (B), CDX2 (C), ß-catenin (D), TCF-4 (E), Bcl-2 (F), and Bax (G) proteins in MKN7 cells. (H) Protein expression levels of RUNX3, CDX2, ß-catenin, TCF-4, Bcl-2, and Bax in TMK1 cells were determined using Western blotting. Bar diagram shows the relative expression levels of RUNX3 (I), CDX2 (J), ß-catenin (K), TCF-4 (L), Bcl-2 (M), and Bax (N) proteins in TMK1 cells. n = 3. Vs. Control group, *p < 0.05, ??p < 0.01; vs. NC group, #p < 0.05, ##p < 0.01; vs. CDX2 group, ^p < 0.05, ^^p < 0.01; vs. Res group, &p < 0.05, &&p < 0.01.
Fig 3: Relative mRNA expression levels in different treatment groups of cells. Bar diagram shows the relative expression levels of runt-related transcription factor 3 (RUNX3, A), CDX2 (B), ß-catenin (C), transcription factor (TCF)-4 (E), B-cell lymphoma-2 (Bcl-2, E), and Bcl-2-associated X protein (Bax, F) mRNA in MKN7 cells. Bar diagram shows the relative expression levels of RUNX3 (G), CDX2 (H), ß-catenin (I), TCF-4 (J), Bcl-2 (K), and Bax (L) mRNA in TMK1 cells. n = 3. Vs. Control group, *p < 0.05, ??p < 0.01; vs. NC group, #p < 0.05, ##p < 0.01; vs. CDX2 group, ^p < 0.05, ^^p < 0.01; vs. Res group, &p < 0.05, &&p < 0.01.
Fig 4: Immunohistochemistry of different proteins in different types of tissues. CDX2, RUNX3, ß-catenin, and TCF-4 expression in CAG (n = 34), GIM (n = 32), Dys (n = 38), and GC (n = 42) tissues. (Magnification × 100, scale bar 200 µm).
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