SP1 (D4C3) Rabbit mAb 9389S from Cell Signaling Technology

Product Specs
ItemSP1 (D4C3) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number9389S
Quantity100 µl
ApplicationsW, IP, IHC-P, FC-FP, ChIP, ChIP-seq, C&R
ReactivityH, Mk
ClonalityMonoclonal
TargetSP1
HostRabbit
NCBI Full Gene NameSp1 transcription factor
Research AreaChromatin Regulation / Nuclear Function
IsotypeIgG
CloneD4C3
Molecular Weight90

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Divergent roles of SPOP and CHD1 in ACSL4 regulation reveal context-dependent vulnerabilities for targeting ferroptosisNature CommunicationsJune 30, 2026Feiyu Chen, Qidong Li, Qianlin Gu, Javier Leo, Xin Liang, Naayaa Mehta, Estefania Labanca, Peter Shepherd, Iqbal Mahmud, Yin Wang, et al.overnight at 4 °C with antibodies against CHD1 (1:100, 4351S, Cell Signaling Technology) or SP1 (1:50, 9389S, Cell Signaling Technology). Antibody–protein complexes were captured using ChIP-Grade Protein G Magnetic

(2) Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical modelsCell Reports MedicineJune 16, 2026Yinan Wang, Qiongjie Zhou, Huanqiang Zhao, Zhilin Liu, Wei Li, Meng Xu, Jiangyuan Du, Yinfei Chen, Wei Cheng, Xinping Ng, et al.,Cell Signaling Technology,28692S; RRID:AB_2798962 USF1,proteintech,22327-1-AP; RRID:AB_2879073 SP1,Cell Signaling Technology,9389S; RRID:AB_11220235 ATF3,Affinity,DF3110; RRID:AB_2835487 EGR3,Solarbio,K008145P PINK 1,proteintech,23274-1-AP;

(3) Lnc00892 enhances cisplatin sensitivity by inducing apoptosis through the BTAF1/MDM2/STAT5B/XIAP axis in bladder cancer cellsBiology DirectMay 27, 2026Yixin Chang, Ning Sun, Yijie Liu, Zihui Jin, Peipei Zhang, Sijia Wang, Jie Chen, Jianting Liu, Zhenni Lin, Yongyong Lu, et al.caspase-3 (9661), Bax (5023), Bcl-2 (4223), survivin (2808,), c-IAP1 (7065), c-IAP2 (3130), HA (3724), SP1 (9389), MDM2 (86934), USP14 (11931), RXRα (3085) STAT4 (2653), STAT5 (94205) were obtained from Cell Signaling Technology

(4) A Metal‐Free Carbon Monoxide Prodrug Suppresses Metastasis of Pancreatic and Breast CancerAdvanced ScienceMarch 20, 2026Tiantian Zhang, Xiang Chen, Xiaoxiao Yang, Muskan Gori, Rohit Kumar Varshnaya, Dongning Liu, Cheryl Zhang, Michelle Yi Qin Chen, Zhengming Chen, George Zhang, et al.John's Laboratory, STJ196085), CYP1B1 (1:1,000, Abcam, 32649), SP1 (D4C3) (1:1,000, Cell Signaling, 9389), and β‐actin (1:1,000, Sigma, A2066) overnight, and then were incubated with horseradish peroxidase‐conjugated

(5) Exploring the mechanism of endothelial Pim-1 upregulation of tissue factor to initiate the hypercoagulable state in sepsisMolecular MedicineFebruary 12, 2026Qing Wang, Yun Hong, Ying Wang, Rongrong Zhang, Chen Zhou, Jingye Pan(Beijing, China). Anti-β-actin (4970), anti-GAPDH (5174), anti-mTOR (2983), anti-p-mTOR (5536), anti-Sp1 (9389) and anti-p-PRAS40 (Thr246) (C77D7) were acquired from Cell Signaling Technology, USA. Anti-Pim-1 (PA5-15123)

(6) DNA damage induced by HIV-1 Vpr triggers epigenetic remodeling and transcriptional programs to enhance virus transcription and latency reactivationPLoS BiolFebruary 2, 2026Nicholas Saladino, Emily Leavitt, Hoi Tong Wong, Jae-Hoon Ji, Diako Ebrahimi, Daniel J. SalamangoCell Signaling 91952T), Phospho-c-Jun (Ser73) (1:800, Cell Signaling 3270T), Sp1 (1:500, Cell Signaling 9389T), Phospho-Sp1 (Ser101) (1:300, Active Motif 39758), or S9.6 DNA-RNA hybrid (1:200, Kerafast ENH001)

(7) Arsenic promotes ROS-mediated malignant transformation of bronchial epithelial cells by specifically downregulating TXNL1 expressionCommunications BiologyNovember 22, 2025Lingling Zhao, Xueli Jiao, Hongyan Li, Yuanyuan Chang, Minghao Li, Yu Liu, Qipeng Xie, Chuanshu Huang, Haishan Huang(3724S, CST, 1:1000), anti-Myc-Tag (2276S, CST, 1:1000), anti-Ubiquitin (3933S, CST, 1:1000), anti-Sp1 (9389S, CST, 1:1000), anti-β-Actin (Ab0011, Abways Technology, 1:10000), anti-ETS-1 (sc-350, Santa Cruz, 1:500),

(8) Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3‐dependent PDGFB signalingCancer CommunicationsNovember 10, 2025Rui Tang, Yan Sun, Ao Deng, Jiahe Liu, Peijin Dai, Jing Chen, Chaoqun Deng, Hui Liu, Yuhang Hai, Yanran Tong, et al.4°C using 2 µg of antibody per sample, including anti‐H3K4me3 antibody (ab213224, Abcam), anti‐SP1 (#9389, Cell Signaling Technology) or control IgG (#2729, Cell Signaling Technology) with protein G beads.

(9) SP1‐Mediated Glycolytic Reprogramming Promotes Tumorigenesis and Progression in Pancreatic CancerAdvanced ScienceNovember 1, 2025Hexing Hang, Mengyu Yu, Linxi Zhu, Neng Tang, Xiao Fu, Zhenghua Cai, Minghao Yan, Yi Chen, Lei Yang, Jianzhuang Wu, et al.with specific primary antibodies, including β‐actin (AC026, ABclonal), VEGFA (A5708, ABclonal), SP1 (9389, CST), PFKFB4 (ab137785, Abcam) overnight at 4 °C. Afterward, the membranes were incubated with horseradish

(10) Atypical contribution of caspase-3 to melanoma cancer cell motility by regulation of coronin 1B activityCell Death DisOctober 6, 2025Kevin Berthenet, Serena Diazzi, Catherine Jamard, Kinga B. Stopa, Clara Gil, Stefan Dragan, Deborah Fanfone, Trang TM. Nguyen, Nathalie Al, Francois Virard, et al.Cell Signaling, 9662S; Euromedex, GTX110543), CASP7 (Cell Signaling, 12827S), SP1 (Cell Signaling, 9389S), CORO1B (Thermo Fisher Scientific, CF501573), P-CORO1B (Euromedex, EC-CP2621), GFP (Origene, TP401),

(11) Interplay of YEATS2 and GCDH regulates histone crotonylation and drives EMT in head and neck cancerElifeAugust 14, 2025Deepak Pant, Parik Kakani, Rushikesh Joshi, Abin Sabu, Shruti Agrawal, Atul Samaiya, Sanjeev Shuklathe Odyssey Membrane Scanning System. Antibodies used: YEATS2 (Proteintech, 24717–1-AP), SP1 (CST, 9389 S), SPARC (CST, 5420 S), GCDH (Sigma, HPA043252), N-Cadherin (Abcam, ab19348), Vimentin (Abcam, ab137321),

(12) The novel role of Kallistatin in linking metabolic syndromes and cognitive memory deterioration by inducing amyloid-β plaques accumulation and tau protein hyperphosphorylationeLifeAugust 5, 2025Qi W, Long Y, Li Z, Zhao Z, Shi J, Xie W, Wang L, Tan Y, Zhou T, Liang M, et al.Cell Signaling Technology, Boston, USA), PPARγ (#2435, Cell Signaling Technology, Boston, USA), SP1 (#9389, Cell Signaling Technology, Boston, USA), YY1 (#46395, Cell Signaling Technology, Boston, USA), Notch1

(13) Tet Methylcytosine Dioxygenase 3 Promotes Cardiovascular Senescence by DNA 5-Hydroxymethylcytosine-Mediated Sp1 Transcription Factor ExpressionMedComm (2020)June 19, 2025Yanqi Dang, Jing Ma, Shuang Ling, Shurong Wang, Huining Guo, Jun Liu, Guang Ji, Jin-Wen Xu2524, CST), TET2 (1:250, sc‐136926, Santa Cruz), TET3 (1:250, sc‐139186, Santa Cruz), SP1 (1:1000, 9389, CST), ETS‐1 (1:1000, 14069, CST), STAT3 (1:1000, 9139, CST), p‐STAT3 (1:1000, 9145, CST), β‐actin (1:5000,

(14) Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture methodNature biotechnologyMarch 1, 2025Li YR, Zhou Y, Yu J, Kim YJ, Li M, Lee D, Zhou K, Chen Y, Zhu Y, Wang YC, et al.IRF-1 (Clone D5E4, CST, cat. no. 8478, 1:1,000 dilution), antihuman SP1 (Clone D4C3, CST, cat. no. 9389, 1:1,000 dilution) and secondary antirabbit IgG (CST, cat. no. 7074, 1:1,000 dilution). β-Actin (Clone

(15) Topical TYK2 inhibitor ameliorates psoriasis-like dermatitis via the AKT-SP1-NGFR-AP1 pathway in keratinocytesClinical and translational medicineMarch 1, 2025Fang Z, Jiang R, Wang Y, Chen W, Chen X, Yin M.4511S, CST, 1:1000), Akt (cat. 4691S, CST, 1:1000), Phospho‐Akt (cat. 4056S, CST, 1:1000), SP1 (cat. 9389S, CST, 1:1000) and Phospho‐SP1(cat. ab59257, Abcam, 1:1000) were used as primary antibodies. HRP Goat

(16) Wnt signaling inhibits casein kinase 1α activity by modulating its interaction with protein phosphatase 2ACell ReportsFebruary 25, 2025Chen Shen, Wenhui Lu, Siva B. Merugu, Aradhana Bharti, Said M. Afify, Lauren Schnitkey, Daniel T. Wynn, Fan Yang, Thomas M. Rohwetter, Anmada Nayak, et al.,BD Biosciences,Cat# 610555; RRID: AB_397909 Anti PPP2CB,Abcam,Cat# ab168371 Anti SP1,Cell Signaling Technology,Cat# 9389; RRID: AB_11220235 Anti Axin1,Cell Signaling Technology,Cat# 2087; RRID: AB_2274550 Anti Axin1,MilliporeSigma,Cat#

(17) Transcriptional and post-transcriptional regulation of CARMN and its anti-tumor function in cervical cancer through autophagic flux blockade and MAPK cascade inhibitionJournal of Experimental & Clinical Cancer ResearchNovember 19, 2024Xing Zhang, Wenjing Yan, Hua Jin, Bingjia Yu, Hao Zhang, Bo Ding, Xue Chen, Yan Zhang, Qianqian Xia, Dan Meng, et al.(ChIP) ChIP was performed as previously reported [23] with a slight modification using antibody against SP1 (#9389, CST, USA), TFAP2α (#13019-3-AP, Proteintech, USA) or control IgG. RNA binding protein (RBP) immunoprecipitation assay

(18) SLC7A11 protects luminal A breast cancer cells against ferroptosis induced by CDK4/6 inhibitorsRedox biologyOctober 1, 2024Cui Y, Li Y, Xu Y, Liu X, Kang X, Zhu J, Long S, Han Y, Xue C, Sun Z, et al.primary antibodies: anti-β-Actin (1:2000, #3700S, Cell Signaling Technology, USA), anti-SP1 (1:1000, 9389S, Cell Signaling Technology, USA), anti-SLC7A11(1:1000, 12691S, Cell Signaling Technology, USA), anti-GPX4

(19) The Loss of an Orphan Nuclear Receptor NR2E3 Augments Wnt/β‐catenin Signaling via Epigenetic Dysregulation that Enhances Sp1‐β catenin‐p300 Interactions in Hepatocellular CarcinomaAdvanced ScienceAugust 1, 2024Yuet‐Kin Leung, Sung‐Gwon Lee, Jiang Wang, Ponmari Guruvaiah, Nancy J Rusch, Shuk‐Mei Ho, Chungoo Park, Kyounghyun Kimwas performed according to the manufacturer's protocol. For ChIP and Re‐CHIP assay, Sp1 (CST, cat # 9389) and β‐catenin (CST, cat # 8480) were used. The ChIP and Re‐ChIP primer set sequences used were: human

(20) HMGA1 promotes the progression of esophageal squamous cell carcinoma by elevating TKT-mediated upregulation of pentose phosphate pathwayCell Death & DiseaseJuly 30, 2024Meng-Jie Liu, Yuan Zhao, Qiu-Tong Li, Xin-Yuan Lei, Kai-Yue He, Jin-Rong Guo, Jing-Yu Yang, Zhen-Hua Yan, Dan-Hui Wu, Lei Zhang, et al.Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against cleaved caspase 3 (9661S) and SP1 (9389S) and rabbit IgG (#2729) were purchased from Cell Signaling Technology (Danvers, MA). Antibody against

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SP1 (D4C3) Rabbit mAb

SP1 (D4C3) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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SP1 (D4C3) Rabbit mAb from Cell Signaling Technology

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