Fig 1: Transferrin receptor two is a Negative Regulator of Human Erythropoiesis.(A) Quantitative RT-PCR and (B) Western blot showing the expression of TFR2 in human CD34+ cells five days post-infection with the respective lentiviral shRNAs targeting TFR2 (TFR2 sh1 and sh2) and a control luciferase gene (shLUC). (C) Representative FACS plots of erythroid cell surface markers CD71 (transferrin receptor) and CD235a (Glycophorin A) expression at various time points during erythroid differentiation. Percentages in each quadrant are represented as mean and standard deviation of 3 independent experiments (D) Hoechst staining showing more enucleated cells after TFR2 knockdown at day 21 of erythroid culture. (E) Representative histogram plots showing increased expression of CD235a (Glycophorin A) after TFR2 knockdown (F) Enhanced pSTAT5 response after TFR2 knockdown in UT7/EPO cells.