Fig 2: Tyrosination promotes EB1 and CLIP170 interactions on cardiomyocyte microtubules. a Representative AiryScan Joint Deconvoluted immunofluorescent images of EB1-CLIP170 PLA interactions in adult rat cardiomyocytes treated with null, TTL, or TTL-E331Q adenoviruses. b Representative western blot (top) and quantification (bottom) of EB1 in whole-cell lysate from adult rat cardiomyocytes treated with null, TTL, or E331Q adenoviruses for 48 h (N = 3 rat, n = 3 WB technical lanes). c Representative immunofluorescent images (left) and quantification (right) of CLIP170 in adult rat cardiomyocytes treated with null, TTL, or E331Q adenoviruses for 48 h (N = 3 rats, n = 10 cells per rat). d Quantification of EB1-CLIP170 PLA interactions in adult rat cardiomyocytes treated with null, TTL, or TTL-E331Q adenoviruses (N = 3 rats, n = 10 cells per rat). The bar represents mean ± 1SEM, and the middle line in the box graph represents mean ± 1SEM; statistical significance for (b) was determined with one-way ANOVA with post hoc test, and for (c) and (d) was determined with Kruskal–Wallis ANOVA with post hoc test

Fig 3: The CLIP170-IQGAP1 interaction is dependent upon anaphase astral microtubules.a Time-lapse images of GFP-CLIP170 during cell division. b Proximity ligation assays (PLA) mapping the proximity DIAPH1 to IQGAP1 (top), IQGAP1 to CLIP170 (middle) and CLIP170 to DIAPH1 (bottom) during mitotic progression. c Quantitation of PLA foci in metaphase and anaphase *p < 0.0001 using a nonparametric two-tailed Mann-Whitney t-tests for three experimental repeats, n = 50 cells examined over three independent experiments. d Proximity ligation assays (PLA) mapping the proximity DIAPH1 to IQGAP1 and IQGAP1 to CLIP170 in Centrinone B-treated cells. e Quantitation of PLA foci in d. At cell poles lacking astral microtubules, DIAPH1 and IQGAP1 are in close proximity but IQGAP1 and CLIP170 are not. *p = 0.0006, **p < 0.0001 using nonparametric two-tailed Mann-Whitney t-tests for three experimental repeats, n = 50 cells examined over three independent experiments. Scale bars are 10 µm.