CD73 (IE9) Antibody sc-32299 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemCD73 (IE9) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-32299
NCBI Gene AliasesCALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT
Antibody TypePrimary Antibodies
CloneIE9
Quantity200 µg/ml
Reactivityhuman
ApplicationsWB, IP, IF, IHC(P)
FormatPurified
ClonalityMonoclonal
Hostmouse
Conjugate/TagUnconjugated
NCBI Full Gene Name5'-nucleotidase ecto
TargetCD73
IsotypeIgG3

Questions and Answers

Can this antibody be used to study the role of CD73 in cancer progression?

Yes, the CD73 (IE9) Antibody has been utilized in studies investigating its role in various cancers, including pancreatic cancer, where it was found to be upregulated and correlated with poor prognosis. The antibody was used in immunohistochemical staining to assess CD73 expression in tumor tissues, highlighting its potential as a prognostic marker in cancer research .

Is this antibody effective for detecting CD73 in human tissues?

The CD73 (IE9) Antibody has been shown to effectively detect CD73 in human tissues through immunohistochemical analysis. It has been cited in studies assessing CD73 expression in oral squamous cell carcinoma and pancreatic ductal adenocarcinoma, confirming its utility in various tissue types .

How has this antibody been applied in stem cell research?

The CD73 (IE9) Antibody has been referenced in studies focusing on stem cell biology, particularly in the context of mesenchymal stem cell markers. It has been used to identify CD73 as a biomarker in the characterization of human corneal endothelial cells, which are relevant for stem cell therapies .

What experimental techniques can this antibody be used with?

The CD73 (IE9) Antibody is versatile and can be used in various applications, including Western blotting, immunofluorescence, immunohistochemistry, and immunoprecipitation. Its effectiveness in these techniques has been demonstrated in multiple studies, allowing for comprehensive analysis of CD73 in different experimental setups .

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Investigating the Role of TGF-β Signaling Pathways in Human Corneal Endothelial Cell Primary CultureCellsJune 14, 2023Aouimeur I, Sagnial T, Coulomb L, Maurin C, Thomas J, Forestier P, Ninotta S, Perrache C, Forest F, Gain P, et al.(05-369, Sigma-Aldrich). The three primary antibodies for the 3 biomarkers of EndMT were anti-CD73 (Sc-32299, Santa Cruz Biotechnology), anti-Col 5A1 (Sc-20648, Santa Cruz Biotechnology, Dallas, TX, USA), and

(2) The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid ModelCellsJanuary 16, 2023Zayat V, Kuczynska Z, Liput M, Metin E, Rzonca-Niewczas S, Smyk M, Mazurczak T, Goszczanska-Ciuchta A, Leszczynski P, Hoffman-Zacharska D, et al.Burlington, MA, USA, Cat# AB9260, RRID: AB_2142366" ,SOX1,,"Santa Cruz, Biotechnology, Dallas, TX, USA, Cat# sc-32299, RRID: AB_2033967" Differentiation markers,Goat anti-,01:10,"Thermo Fisher Scientific, Waltham, MA

(3) CD73 Inhibits cGAS-STING and Cooperates with CD39 to Promote Pancreatic CancerCancer immunology researchJanuary 3, 2023Jacoberger-Foissac C, Cousineau I, Bareche Y, Allard D, Chrobak P, Allard B, Pommey S, Messaoudi N, McNicoll Y, Soucy G, et al.room temperature, at 100 μL per well, undiluted. Detection antibody (anti-CD73 clone 1E9, Santa Cruz, sc-32299, 5 μg/mL) was incubated for 1 hour. HRP-conjugated anti-mouse IgG3 detection antibody (1:4000 dilution;

(4) Improving extracellular vesicles production through a Bayesian optimization-based experimental designEuropean journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.VJanuary 1, 2023Bader J, Narayanan H, Arosio P, Leroux JC.

(5) CD73 induces gemcitabine resistance in pancreatic ductal adenocarcinoma: A promising target with non-canonical mechanismsCancer lettersOctober 28, 2021Yu X, Liu W, Wang Z, Wang H, Liu J, Huang C, Zhao T, Wang X, Gao S, Ma Y, et al.

(6) Chip-Based Sensing of the Intercellular Transfer of Cell Surface Proteins: Regulation by the Metabolic StateBiomedicinesOctober 13, 2021Müller GA, Tschöp MH, Müller TD.sensing 1:1250; dot blotting 1:200]) was provided by Santa Cruz Biotechnology Inc., Heidelberg, Germany, (sc-32299). Anti-rCD73 antibody (rabbit polyclonal, affinity-purified, IgG isotype, prepared against recombinant

(7) Exosomes for Wound Healing: Purification Optimization and Identification of Bioactive ComponentsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)December 1, 2020Hettich BF, Ben-Yehuda Greenwald M, Werner S, Leroux JC.obtained from Avanti Polar Lipids (Alabaster, AL, USA). Primary mouse monoclonal antibodies anti‐CD73 (sc‐32299), anti‐CD63 (sc‐5275), anti‐CD9 (sc‐13118), anti‐TSG101 (sc‐7964), anti‐calregulin (sc‐373863), and

(8) Upregulation of CD73 Confers Acquired Radioresistance and is Required for Maintaining Irradiation-selected Pancreatic Cancer Cells in a Mesenchymal StateMolecular & cellular proteomics : MCPFebruary 1, 2020Nguyen AM, Zhou J, Sicairos B, Sonney S, Du Y.

(9) Prognostic value of CD73 expression in resected colorectal cancer liver metastasisOncoimmunologyJanuary 1, 2020Messaoudi N, Cousineau I, Arslanian E, Henault D, Stephen D, Vandenbroucke-Menu F, Dagenais M, Létourneau R, Plasse M, Roy A, et al.then incubated 2 h at 100 μl per well, undiluted. Detection antibody (anti-CD73 clone 1E9, Santa Cruz, sc-32299, 5 µg/ml) was incubated for 1 h. HRP-conjugated anti-mouse IgG3 detection antibody (1:4000 dilution;

(10) Tumor-Selective Altered Glycosylation and Functional Attenuation of CD73 in Human Hepatocellular CarcinomaHepatology communicationsOctober 1, 2019Alcedo KP, Guerrero A, Basrur V, Fu D, Richardson ML, McLane JS, Tsou CC, Nesvizhskii AI, Welling TH, Lebrilla CB, et al.

(11) The distinct role of CD73 in the progression of pancreatic cancerJournal of molecular medicine (Berlin, Germany)June 1, 2019Zhou L, Jia S, Chen Y, Wang W, Wu Z, Yu W, Zhang M, Ding G, Cao L.was carried out using the biotin-streptavidin method. The primary antibodies used were CD73 (1:100, sc-32299; Santa Cruz), tumor necrosis factor receptor (TNFR) 2 (1:100, ab109322; Abcam), and Ki-67 (1:250, ab16667;

(12) Mutual role of ecto-5'-nucleotidase/CD73 and concentrative nucleoside transporter 3 in the intestinal uptake of dAMPPloS oneJanuary 1, 2019Narumi K, Ohata T, Horiuchi Y, Satoh H, Furugen A, Kobayashi M, Iseki K.prepared and resolved using SDS-PAGE. Western blot was performed with antibodies against CD73 (Santa Cruz, sc32299, 1:1000), and β-actin (Millipore, MAB1501, 1/ 1000). (TIF) S3 Fig. Mutual role of CNT3 with CD73 in

(13) Verification of Long-Term Genetic Stability of hMSCs during Subculture after Internalization of Sunflower-Type Nanoparticles (SF-NPs)TheranosticsJanuary 1, 2018Park JS, Yi SW, Kim HJ, Oh HJ, Lee JS, Go M, Shim SH, Park KH.positive for surface markers, CD105 (sc51594, Ms, Santa Cruz), CD90 (sc53116, Ms, Santa Cruz), and CD73 (sc32299, Ms, Santa Cruz) of the cultured stem cells (Lot. #: 0000605220), and fewer than 10% were positive for

(14) Oxidative stress controls regulatory T cell apoptosis and suppressor activity and PD-L1-blockade resistance in tumorNature immunologyDecember 1, 2017Maj T, Wang W, Crespo J, Zhang H, Wang W, Wei S, Zhao L, Vatan L, Shao I, Szeliga W, et al.(clone 63828; R&D Systems), anti-mouse CD39 (clone 495826; R&D Systems), and anti-mouse CD73 (clone sc-32299; Santa Cruz Biotechnology). Proper secondary HRP-conjugated anti-rat, anti-rabbit (both from Vector

(15) CD73/NT5E is a target of miR-30a-5p and plays an important role in the pathogenesis of non-small cell lung cancerMolecular cancerFebruary 3, 2017Zhu J, Zeng Y, Li W, Qin H, Lei Z, Shen D, Gu D, Huang JA, Liu Z.

(16) The role of S100a4 (Mts1) in Apc- and Smad4-driven tumour onset and progressionEur J CancerNovember 1, 2016Atlasi Y, Noori R, Marolin I, Franken P, Brandao J, Biermann K, Collini P, Grigorian M, Lukanidin E, Ambartsumian N, et al.

(17) A study to identify and characterize the stem/progenitor cell in rabbit meniscusCytotechnologyOctober 1, 2016Huang H, Wang S, Gui J, Shen H.(1:1000; ab92574, Cambridge, MA, USA); and mouse antiCD73 (1:1000; Santa Cruz Biotechnology, Cat. #sc32299, Dallas, TX, USA) antibodies, respectively. Mouse anti-GAPDH (1:1000; Abcam, Cat. #ab9484, Cambridge,

(18) CD73 is associated with poor prognosis in HNSCCOncotargetSeptember 20, 2016Ren ZH, Lin CZ, Cao W, Yang R, Lu W, Liu ZQ, Chen YM, Yang X, Tian Z, Wang LZ, et al.

(19) Chordoma-derived cell line U-CH1-N recapitulates the biological properties of notochordal nucleus pulposus cellsJournal of orthopaedic research : official publication of the Orthopaedic Research SocietyAugust 1, 2016Fujita N, Suzuki S, Watanabe K, Ishii K, Watanabe R, Shimoda M, Takubo K, Tsuji T, Toyama Y, Miyamoto T, et al.

(20) CD73 as a novel marker for poor prognosis of oral squamous cell carcinomaOncology lettersJuly 1, 2016Ren ZH, Yuan YX, Ji T, Zhang CP.incubated overnight at 4°C (15 h) with primary mouse monoclonal anti-CD73 antibody (1:50 dilution; cat no. sc-32299; Santa Cruz Biotechnology, Inc., Dallas, TX, USA). After several washes with phosphate-buffered saline,

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CD73 (IE9) Antibody

CD73 (IE9) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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CD73 (IE9) Antibody from Santa Cruz Biotechnology, Inc.

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