Fig 1: CREPT is a target gene of miR-300. (A) A diagram of the potential target sites of miR-138 in the 3′-UTR of CREPT. (B) A luciferase reporter assay was performed using 293T cells co-transfected with CREPT 3′-UTR reporter vector and miR-300 mimic. *P<0.05 vs. scrambled control. The effect of transfection of miR-300 mimic on CREPT expression was evaluated by (C) RT-qPCR and (D) western blot analysis in HepG2 cells. The effect of transfection with miR-300 inhibitor on CREPT expression was evaluated by (E) RT-qPCR and (F) western blot analysis in Huh-7 cells. *P<0.05 vs. Blank. Scrambled control. CREPT, regulation of nuclear pre-mRNA domain-containing protein 1B; miR-300, microRNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; UTR, untranslated region; WT, wild-type; MT, mutant.