Periostin/OSF-2 Isoform 2 Antibody AF2955 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPeriostin/OSF-2 Isoform 2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2955
NCBI Gene AliasesOSF2, OSF-2, PDLPOSTN, Osf2, PLF, A630052E07Rik, PN
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Neutralization,Immunocytochemistry
IsotypeIgG
Conjugate/TagUnconjugated
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
TargetPeriostin/OSF-2
ReactivityHuman,Mouse,Rat,Transgenic Mouse
NCBI Full Gene Nameperiostin, osteoblast specific factor, periostin
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF-2, Asn24-Gln811, Accession # Q62009
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Periostin/OSF-2 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study periostin expression in cardiac fibroblasts during diabetic conditions?

Yes, the Periostin/OSF-2 Isoform 2 Antibody has been utilized in studies examining cardiac fibroblast activation in diabetic models. For instance, it was used in immunofluorescence assays to identify periostin expression in cardiac fibroblasts, demonstrating its role in the matrix-preserving phenotype associated with diabetes ().

Is this antibody effective for detecting periostin in breast cancer research?

The Periostin/OSF-2 Isoform 2 Antibody has been shown to be effective in breast cancer studies, where it was used to assess periostin expression in tumor cells. Research indicates that periostin expression is linked to a more aggressive cancer phenotype, highlighting its potential as a biomarker in breast cancer progression ().

What applications has this antibody been validated for in scientific research?

This antibody has been shown to work in various applications, including Western blotting, immunocytochemistry, and immunohistochemistry. It has been cited in multiple studies for its role in detecting periostin in different tissue types, including cardiac and tumor tissues (, ).

What species can this antibody detect periostin in?

The Periostin/OSF-2 Isoform 2 Antibody is reactive with mouse, human, and rat species, making it versatile for studies involving these organisms ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Creeping fat-derived mechanosensitive fibroblasts drive intestinal fibrosis in Crohn’s disease stricturesCellNovember 13, 2025Khristian E. Bauer-Rowe, Benjamin Pham, Michelle Griffin, Norah E. Liang, Alexia Kim, John M. Lu, Michael Januszyk, Jason L. Guo, Stefania De Santis, Yue Xing, et al.paraffin-embedded distal ilea from SAMP and AKR mice.87 Briefly, polyclonal goat anti-mouse POSTN (1:100, #AF2955, R&D Systems), monoclonal rabbit anti-mouse YAP (1:100, #14074, Cell Signaling Technology), and monoclonal

(2) Unraveling the development of cutaneous neurofibromas in neurofibromatosis type 1Acta Neuropathol CommunJuly 19, 2025Pernelle Pulh, Fanny Coulpier, Audrey Onfroy, Layna Oubrou, Wanzhen Zhang, Léa Toledano, Elie Abou Zougheib, Laura Fertitta, Pierre Wolkenstein, Piotr TopilkoAbcam #ab6640), rat anti-MBP (1:200; Chemicon Millipore #MAB386), goat anti-POSTN (1:400; BioTechne #AF2955), rabbit anti-TNC (1:100; Abcam #ab108930), rabbit anti-PGP9.5 (1:100; Abcam #ab108986), rabbit anti-nestin

(3) Inflammation-driven periostin in ECRS has contrasting effects on tissue structural integrity and osteitisFrontiers in ImmunologyJune 18, 2025Soo-In Kim, Min-Seok Rha, Jinsun Kim, Sang Hyeon Ahn, Ji-Hwan Ryu, Hyung-Ju Cho, Chang-Hoon Kimovernight at 4°C with primary antibodies against fibronectin (Abcam, ab2413), periostin (R&D Systems, AF2955), osteopontin (Abcam, ab218237), and α-SMA (Abcam, ab5694; all 1:200). After washing with TBST thrice

(4) PIEZO1 mediates periostin+ myofibroblast activation and pulmonary fibrosis in miceJ Clin InvestJune 2, 2025Liran Xu, Ting Li, Yapeng Cao, Yu He, Zehua Shao, Siyu Liu, Bianbian Wang, Ailing Su, Huijing Tian, Yongxin Li, et al.R&D), Pdgfr-β (1:100, Cat 14-1402-82, Invitrogen), Desmin (1:100, Cat 5332S, CST), Postn (1:100, Cat AF2955, R&D Systems), PIEZO1 (1:100, Cat 15939-1-AP, ProteinTech), Ki67 (1:100, Cat ab16667, Abcam), P21 (1:100,

(5) Alendronate partially rescues the periodontal defects in OIM mouse model of osteogenesis imperfectaScientific reportsJanuary 2, 2025Chen PJ, Wang K, Lin MH, Sharma R, Kalajzic Z, O'Brien M, Yadav S.(1:100, R&D Systems, Minneapolis, MN, AF1598), goat polyclonal anti-periostin (1:100, R&D Systems, AF2955), goat polyclonal anti-sclerostin (SOST, 1:100, R&D Systems, AF1589), mouse monoclonal anti-cathepsin-K

(6) CD34(+) PI16(+) fibroblast progenitors aggravate neointimal lesions of allograft arteries via CCL11/CCR3-PI3K/AKT pathwayTheranosticsJanuary 1, 2025Xu X, Zhu P, Wang H, Chen K, Liu L, Du L, Jiang L, Hu Y, Zhou X, Zhang B, et al.CD45 (R&D systems, AF114), Ki-67 (Abcam, ab16667), PDGFRa (R&D systems, AF1062), Postn (R&D systems, AF2955), Vimentin (Abcam, ab8978), PI16 (R&D systems, AF4929), CCL11 (R&D systems, AF-420-NA), and CCR3 (Proteintech,

(7) Single-nucleus transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regenerationeLifeDecember 6, 2024Perrin S, Ethel M, Bretegnier V, Goachet C, Wotawa CA, Luka M, Coulpier F, Masson C, Ménager M, Colnot C.polyclonal to mouse Osterix/Sp7 (ab22552, RRID:AB_2194492, Abcam), goat polyclonal to mouse Periostin (AF2955, RRID:AB_664123, R&D Systems), goat polyclonal to mouse PECAM1 (AF3628, RRID:AB_2161028, Bio-Techne),

(8) Periostin(+) myeloid cells improved long bone regeneration in a mechanosensitive mannerBone researchOctober 15, 2024Wang Z, Lin M, Pan Y, Liu Y, Yang C, Wu J, Wang Y, Yan B, Zhou J, Chen R, et al.(1:200; ab22552, Abcam, Cambridge, UK), F4/80 (1:200; ab6640, Abcam, Cambridge, UK), periostin (1:200; AF2955, R&D Systems, Minneapolis, MN, USA), CD31 Alex Fluor 488 (1:200; FAB3628G, R&D Systems, Minneapolis,

(9) Itm2a expression marks periosteal skeletal stem cells that contribute to bone fracture healingThe Journal of clinical investigationSeptember 3, 2024Xing W, Feng H, Jiang B, Gao B, Liu J, Xie Z, Zhang Y, Hu X, Sun J, Greenblatt MB, et al.(Abcam, ab15580, 1:200); rabbit anti-Runx2 (Abcam, ab114133, 1:200); mouse anti-Postn (R&D Systems, AF2955, 1:200); rat anti-PDGFRα (BioLegend, 135910,1:200); rabbit anti-Gli1 (Abcam, ab273018, 1:200); mouse

(10) Phenotypical and biochemical characterization of murine psoriasiform and fibrotic skin disease models in Stabilin-deficient miceFEBS open bioSeptember 1, 2024Krzistetzko J, Géraud C, Dormann C, Riedel A, Leibing T.Target,"Vendor, order number" TGFBi,"Abcam, ab170874, Cambridge, UK" Postn,"BioTechne, AF2955, Minneapolis, MN , USA" Antibody list.

(11) Single nuclei transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regenerationbioRxiv : the preprint server for biologyJuly 26, 2024Perrin S, Ethel M, Bretegnier V, Goachet C, Wotawa CA, Luka M, Coulpier F, Masson C, Ménager M, Colnot C.

(12) Spatial transcriptomics implicates impaired BMP signaling in NF1 fracture pseudarthrosis in murine and patient tissuesJCI InsightJuly 11, 2024Jonathan J. Rios, Conan Juan, John M. Shelton, Nandina Paria, Ila Oxendine, Meghan Wassell, Yared H. Kidane, Reuel Cornelia, Elise C. Jeffery, David A. Podeszwa, et al.stained overnight at 4°C in PBS containing 5% normal donkey serum with goat anti-periostin (1:100, AF2955, R&D Systems) and rabbit anti-osterix (1:200, ab22552, Abcam) antibodies. Slides were then stained for

(13) Deficiency of Stabilin-1 in the Context of Hepatic Melanoma MetastasisCancersJanuary 19, 2024Wohlfeil SA, Olsavszky A, Irkens AL, Häfele V, Dietsch B, Straub N, Goerdt S, Géraud C.Cambridge, UK), goat anti-Reelin (AF3820, R&D Systems, Minneapolis, MN, USA), goat anti-Periostin (AF2955, R&D Systems, Minneapolis, MN, USA), rabbit anti-TGFBI (ab170874, abcam, Cambridge, UK). Secondary antibodies:

(14) Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identityCell stem cellSeptember 7, 2023Ruscitto A, Chen P, Tosa I, Wang Z, Zhou G, Safina I, Wei R, Morel MM, Koch A, Forman M, et al.

(15) Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient MiceInternational journal of molecular sciencesJune 30, 2023Krzistetzko J, Géraud C, Dormann C, Riedel A, Leibing T.IF" Emcn,"ThermoFisher, 14-5851-85","IHC, IF" TGFBi,"Abcam, ab170874","IHC, IF, Simple Western" POSTN,"BioTechne, AF2955","IHC, IF, Simple Western" α-SMA,"Abcam, ab5694","IHC, IF" Desmin,"Abcam, ab15200","IHC, IF" F4/80,"BioLegend,

(16) Diabetes Induces Cardiac Fibroblast Activation, Promoting a Matrix-Preserving Nonmyofibroblast Phenotype, Without Stimulating Pericyte to Fibroblast ConversionJournal of the American Heart AssociationMarch 21, 2023Alex L, Tuleta I, Hanna A, Frangogiannis NG.Signaling Technology, Danvers, MA) and periostin staining (goat antiperiostin antibody, dilution 1:25, AF2955, R&D Systems, Minneapolis, MN) to identify cells with early characteristics of myofibroblasts (in the

(17) Periosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injurybioRxiv : the preprint server for biologyJanuary 13, 2023Marchand T, Akinnola KE, Takeishi S, Maryanovich M, Pinho S, Saint-Vanne J, Birbrair A, Lamy T, Tarte K, Frenette PS, et al.

(18) Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repairCell stem cellNovember 3, 2022Jeffery EC, Mann TLA, Pool JA, Zhao Z, Morrison SJ.

(19) Periostin gene expression in neu-positive breast cancer cells is regulated by a FGFR signaling cross talk with TGFβ/PI3K/AKT pathwaysBreast cancer research : BCRNovember 22, 2021Labrèche C, Cook DP, Abou-Hamad J, Pascoal J, Pryce BR, Al-Zahrani KN, Sabourin LA.XP,Cell Signaling Technology,5339 Goat Polyclonal Mouse Periostin/OSF-2 Isoform 2 Antibody,R&D Systems,AF2955 Goat Polyclonal Human Periostin/OSF-2 Antibody,R&D Systems,AF3548 Mouse anti-Goat IgG-HRP,Santa Cruz Biotechnology

(20) FGFR3 in Periosteal Cells Drives Cartilage-to-Bone Transformation in Bone RepairStem cell reportsOctober 13, 2020Julien A, Perrin S, Duchamp de Lageneste O, Carvalho C, Bensidhoum M, Legeai-Mallet L, Colnot C.(ab97959, 1:400, Abcam), SOX9 (ab182530, 1:1,000, Abcam), KI67 (ab15580, 1:200, Abcam), and POSTN (AF2955,1:400, BioTechne). Sections were washed and incubated with secondary antibody Alexa Fluor 488 goat anti-rabbit

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