GAD65 Antibody PA5-22260 from Thermo Fisher Scientific

Product Specs
ItemGAD65 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-22260
Quantity100 µL
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate/TagUnconjugated
ReactivityHuman, Mouse, Rat
TargetGAD65
IsotypeIgG
HostRabbit
NCBI Gene AliasesGad-2, gad65, GAD(65), GAD65, 6330404F12Rik
FormatPBS with 1% BSA, 20% glycerol and 0.025% Proclin 300; pH 7
ClonalityPolyclonal
NCBI Full Gene Nameglutamic acid decarboxylase 2, glutamate decarboxylase 2
Concentration0.25 mg/mL
ImmunogenRecombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

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Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Single-nucleus RNA sequencing reveals GABAergic vulnerability and reactive gliosis driven by loss of TDP-43iScienceNovember 21, 2025Rashmi Thapa, Navin Adhikari, Sishir Gautam, Mingming Sun, Sierra McOmie, Viyaleta Davydzenka, Derek Smith, Jack Syring, Harlie Kaligis, Jade Marie Kosmicki, et al.Antibodies Anti-Sst antibody,Invitrogen,Cat#PA5-85759;RRID:AB_2792896 Anti-Gad65 antibody,Invitrogen,Cat#PA5-22260;RRID:AB_11154107 Anti-TDP43 antibody,Proteintech,Cat#10782-2-AP;RRID:AB_615042 Horseradish peroxidase

(2) Deciphering pancreatic neuroendocrine tumors: Unveiling through circulating small extracellular vesiclesHeliyonApril 15, 2024Gorai PK, Rastogi S, Bharti PS, Agarwal S, Pal S, Sharma MC, Kumar R, Nikolajeff F, Kumar S, Rani N.(Invitrogen, PA5-17127), calnexin (E-AB-14819; Elabsciences), and anti-GAD65 (a marker for islets origin sEVs, PA5-22260, Invitrogen) antibodies. As the internal control, β-actin was used. The ImageJ program was used to quantify

(3) Generation of ventralized human thalamic organoids with thalamic reticular nucleusCell stem cellMay 4, 2023Kiral FR, Cakir B, Tanaka Y, Kim J, Yang WS, Wehbe F, Kang YJ, Zhong M, Sancer G, Lee SH, et al.PA5-32241 vGLUT2,Millipore,Cat# MAB5504 GAD1/GAD67,Invitrogen,Cat# MA5-24909 GAD2/GAD65,Invitrogen,Cat# PA5-22260 ECEL1,abcam,Cat# ab234710 SPP1,Invitrogen,Cat# MA5-17180 PTCHD1,Invitrogen,Cat# PA5-48191 PTCHD1,abcam,Cat#

(4) Epidural electrical stimulation of the cervical spinal cord opposes opioid-induced respiratory depressionThe Journal of physiologyJune 1, 2022Huang R, Worrell J, Garner E, Wang S, Homsey T, Xu B, Galer EL, Zhou Y, Tavakol S, Daneshvar M, et al.

(5) Ednrb (-/-) mice with hirschsprung disease are missing Gad2-expressing enteric neurons in the ganglionated small intestineFrontiers in cell and developmental biologyJanuary 1, 2022Bhave S, Guyer RA, Picard N, Omer M, Hotta R, Goldstein AM.lab), rabbit anti-MATH2/NEUROD6 (5 μg/ml, Abcam ab85824), rabbit anti-GAD65/GAD2 (1:100, Invitrogen PA5-22260), rabbit anti-nNOS (1:400, Invitrogen 61-7000), and rabbit anti-Calretinin (1:200, Invitrogen 18-0211).

(6) Input-specific modulation of murine nucleus accumbens differentially regulates hedonic feedingNature communicationsApril 9, 2021Christoffel DJ, Walsh JJ, Heifets BD, Hoerbelt P, Neuner S, Sun G, Ravikumar VK, Wu H, Halpern CH, Malenka RC.(Synaptic Systems, 226003), rat anti-mCherry 1:1500 (Invitrogen, M11217), rabbit anti-GAD65 1:1000 (PA5-22260), and donkey anti-rabbit, chicken, or rat secondary corresponding antibodies 1:200 (Alexa Fluor 488,

(7) Lithium treatment reverses irradiation-induced changes in rodent neural progenitors and rescues cognitionMolecular psychiatryJanuary 1, 2021Zanni G, Goto S, Fragopoulou AF, Gaudenzi G, Naidoo V, Di Martino E, Levy G, Dominguez CA, Dethlefsen O, Cedazo-Minguez A, et al.according to manufacturer’s instructions. Samples were denatured at 95 °C for 5 min. Rabbit α-GAD65 (PA5-22260, Thermo Fisher Scientific, USA) and Rabbit α-Tppp (ab92305, Abcam, USA) were used at a concentration

(8) Myricetin attenuates the severity of seizures and neuroapoptosis in pentylenetetrazole kindled mice by regulating the of BDNF-TrkB signaling pathway and modulating matrix metalloproteinase-9 and GABA(A)Experimental and therapeutic medicineApril 1, 2019Sun ZQ, Meng FH, Tu LX, Sun L.Scientific, Inc.), MMP‑9 (cat. no. 3852; 1:1,000; Cell Signaling Technology, Inc.) and GAD65 (cat. no. PA5‑22260; 1:2,000; Invitrogen; Thermo Fisher Scientific, Inc.) were obtained and Santa Cruz Biotechnology, Inc.

(9) GABAergic signaling linked to autophagy enhances host protection against intracellular bacterial infectionsNature communicationsOctober 10, 2018Kim JK, Kim YS, Lee HM, Jin HS, Neupane C, Kim S, Lee SH, Min JJ, Sasai M, Jeong JH, et al.568-conjugated anti-mouse IgG (1:400 diluted; A11004) were purchased from Invitrogen. Anti-GAD65 (1:400 diluted; PA5-22260) was purchased from Thermo Fisher Scientific. Muscimol (M1523), isoguvacine hydrochloride (G002), GABA

(10) Increased Frataxin Expression Induced in Friedreich Ataxia Cells by Platinum TALE-VP64s or Platinum TALE-SunTagMolecular therapy. Nucleic acidsSeptember 7, 2018Cherif K, Gérard C, Rousseau J, Ouellet DL, Chapdelaine P, Tremblay JP.MAB374, Etobicoke, ON, Canada), and anti-PGC-1α and anti-GAD65 (Thermo Fisher Scientific PA5-38022 and PA5-22260, Rockford, IL, USA). The secondary antibody used was a rabbit anti-mouse and anti-rabbit (Jackson ImmunoResearch,

(11) Anatomical and Electrophysiological Clustering of Superficial Medial Entorhinal Cortex InterneuronseNeuroSeptember 1, 2017Martínez JJ, Rahsepar B, White JA.GAD2-TdTomato and PV-Tdtomato animals were, respectively, stained with markers for GAD2 (Invitrogen, PA5-22260) and PV (Swant, PV25) followed by Alexa Fluor 488 goat-antirabbit secondary antibody. Imaging was performed

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GAD65 Antibody

GAD65 Antibody from Thermo Fisher Scientific

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GAD65 Antibody from Thermo Fisher Scientific

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