Immunofluorescence analysis of RPA2 was performed using 70% confluent log phase HeLa cells treated with 30 µmol/L Etoposide for 1 hour. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RPA2 Polyclonal Antibody (product # PA5-22256) at 5µg/mL in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear speckles upon Etoposide treatment. Panel e shows untreated cells without any staining. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

RPA2 Polyclonal Antibody detects RPA2 protein by Western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract.12 % SDS-PAGE. RPA2 Polyclonal Antibody (Product # PA5-22256) dilution: 1:1,000.