Fig 1: SOX9 regulates the invasion and migration of A549 cells by activating the RAP1 signaling pathway. A-B, RT-qPCR to test the expression levels of SOX9 and RAP1 in cells of each group; C-D, Western blot to detect the expression levels of RAP1, RAP1GAP and RasGRP3 in cells; E, Transwell to observe cell invasion levels; F, Scratch migration assay to observe the migration ability of cells. **P < 0.01, vs. sh-NC; ##P < 0.01, vs. sh-SOX9; ¡÷¡÷P < 0.01, vs. RAP1.
Fig 2: Up-regulating SOX9 in lung adenocarcinoma activates the RAP1 signaling pathway. A, RT-qPCR to check the expression of RAP1 in clinical samples; **P < 0.01; B, Pearson analysis for the correlation between SOX9 and RAP1 expression in clinical samples; C, CO-IP is used to analyze the interaction between RAP1 and SOX9. D-E, RT-qPCR to measure the expression of RAP1 in BEAS-2B cells, A549 cells (D) **P < 0.01;, and different expression levels of SOX9 cells (E);**P < 0.01, vs. sh-NC; ##P < 0.01, vs. Vector; F, Western blot to detect the expression levels of RAP1, RAP1GAP and RasGRP3 in each group of cells. **P < 0.01, vs. sh-NC; ##P < 0.01, vs. sh-SOX9.
Supplier Page from Abcam for Anti-RASGRP3 (phospho T133) antibody [EPNCIR148]