CD31/PECAM-1 Antibody NB100-2284 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemCD31/PECAM-1 Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberNB100-2284
ImmunogenThe immunogen recognized by this CD31/PECAM-1 Antibody maps to a region between residue 700 and the C-terminus (residue 738) of human CD31 using the numbering given in entry NP_000433.2 (Gene ID 5175)
Concentration0.1 mg/ml
TargetCD31/PECAM-1
HostRabbit
ReactivityCanine,Human,Mammal,Mouse,Porcine,Rat
Quantity0.1 ml
FormatTBS, 0.1% BSA
ApplicationsIF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
ClonalityPolyclonal
PurityImmunogen affinity purified
IsotypeIgG
Molecular Weight82.5 kDa
Research AreaAngiogenesis, Cancer, Cytoskeleton Markers, Embryonic Stem Cell Markers, Endothelial Cell Markers, Extracellular Matrix, Hematopoietic Stem Cell Markers, Immunology, Myeloid Cell Markers, Signal Transduction, Stem Cell Markers
Antibody TypePrimary Antibodies
StorageStore at 4C. Do not freeze
NCBI Full Gene Nameplatelet and endothelial cell adhesion molecule 1
NCBI Gene AliasesCD31/EndoCAM, PECAM-1, CD31, PECA1, GPIIA', endoCAM

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 ml

Questions and Answers

What species can this CD31/PECAM-1 antibody be used on for immunohistochemistry?

The CD31/PECAM-1 antibody has been shown to work in immunohistochemistry on various species including human, mouse, rat, canine, bovine, feline, horse, non-human primate, goat, hamster, guinea pig, porcine, donkey, rabbit, and sheep.

Has this antibody been utilized in any specific studies related to cancer or angiogenesis?

Yes, the CD31/PECAM-1 antibody has been used in studies investigating angiogenesis and tumor interactions. For instance, it was employed in a study examining the response of the neurovascular unit to brain metastatic breast cancer cells, highlighting its role in understanding tumor cell migration and endothelial interactions (Haskó et al., 2019).

Can this antibody be used in flow cytometry applications?

Yes, the CD31/PECAM-1 antibody has been documented for use in flow cytometry, as indicated in various studies that assess endothelial cell populations and their interactions in different biological contexts.

What is the recommended dilution for using this antibody in Western blotting?

The recommended dilution for the CD31/PECAM-1 antibody in Western blotting is 1:1000, as noted in comparative studies that utilize this antibody for detecting CD31 expression in various experimental setups.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Animal-free matrix alternative for three-dimensional in vitro angiogenesis modelsFrontiers in ToxicologyJune 18, 2026Elle Koivunotko, Chris S. Pridgeon, Lauri Paasonen, Riina Harjumäkiwere incubated overnight at 4 °C with the primary antibodies (anti-rabbit CD31/PECAM (1:200; Cat. #NB100-2284), anti-rabbit vWF (1:200, #NBP1-84761) and anti-mouse NG2/MCSP (1:200, Cat. #NBP2-80873) all Novus Biologicals,

(2) Human CD24+ dental papilla cells are competent seed cells for dentin-pulp regeneration via BMP2/SIRT1 axisNature CommunicationsJune 12, 2026Cheng Liang, Zhi Liu, Jie Li, Cong Han, Yu Gao, Bohuai Zhou, Qiuyu Chen, Fei Bi, Chengcheng Liao, Weimin Wu, et al.Signaling Technology), LC3 (1:300, 14600-1-AP, Proteintech), SOD1 (1:300, ab51254, Abcam), and CD31 (1:300, NB100-2284, Novus Bio). After DPBS washes, fluorescence-conjugated secondary antibodies (1:1000, Invitrogen) were

(3) Identification of the adhesion GPCR ADGRL4/ELTD1 as a novel potential prognostic biomarker for cerebral cavernous malformation diseaseMolecular MedicineMay 8, 2026Andrea Perrelli, Ashraqat Ahmed, Anna Onisiforou, Gwenola Boulday, Juan Zalvide, Celia M. Pombo, José E. Martínez, Alessio Paolini, Salim Abdelilah-Seyfried, Ioannis Petrakakis, et al.(ab150489, dilution 1:400, Abcam, Cambridge, UK), and with polyclonal anti-human CD31/PECAM-1 antibody (NB100—2284, dilution 1:400, Novus Biologicals, Centennial, CO, USA). The primary antibodies were diluted in Dako

(4) Spatial associations between neuronal membrane damage and vasculature after repetitive diffuse TBI in pigsActa Neuropathologica CommunicationsMay 5, 2026Kathryn L. Wofford, Erin M. Purvis Conway, Victor P. Acero, Jerry Gao, Olivia M. Rivellini, Emory Kuo, Constance J. Mietus, Kevin D. Browne, John C. O’Donnell, D. Kacy Cullen(αSMA; 1:500, mouse, Abcam; #ab7817), a marker of pericytes; CD31 (1:100, rabbit, Novus Biologicals; #NB100-2284), a marker of endothelial cells; glial-fibrillary acidic protein (GFAP; 1:500, rabbit, Millipore; #AB5804),

(5) Vitrification versus slow freezing for prepubertal ovarian tissue cryopreservation: insights from a porcine xenotransplantation modelJournal of Ovarian ResearchApril 17, 2026Yingchun Guo, Xiaoping Liu, Jianhong Chen, Yun Xie, Wenlong Su, Peng Sun, Haitao Zeng, Cong Fang, Xiaoyan Liangwith the primary antibody (VEGFA, 1:400, NB100-664, Novus Biologicals, Colorado, USA; CD31, 1:200, NB100-2284, Novus Biologicals, Colorado, USA) overnight at 4 °C. After being washed with phosphate-buffered saline

(6) Spermidine Attenuates Neuroimmune Dysfunction in Gulf War Illness via Modulation of the Gut- Brain AxisMol NeurobiolApril 10, 2026Ayushi Trivedi, Subhajit Roy, Madhura More, Dipro Bose, Punnag Saha, Ram Kumar, Subham Sarkar, Jonathan Skupsky, Ashok Tuteja, Kimberly Sullivan, et al.(1:300; AB216327), anti-claudin-2 (1:250; AB53032) from Abcam (Cambridge, MA, USA), anti-CD-31 (1:250; NB100-2284, Novus Biologicals, Centennial, CO, USA), anti-claudin-5 (1:250; sc-374221), anti-RAGE (1:250; sc-365154)

(7) Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous systemBrain Struct FunctApril 4, 2026Lukáš Malčický, Ján Košuth, Daniel Barčák, Jarmila Zrubáková, Marie Vancová, Zuzana Daxnerová, Juraj Ševc, Anna Alexovič Matiašová(IF) Anti-CD31/PECAM-1,Rabbit polyclonal,Human CD31 aa 700–738 (P16284) (intracellular region),NOVUS BIOLOGICALS,NB100-2284,AB_10002513,1:100 (IF) CD31 (PECAM-1) monoclonal (clone 390),Rat monoclonal,"N/A, reacts with mouse

(8) Towards a standardized diabetic prolonged wound healing model in hairless SKH1 miceExperimental Biology and MedicineMarch 20, 2026Elle Koivunotko, Julia Monola, Chris S. Pridgeon, Jere Linden, Riina Harjumäki, Emrah Yatkin, Mari Madetoja, Marjo Yliperttulaat 4 °C with anti-rabbit CD31/platelet endothelial cell adhesion molecule (CD31/PECAM-1, 1:100, CAT: NB100–2284, Novus Biologicals, UK) or 1 h at room temperature with anti-rabbit ionized calcium-binding adaptor

(9) Connexin 43 and Pannexin 1 in Renal Cell Populations in Diabetic Kidney DiseaseInternational Journal of Molecular SciencesFebruary 25, 2026Marinela Jelinčić Korčulanin, Anita Racetin, Nikola Pavlović, Ivo Jeličić, Merica Glavina Durdov, Monika Andrzejewska, Leo Jerčić, Ivana Bočina, Nives Kević, Ivana Restović, et al.(JB51-33),NBP2-75624,Rabbit,1:100,"Novus Biologicals, Centennial, CO, USA" Primary,Anti-CD31/PECAM-1 Antibody,NB100-2284,Rabbit,1:100,"Novus Biologicals, Centennial, CO, USA" Primary,Anti-PECAM-1 Antibody,Sc-53389,Mouse,1:50,"Santa Cruz Biotechnology Inc

(10) Pulmonary Vascular Proliferative Lesions in Wild Korean Raccoon Dogs (Nyctereutes procyonoides): Description of 13 CasesVeterinary SciencesDecember 24, 2025Warisraporn Tangchang, Jun-Yeop Song, Do-hyun Kim, Hyo-Jung Kwon, Hwa-Young SonAntibody,Type 1,Clonality 2,Clone or ID,Species,Source of Antibody,Dilution,Expression Area CD31,E,pAb,NB100-2284,rabbit,Novus Biologicals,1:100,Cytoplasm Cytoplasm,vWF,E,pAb,ab6994,rabbit,abcam,1:200 Cytoplasm,α-SMA,F,mAb,NBP2-33006,mouse,Novus Biologicals

(11) Glutathione peroxidase mimic-engineered α-lactalbumin self-assembling coatings tailor immunomodulatory vascular stents for suppressing restenosisBioact MaterDecember 3, 2025Yan Fu, Nan Lyu, Xia Tang, Ying Wang, Xiaohui Mou, Wentai Zhang, Siwei Yin, Mu Li, Na Bai, Nan Huang, et al.staining was then performed sequentially: samples were incubated with CD31/PECAM-1 Antibody (Novus, Cat# NB100-2284, RRID:AB_10002513, 1:300) for 2 h, followed by incubation with Alexa Fluor® 488 (Thermo Fisher Scientific,

(12) Geometrical Designs in Volumetric Bioprinting to Study Cellular Behaviors in Engineered ConstructsAdvanced Healthcare MaterialsNovember 3, 2025Julia Simińska‐Stanny, Pierre Tournier, Armin Shavandi, Shukry J Habibadded to stain nuclei. For immunostaining, primary Rabbit antibody anti‐CD31, 1:500 (Novus Biologicals, NB100‐2284) for HUVECs and Rabbit anti‐H3K27me3 1:500 (Thermo Fisher Scientific, MA5‐11198) for 143b cells were

(13) Lipid raft proteomics identify endothelial myosin-9 (MYH9) as a regulator of low-density lipoprotein transcytosis and atherosclerosisProceedings of the National Academy of SciencesOctober 28, 2025Erika Jang, Siavash Ghaffari, Andria Henry, Grace Wen, Tse Wing Winnie Ho, Wan Hei Cheng, Yunfan Lyu, Kuiru Wei, Changsen Wang, Casey E. Romanoski, et al.min, then washed in PBS. Aortas were incubated in 1 µg/mL rabbit anti-mouse CD31 primary antibody (NB100-2284; Novus) on a shaker overnight at 4 °C. After washing, 3.75 µg/mL Alexa Fluor 488 secondary antibody

(14) One-step strategy for fabricating icariin-encapsulated biomimetic Scaffold: Orchestrating immune, angiogenic, and osteogenic cascade for enhanced bone regenerationBioact MaterJune 10, 2025Fengxin Zhao, Fuying Chen, Tao Song, Luoqiang Tian, Hang Guo, Dongxiao Li, Jirong Yang, Kai Zhang, Yumei Xiao, Xingdong ZhangAbcam, UK) were performed to further verify the phenotype of the macrophages. The IF staining of CD31 (NB100-2284, Novus, USA) and α-SMA (GB111364-100, Servicebio, China) were performed to observe angiogenesis. The

(15) The effect of the foreign body response on drug elution from subdermal delivery systemsBiomaterialsJune 1, 2025Simone Capuani, Nathanael Hernandez, Jocelyn Nikita Campa-Carranza, Nicola Di Trani, Takuma Yoshikawa, Marco Farina, Ashley L. Joubert, Camden A. Caffey, Alessio Simeone, Seo Won Won Cho, et al.was segmented manually. Antibodies used were: anti-CD45 (ab10558, Abcam, 2 μg/ml), anti-CD31/PECAM-1 (NB100–2284, Novus Bio, 0.5 μg/ml), AlexaFluor 555 goat anti-rabbit (A21428, Invitrogen, 4 μg/ml). 2.9. Collagen

(16) Clinically relevant clot resolution via a thromboinflammation-on-a-chipNatureMay 29, 2025Yongzhi Qiu, Jessica Lin, Audrey Wang, Zhou Fang, Yumiko Sakurai, Hyoann Choi, Evelyn K. Williams, Elaissa T. Hardy, Kristin Maher, Ahmet F. Coskun, et al.1xPBS, and then blotted with 1% BSA (Sigma, Cat#A2153). The PECAM1 (1:100, Novus Biologicals, Cat# NB100–2284) and VE-cadherin (1:100, Santa Cruz Biotechnology, Cat# sc-9989) antibodies in 1xPBS containing 1% BSA

(17) Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet TransplantationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2025Campa-Carranza JN, Capuani S, Joubert AL, Hernandez N, Bo T, Sauceda-Villanueva OI, Conte M, Franco L, Farina M, Rome GE, et al.blocking with 5% goat serum in 0.1% BSA/TBS, sections were incubated with primary antibodies CD31 (NB100‐2284, Novus Biologicals, 1:200), VE Cadherin (36‐1900, Invitrogen, 1:25), and eNOS (ab300071, Abcam, 1:50)

(18) Cytomegalovirus host receptor expression in the human fetal inner earPLoS OneMarch 31, 2025Lucia C. M. Grijpink, Wouter H. van der Valk, Edward S. A. van Beelen, John C. M. J. de Groot, Heiko Locher, Ann C. T. M. Vossen,Santa Cruz Biotechnology,sc-13117,AB_628044,1:200 PDGFRA,Goat,Novus Biologicals,AF-307-NA,AB_354459,1:100 PECAM1,Rabbit,Novus Biologicals,NB100-2284,AB_10002513,1:100 TUBB3,Mouse,Abcam,ab7751,AB_306045,1:500 TUBB3,Rabbit,Abcam,ab18207,AB_444319,1:200 ,Isotype

(19) Freeze-Dried Porous Collagen Scaffolds for the Repair of Volumetric Muscle Loss InjuriesACS Biomaterials Science & EngineeringMarch 10, 2025Ivan M. Basurto, Geshani C. Bandara, Ryann D. Boudreau, Sydney B. Shriver, Samir A. Muhammad, George J. Christ, Steven R. CaliariAbcam), CD68 (1:100, MCA341R; Bio-Rad) and CD163 (1:400, ab182422; Abcam), CD31 (1:250; Novus Biologicals NB100–2284) and α-smooth muscle actin (αSMA, conjugated to 488 fluorochrome, F3777, 1:250; Sigma-Aldrich), or neurofilament

(20) Cell-scale porosity minimizes foreign body reaction and promotes innervated myofiber formation after volumetric muscle lossNPJ Regen MedMarch 1, 2025Areli Rodriguez Ayala, George Christ, Donald GriffinPrimary antibody,Company,Catalog number,Dilution CD31,Novus Biologicals,NB100-2284,1:200 CD68,Bio-Rad,MCA341R,1:100 CD163,Abcam,ab182422,1:200 NF200,Invitrogen,PA1-10016,1:200 MF20,DSHB,MF

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