Fig 1: SLCO3A1 promotes follicular growth in mice. The mRNA and protein levels of SLCO3A1 in the ovaries of mice treated with LV-SLCO3A1 (A) and sh-SLCO3A1 (B). (C) The TUNEL staining was performed in mice ovaries treated with LV-SLCO3A1 or sh-SLCO3A1, and the relative fluorescence intensity was corrected by that of the control groups. Yellow arrows indicated apoptotic GCs. Scale bar = 500 μm. (D) The HE staining and follicular statistics of mice after their ovaries were treated with LV-SLCO3A1 or sh-SLCO3A1. Black arrows indicated antral follicles, and orange arrows indicated corpus luteum. Scale bar = 500 μm. (E) The age of mice at vaginal opening treated with LV-SLCO3A1 or sh-SLCO3A1. (F) The concentration of E2 treated with LV-SLCO3A1 or sh-SLCO3A1 was assessed by ELISA. RT-qPCR analysis of proliferation- and cycle-related genes expression treated with LV-SLCO3A1 (G) or sh-SLCO3A1 (I). Western blot analysis of CCNB2 and CDK1 treated with LV-SLCO3A1 (H) or sh-SLCO3A1 (J). * p < 0.05, ** p < 0.01, *** p < 0.001.