GAPDH (1D4) Antibody sc-59540 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemGAPDH (1D4) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-59540
Antibody TypePrimary Antibodies
NCBI Gene AliasesGapd, GAPD, HEL-S-162eP, G3PD
Quantity100 µg/ml
Clone1D4
ApplicationsWB, IP, IF
Reactivitymouse, rat, human, bovine, porcine
ClonalityMonoclonal
Hostmouse
Conjugate/TagUnconjugated
FormatPurified
NCBI Full Gene Nameglyceraldehyde-3-phosphate dehydrogenase
TargetGAPDH
IsotypeIgM

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) BET inhibition curbs macrophage inflammation, lipid accumulation, and atherogenesis by disrupting the YAP/TAZ-BRD4 axisJournal of Leukocyte BiologyJuly 3, 2026Praveen Krishna Veerasubramanian, Vijaykumar S Meli, Hamza Atcha, Wenqi Wang, Timothy L Downing, Wendy F Liu14347S), or anti-OLR1 (1:1,000, Abcam Cat. ab60178) antibodies. GAPDH antibody (1:2,000, Santa Cruz, Cat. sc-59540) was used to normalize the cell lysate loading. After 3 × washes using TBST, secondary antibodies conjugated

(2) Methylglyoxal Accumulation is Associated with Brain Inflammation after Myocardial Infarction with Sex and Regional DifferencesAdvanced ScienceApril 9, 2026Ramis Ileri, Xixi Guo, Erik J. Suuronenat 1:1000 dilution), anti‐claudin 5 (Invitrogen, Cat#34‐1600 at 1:500), anti‐GAPDH (Santa Cruz, Cat#sc‐59540 at 1:1000 dilution) and anti‐α‐tubulin (Cell Signaling, Rabbit, Cat#2125 at 1:1000 dilution) primary

(3) Integrated transcriptome and proteome analyses unveil cytoskeletal alterations in an endothelial model of monogenic diabetesGenome MedicineFebruary 27, 2026Dawid Skoczek, Damian Kloska, Marta Targosz-Korecka, Krzysztof Szade, Artur Biela, Jerzy Hohendorff, Marian Babincak, Aleksandra Kopacz, Maciej T. Malecki, Jacek Stepniewski, et al.overnight with primary antibodies against HNF1A (Abclonal AB3092), GAPDH (Santa Cruz Biotechnology sc-59540) at 4° C. Then they were washed with 0.1% Tween TBS and incubated for 1 h with HRP-linked secondary

(4) Unraveling key genes and mitochondrial-related mechanisms of atherosclerosis severity: explorations based on interpretable machine learningBMC Cardiovascular DisordersFebruary 9, 2026De Zhao Kong, Xue Zhi Zhang, Yuan Yuan Zhou, Qun Wang, Yi Hui Pan, Yi Lu, Hui Ye, Xiong Yi Hong1:200), PMAIP1 (Santa Cruz Biotechnology, sc-56169, dilution 1:200), and GAPDH (Santa Cruz Biotechnology, sc-59540, dilution 1:500). In vivo validation Ten SPF-grade C57 mice were obtained from Liaoning Chansheng

(5) Endoplasmic reticulum stress contributes to insulin resistance in Hashimoto's ThyroiditisEndocrine ConnectionsAugust 1, 2025Qing Zhou, Li Yong Zhang, Xue Lin Gao, Chao Chun Zou, Hui Liuglyceraldehyde-3-phosphate dehydrogenase (GAPDH) was purchased from Santa Cruz Biotechnology (China, Cat#: sc-59540). GAPDH was used as the internal standard for total protein. Statistical analysis SPSS software (version

(6) Endoplasmic reticulum stress contributes to insulin resistance in Hashimoto’s thyroiditisEndocr ConnectJuly 31, 2025Qing Zhou, Li Yong Zhang, Xue Lin Gao, Chao Chun Zou, Hui Liuglyceraldehyde-3-phosphate dehydrogenase (GAPDH) was purchased from Santa Cruz Biotechnology (China, Cat#: sc-59540). GAPDH was used as the internal standard for total protein. Statistical analysis SPSS software (version

(7) Wnt/β-catenin signaling within multiple cell types dependent upon kramer regulates Drosophila intestinal stem cell proliferationiScienceJune 21, 2024Hongyan Sun, Adnan Shami Shah, Din-Chi Chiu, Alessandro Bonfini, Nicolas Buchon, Jeremy M. Baskin,Millipore Sigma,Cat#MAB131873 Anti-HA,Roche,"Cat#11867423001,RRID:AB_390918" Anti-GAPDH,Santa Cruz Biotechnology,"Cat# sc-59540,RRID:AB_631587" Anti-Rabbit IgG-HRP,Jackson ImmunoResearch Labs,"Cat# 111035144,RRID:AB_2307391" Anti-Mouse

(8) FOXO3a/PI3K/Akt pathway participates in the ROS- induced apoptosis triggered by α-ZEL and β-ZELScientific ReportsJune 10, 2024Dominika Ewa Habrowska-Górczyńska, Marta Justyna Kozieł, Kinga Anna Urbanek, Karolina Kowalska, Agnieszka Wanda Piastowska-Ciesielskamanufacturer’s recommendations. The results were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (sc-59540) (Santa Cruz Biotechnology Inc, Dallas, TX, USA) as a reference protein. Statistical analysis Results

(9) Compromised diabetic heart function is not affected by miR-378a upregulation upon hyperglycemiaPharmacological ReportsDecember 1, 2023Urszula Florczyk-Soluch, Katarzyna Polak, Reece Sabo, Alicja Martyniak, Jacek Stępniewski, Józef Dulak(ERK1/2) (1:500, Cell Signaling, cat. no. 9102), GAPDH (1:1000, Santa Cruz Biotechnology, cat. no. sc-59540) and HRP-conjugated secondary antibodies: anti-rabbit (1:5000, Cell Signaling, cat. no. 7074P2), anti-mouse

(10) Towards Cancer Nanoradiopharmaceuticals-Radioisotope Nanocarrier System for Prostate Cancer Theranostics Based on Radiation-Synthesized Polymer NanogelsCancersNovember 29, 2023Rurarz BP, Urbanek KA, Karczmarczyk U, Raczkowska J, Habrowska-Górczyńska DE, Kozieł MJ, Kowalska K, Kadłubowski S, Sawicka A, Maurin M, et al.Antibody,Secondary Antibody GAPDH,"Mouse monoclonal anti-GAPDH antibody (overnight at 4 °C, 1:10,000 dilution; sc-59540, Santa Cruz Biotechnology Inc., Dallas, TX, USA)","Alkaline phosphatase-conjugated goat anti-mouse polyclonal

(11) Rapamycin downregulates α-klotho in the kidneys of female rats with normal and reduced renal functionPLOS ONENovember 28, 2023Azahara Espartero, Angela Vidal, Ignacio Lopez, Ana I. Raya, Mariano Rodriguez, Escolastico Aguilera-Tejero, Carmen PinedaECL Western Blotting Detection Substrate (Thermo Fisher Scientific, Waltham, MA 02451 USA). GAPDH (sc-59540; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) was used as a housekeeping protein to ensure equal

(12) Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer CellsInternational journal of molecular sciencesMay 30, 2023Urbanek KA, Kowalska K, Habrowska-Górczyńska DE, Kozieł MJ, Domińska K, Piastowska-Ciesielska AW.the manufacturer’s instructions: CAV-1 (sc-894, Santa Cruz Biotechnology, Dallas, TX, USA), GAPDH (sc-59540, Santa Cruz Biotechnology, Dallas, TX, USA), and Cleaved Caspase-3 (mAb #9664, CST, Danvers, MA, USA).

(13) Delayed administration of Trichilia catigua A. Juss. Ethyl-acetate fraction after cerebral ischemia prevents spatial memory deficits, decreases oxidative stress, and impacts neural plasticity in ratsJournal of ethnopharmacologyApril 24, 2023de Oliveira DV, Godinho J, de Sa-Nakanishi AB, Comar JF, de Oliveira RMW, Bonato JM, Chinen LY, de Paula MN, Mello JCP, Previdelli IS, et al.

(14) ETV4 mediates dosage-dependent prostate tumor initiation and cooperates with p53 loss to generate prostate cancerScience advancesApril 5, 2023Li D, Zhan Y, Wang N, Tang F, Lee CJ, Bayshtok G, Moore AR, Wong EWP, Pachai MR, Xie Y, et al.anti–glyceraldehyde-3-phosphate dehydrogenase (1:10,000 for Western blotting; Santa Cruz Biotechnology, sc-59540). Tissue paraffin embedding, sectioning, and hematoxylin and eosin (H&E) staining were performed by

(15) Antineoplastic Nature of WWOX in Glioblastoma Is Mainly a Consequence of Reduced Cell Viability and InvasionBiologyMarch 17, 2023Kałuzińska-Kołat Ż, Kośla K, Kołat D, Płuciennik E, Bednarek AK.substrate (Invitrogen). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a reference protein (sc-59540, Santa Cruz Biotechnology Inc., Dallas, TX, USA). The relative protein amount was assessed with ImageJ

(16) Verteporfin-induced proteotoxicity impairs cell homeostasis and survival in neuroblastoma subtypes independent of YAP/TAZ expressionScientific reportsMarch 7, 2023Condurat AL, Aminzadeh-Gohari S, Malnar M, Schider N, Opitz L, Thomas R, Menon V, Kofler B, Pruszak J.

(17) Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigsApplied microbiology and biotechnologyFebruary 1, 2023Ranjitha HB, Dhanesh VV, Hosamani M, Sreenivasa BP, Jabeen U, Biswal JK, Saravanan P, Sanyal A, Bhanuprakash V, Basagoudanavar SH.3B-specific mAb (10H9D8), rabbit serum against serotype O capsid antigen or GAPDH (Santa Cruz, USA, #SC-59540) (dilution 1:500 for all), followed by species-specific HRP-conjugated secondary antibody (1:1000) (Dako,

(18) TGFα-EGFR pathway in breast carcinogenesis, association with WWOX expression and estrogen activationJournal of applied geneticsMay 1, 2022Pospiech K, Orzechowska M, Nowakowska M, Anusewicz D, Płuciennik E, Kośla K, Bednarek AK.reference, mouse anti-GAPDG monoclonal antibody was used (1:1000; Santa Cruz Biotechnology, cat. no. sc-59540). The relative protein amount was assessed with ImageJ (Schneider et al. 2012) based on the integrated

(19) Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder CancerCellsApril 19, 2022Kołat D, Kałuzińska Ż, Bednarek AK, Płuciennik E.was analyzed using ImageJ software and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH; sc-59540; Santa Cruz Biotechnology, Dallas, TX, USA) or β-actin (ACTB; GTX109639; GeneTex, Irvine, CA, USA).

(20) miR-378 affects metabolic disturbances in the mdx model of Duchenne muscular dystrophyScientific reportsMarch 10, 2022Podkalicka P, Mucha O, Kaziród K, Szade K, Stępniewski J, Ivanishchuk L, Hirao H, Pośpiech E, Józkowicz A, Kupiec-Weglinski JW, et al.Technology, Danvers, MA, USA/7074" Mouse anti-porcine GAPDH/1:2000,"Santa Cruz Biotechnology, Dallas TX, USA/sc-59540",Goat anti-mouse/1:20000,"BD Pharmingen, San Diego, CA, USA/554002" Rabbit anti-rat GLUT4/1:1000,"Abcam,

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GAPDH (1D4) Antibody

GAPDH (1D4) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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GAPDH (1D4) Antibody from Santa Cruz Biotechnology, Inc.

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