Fig 2: Muscleblind-like 1 (MBNL1) induces Snail mRNA decay by recruiting Snail transcripts to P-bodies. (A) RNP-IP assay. The association of Snail transcripts with MBNL1 was significantly enhanced by MBNL1 overexpression compared with vector. Data are presented as the means ± SD from triplicates in one representative experiment. ***P<0.001. Three experiments were performed that yielded similar results. (B) Snail protein levels. The protein levels of Snail were time-dependently downregulated by MBNL1 overexpression. (C) Snail mRNA levels. Data are presented as the means ± SE from 3 independent experiments. **P<0.01 vs. vector group. (D) mRNA turnover assay and half-time of Snail transcripts. Data are presented as the means ± SD from triplicates in one representative experiment. **P<0.01. Three experiments were performed that showed similar results. (E) RNP-IP assay. The association of Snail transcripts with Ago2 and RCK was significantly enhanced by MBNL1 overexpression compared with vector. Data are presented as the means ± SD from triplicates in one representative experiment. **P<0.01. (F) Rescue experiment. The indicated proteins were analyzed at 24 h following transfection. Notably, changes in Snail expression were significantly abolished by the depletion of Ago2 and RCK.