CD8 (32-M4) Antibody sc-1177 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemCD8 (32-M4) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-1177
Antibody TypePrimary Antibodies
NCBI Gene AliasesLyt-2, LY3, Ly-B, Ly-C, Ly-35, CD8alpha, p32, CD8beta, Leu2, CD8, CD8B1, Ly-2, LEU2, Lyt-3, Cd8b, Ly-3, P37, LYT3
ApplicationsWB, IP, IF, FCM
Quantity200 µg/ml
Clone32-M4
Reactivitymouse, rat, human
ClonalityMonoclonal
Hostmouse
Conjugate/TagUnconjugated
FormatPurified
NCBI Full Gene NameCD8 subunit beta 1, CD8 subunit beta, CD8 subunit alpha
TargetCD8
IsotypeIgG2a

Questions and Answers

Can this antibody be used for immunofluorescence staining in my tissue samples?

Yes, the CD8 (32-M4) antibody has been successfully used for immunofluorescence staining in various studies. For instance, in one study, the antibody was utilized to stain CD8+ T cells in tissue sections, demonstrating its effectiveness in visualizing these cells under fluorescence microscopy ().

What species does this antibody react with, and is it suitable for my mouse model?

The CD8 (32-M4) antibody is reactive with mouse, human, and rat species. Therefore, it is suitable for use in mouse models, as indicated by its application in studies involving murine tissues ().

Has this antibody been cited in any studies related to cancer research?

Yes, the CD8 (32-M4) antibody has been cited in multiple studies related to cancer research. For example, it was used in immunohistochemical analyses to assess CD8+ T cell infiltration in tumor tissues, which is crucial for understanding the immune response in cancer contexts (; ).

Is this antibody effective for Western blotting applications?

The CD8 (32-M4) antibody has been shown to work effectively in Western blotting applications, as evidenced by its use in various studies to detect CD8 protein levels in different experimental setups (; ).

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) CD80-Mediated T-Cell Suppression by Cancer Stem-like Cells in Head and Neck Squamous Cell CarcinomaCellsJanuary 30, 2026Mian Xiao, Lin Qiu, Qian Gao, Ruifeng Li, Jing Wang, Yanrui Feng, Xuefen Li, Xiyuan Gein the presence of a CD80-blocking antibody (2 mg/mL, Santa Cruz Biotechnology, Dallas, TX, USA, Cat#sc-1177). After 48 h, tumor cells were harvested for downstream analyses. To quantify live and dead cells, cells

(2) Mutual Exclusion Analysis Shows that DUSP9 Negatively Regulates PD‐L1 Expression and Acts as a Target to Enhance Anti‐PD‐1 EfficacyAdvanced ScienceDecember 17, 2025Yuzhe Hu, Ling Tang, Zheng Kuang, Danyi Huang, Ting Li, Gaofei Yin, Yingyu Chen, Wei Guo, Wenling Han, Pingzhang Wang(Cat#9134, Cell Signaling Technology), anti‐STAT3 (Cat#12640, Cell Signaling Technology), anti‐CD8 (Cat#sc‐1177, Santa Cruz), anti‐GZMB (Cat#13588‐1‐AP, Proteintech) and anti‐β‐actin (Cat#TA‐09, ZSGB‐Bio). Secondary

(3) Discovering molecular signatures in kidney transplant biopsies with borderline changes and isolated V-lesions: single-cell RNA-sequencing analysis of human blood and tissue Spatial transcriptomicsScientific reportsJuly 3, 2025Song J, Kang M, Jang Y, Joh CS, Shin HY, Baik JY, Choi HS, Kim KH, Lee SM, Cha SM, et al.antibodies diluted in 2% bovine serum albumin (BSA)/Tris-buffered saline (TBST) overnight at 4 ℃: anti-CD8B (sc-1177; Santa Cruz Biotechnology, dilution 1:50, [32-M4]) and anti-STAT1 (#14994; Cell Signaling Technology,

(4) Exosomes from Tregs mitigate lung damage caused by smoking via inhibiting inflammation and altering T lymphocyte subsets in COPD ratsBMC Pulm MedApril 14, 2025Xuefang Tao, Hai Tian, Guowen Wang, Yongzhen Sun, Liangyan Zhaoflow cytometry were as follows: CD4 antibody (sc-19641, Santa Cruz Biotechnology, USA; 1:20), CD8 antibody APC (sc-1177, Santa Cruz Biotechnology; 1:20), antibody FITC-CD25 (102005, Biolegend, China; 1:200), IL-17 PE antibody

(5) The role of TRAF2 in pan-cancer revealed by integrating informatics and experimental validationFront PharmacolMarch 12, 2025Xizheng Wang, Jianfeng Yuan, Chenchen Zhang, Lingyu Kong, Enzhen Wu, Jianxin Guo, Zhongbing Wusc-1178, Santa Cruz); CD3 (1:50, sc-20047, Santa Cruz); CD4 (1:50, sc-19641, Santa Cruz); and CD8 (1:50, sc-1177, Santa Cruz). Slides were stained with DAPI for cell nuclei labeling and observed using a fluorescence microscope

(6) KHSRP promotes cancer stem cell maintenance, tumorigenesis, and suppresses anti-tumor immunity in gastric cancerOncology researchJanuary 1, 2025DU Y, Pei Z, Hu S, Liao C, Liu S.temperature. Primary antibodies (The primary antibodies used are listed in Table S1) (CD8, Santa Cruz, sc-1177) diluted in blocking buffer were applied, and the samples were incubated overnight at 4°C. After washing

(7) Characterization of Immune Cell Infiltration and Collagen Type III Disorganization in Human Secondary Lymphedema: A Case-control StudyPlastic and Reconstructive Surgery - Global OpenJune 17, 2024Andreas Spörlein, Christoph Hirche, Juan Enrique Berner, Ulrich Kneser, Patrick A. Willat 4°C in a humid chamber for 20h: anti-CD3 (sc-20047, 1:100), anti-CD4 (sc-19641, 1:100), anti-CD8 (sc-1177, 1:100), anti-CD68 (sc-20060, 1:100), anti-CD163 (sc-20066, 1:100), anti-NOS2 (sc-7271, 1:100), from

(8) Circulating T cell specific extracellular vesicle profiles in cardiac allograft acute cellular rejectionAmerican Journal of TransplantationMarch 1, 2024Laxminarayana Korutla, Jessica R. Hoffman, Susan Rostami, Robert Hu, Varun Korutla, Caroline Markmann, Clancy Mullan, Alex Sotolongo, Andreas Habertheuer, Connie Romano, et al.,Santa Cruz Biotechnology,SC-8022,1:200 IFNy,Proteintech,15365-1-AP,1:500 FOXP3,Proteintech,22228-1-AP,1:500 Flotillin 1,Proteintech,15571-1-AP,1:500 MHC Class II,Abcam,Ab55152,1:500 CD8,Santa Cruz Biotechnology,SC-1177,1:200 SERCA2,Santa Cruz Biotechnology,SC-73022,1:200 CD63,Santa Cruz Biotechnology,SC-365604,1:200 Cytochrome

(9) CXCL12 Neutralizing Antibody Promotes Hair Growth in Androgenic Alopecia and Alopecia AreataInternational journal of molecular sciencesJanuary 30, 2024Zheng M, Kim MH, Park SG, Kim WS, Oh SH, Sung JH.,Santa cruz; sc-7305,1:100,1:1000,"DP, DF cell" CXCR4,NOVUS; NB100-56437,,1:1000,DP cell CD8,Santa cruz; sc-1177,1:100,,Mouse skin tissue MHC-I,Santa cruz; sc-55582,1:100,,Mouse skin tissue MHC-II,Santa cruz; sc-32247,1:100,,Mouse

(10) Glioblastoma cellular MAP4K1 facilitates tumor growth and disrupts T effector cell infiltrationLife science allianceDecember 1, 2023Sun JM, Fan HY, Zhu Y, Pan TT, Wu YP, Zhang DY, Hou XY.PBS containing 10% normal goat serum) for 40 min at RT. Mouse anti-CD8 monoclonal antibody (1:300, sc-1177; Santa Cruz) was added and incubated overnight at 4°C. Then, Alexa Fluor 594 goat anti-mouse secondary

(11) Combined Inhibition of Indolamine-2,3-Dioxygenase 1 and C-X-C Chemokine Receptor Type 2 Exerts Antitumor Effects in a Preclinical Model of Cervical CancerBiomedicinesAugust 16, 2023Lizcano-Meneses S, Hernández-Pando R, García-Aguirre I, Bonilla-Delgado J, Alvarado-Castro VM, Cisneros B, Gariglio P, Cortés-Malagón EM.(1:100) (Cat# BS-2549R; Bioss Antibodies, Woburn, MA, USA); CD8 (1:100), PCNA (1:100) (Cat# SC-25280 and SC-1177; respectively; Santa Cruz Biotechnology, Dallas, TX, USA); MPO (1:8000) and Granzyme B (1:100) (Cat#

(12) Engineering a facile and versatile nanoplatform to facilitate the delivery of multiple agents for targeted breast cancer chemo-immunotherapyBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapieJuly 1, 2023Bahreyni A, Mohamud Y, Zhang J, Luo H.

(13) A combination of genetically engineered oncolytic virus and melittin-CpG for cancer viro-chemo-immunotherapyBMC medicineMay 24, 2023Bahreyni A, Liu H, Mohamud Y, Xue YC, Fan YM, Zhang YL, Luo H.5 μm of thicknesses. The sections were deparaffinized, rehydrated, and then stained with anti-CD8 (sc-1177, Santa Cruz Biotechnology), anti-NK1.1 (14–5941-82, eBioscience), and anti-F4/80 (sc-377009, Santa Cruz Biotechnology

(14) New Insights into the Mechanism of Ulva pertusa on Colitis in Mice: Modulation of the Pain and Immune SystemMarine drugsMay 13, 2023Ardizzone A, Mannino D, Capra AP, Repici A, Filippone A, Esposito E, Campolo M.Cruz Biotechnology, Dallas, TX, USA; sc-13573), CD8 (1:100; Santa Cruz Biotechnology, Dallas, TX, USA; sc-1177), and CD68 (1:100; Santa Cruz Biotechnology, Dallas, TX, USA; sc-20060). After one night of incubation

(15) TRAF2 as a key candidate gene in clinical hepatitis B-associated liver fibrosisFrontiers in Molecular BiosciencesApril 5, 2023Cichun Wu, Jian Zhang, Huiwen Wang, Wei Zhang, Jingqing Liu, Nianqi Zhou, Keyu Chen, Ying Wang, Shifang Peng, Lei FuAbcam); anti-CD3 (1:50, sc-20047; Santa Cruz); anti-CD4 (1:50, sc-19641; Santa Cruz); and anti-CD8 (1:50, sc-1177; Santa Cruz), DAPI were used to label the cell nuclei to identify the cellular localization of TRAF2

(16) Loss of Peroxiredoxin IV Protects Mice from Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Cancer DevelopmentAntioxidants (Basel, Switzerland)March 9, 2023Thapa P, Jiang H, Ding N, Hao Y, Alshahrani A, Lee EY, Fujii J, Wei Q.

(17) Hepatocyte growth factor-mediated apoptosis mechanisms of cytotoxic CD8(+) T cells in normal and cirrhotic liversCell death discoveryJanuary 19, 2023Chen Q, Yan M, Lin H, Lai J, Yang Z, Hu D, Deng Y, Shi S, Shuai L, Zhang L, et al.State,Country Primary Abs,CD8α,14-0081-85,"WB, IHC, IF, FC, IP,Neu, FN",Invitrogen,California,USA Primary Abs,CD8,Sc-1177,"WB, IP, IF, FC",Santa Cruz Biotechnology,California,USA Primary Abs,Ki67,550609,"FC, IF",Biosciences,New

(18) Orthotopic and heterotopic triple negative breast cancer preclinical murine models: A tumor microenvironment comparativeResearch in veterinary scienceDecember 20, 2022Santana-Krímskaya SE, Kawas JR, Zarate-Triviño DG, Ramos-Zayas Y, Rodríguez-Padilla C, Franco-Molina MA.

(19) A new miRNA-Modified coxsackievirus B3 inhibits triple negative breast cancer growth with improved safety profile in immunocompetent miceCancer lettersNovember 1, 2022Bahreyni A, Liu H, Mohamud Y, Xue YC, Zhang J, Luo H.

(20) Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinomaWorld journal of surgical oncologyAugust 25, 2022Zheng E, Cai Z, Li W, Ni C, Fang Q.anti-CD206 (ab64693, Abcam, Cambridge, USA); anti-CD163 (ab182422, Abcam, Cambridge, USA); anti-CD8a (sc-1177, Santa Cruz Biotechnology, Heidelberg, Germany); anti-CD109 (sc-271085, Santa Cruz Biotechnology, Heidelberg,

Be the First to Write a Review!

CD8 (32-M4) Antibody

CD8 (32-M4) Antibody from Santa Cruz Biotechnology, Inc.

Description

Questions and Answers

Santa Cruz Biotechnology, Inc.

Citations (42)

Reviews

CD8 (32-M4) Antibody from Santa Cruz Biotechnology, Inc.

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map