Cdc27 (AF3.1) Antibody sc-9972 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemCdc27 (AF3.1) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-9972
Antibody TypePrimary Antibodies
NCBI Gene AliasesHNUC, NUC2, D0S1430E, H-NUC, CDC27Hs, APC3, ANAPC3, D17S978E
ApplicationsWB, IP, IF, IHC(P)
Reactivitymouse, rat, human
CloneAF3.1
Quantity200 µg/ml
ClonalityMonoclonal
Hostmouse
Conjugate/TagUnconjugated
FormatPurified
NCBI Full Gene Namecell division cycle 27
TargetCdc27
IsotypeIgG2b

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) CCT5 maintains mitotic fidelity and promotes early colorectal tumorigenesisiScienceApril 17, 2026Meijun Ji, Wenhan Zhuang, Yumin Guo, Pengfei Xu, Xiaolu Zhou, Yan Long, Xiaoge Geng, Jiyong Jing, Xuelong Zhou, Wensheng Pan, et al.,Santa Cruz Biotechnology,"Cat# sc-13162, RRID:AB_628089" Mouse monoclonal anti-CDC27 (APC3),Santa Cruz Biotechnology,"Cat# sc-9972, RRID:AB_627228" Rabbit monoclonal anti-Securin,Abcam,"Cat# ab79546, RRID:AB_2173411" Rabbit monoclonal

(2) APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degronsNat CommunMarch 15, 2025Aleksandra Skrajna, Tatyana Bodrug, Raquel C. Martinez-Chacin, Caleb B. Fisher, Kaeli A. Welsh, Holly C. Simmons, Eyla C. Arteaga, Jake M. Simmons, Mohamed A. Nasr, Kyle M. LaPak, et al.was later performed with the same membrane using a mouse anti-APC3 antibody (1:10,000, Santa Cruz, SC-9972) and 680CW goat anti-mouse IgG secondary antibody (1:10,000, LI-COR) allowing simultaneous detection

(3) Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinomaScientific ReportsOctober 15, 2024Zehua Lin, Weisong Cai, Yuechen Sun, Baoai Han, Yifan Hu, Shuo Huang, Jun Li, Xiong Chencat.16960 S; Cell Signaling Technology, Danvers, MA, US), anti-CDC27 antibodies (dilution 1:500; cat.sc-9972; Santa Cruz Biotechnology, Dallas, Texas, US), and anti-cyclin B1 (dilution, 1:100; cat.4-138T; Cell

(4) CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCCAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2024Li Q, Chen Q, Zheng T, Wang F, Teng J, Zhou H, Chen J.1:500), anti‐CDC20 (Santa Cruz Biotechnology, sc‐13162, 1:400), anti‐APC3 (Santa Cruz Biotechnology, sc‐9972, 1:300), anti‐APC2 (Proteintech, 13559‐1‐AP, 1:500), anti‐APC5 (Proteintech, 67348‐1‐Ig, 1:500), anti‐APC4

(5) SETDB2 interacts with BUBR1 to induce accurate chromosome segregation independently of its histone methyltransferase activityFEBS open bioMarch 1, 2024Tu Y, Zhang H, Xia J, Zhao Y, Yang R, Feng J, Ma X, Li J.Proteintech, Wuhan, China), MAD2 (#66014‐1‐Ig; Proteintech), MPS1 (#ab11108; Abcam, Cambridge, UK), APC3 (#sc‐9972; Santa Cruz Biotechnology, Dallas, TX, USA), and CYCLIN B1 (#A2056; ABclonal Technology, Wuhan, China)

(6) Lactate regulates cell cycle by remodelling the anaphase promoting complexNatureApril 1, 2023Liu W, Wang Y, Bozi LHM, Fischer PD, Jedrychowski MP, Xiao H, Wu T, Darabedian N, He X, Mills EL, et al.immunoprecipitated from mitotic cell lysates overnight at 4 °C with the anti-CDC27 (AF3.1) antibody (Santa Cruz, sc-9972; 2 μg of antibody per sample) that was coated on protein G magnetic beads (Invitrogen, 10007D) in the

(7) Proximity Labeling Reveals Spatial Regulation of the Anaphase-Promoting Complex/Cyclosome by a Microtubule AdaptorACS chemical biologySeptember 16, 2022Cao X, Shami Shah A, Sanford EJ, Smolka MB, Baskin JM.

(8) Role of phosphorylation of Cdc20 in the regulation of the action of APC/C in mitosisProceedings of the National Academy of Sciences of the United States of AmericaAugust 30, 2022Shevah-Sitry D, Miniowitz-Shemtov S, Teichner A, Kaisari S, Hershko A.

(9) Allosteric control of Ubp6 and the proteasome via a bidirectional switchNature communicationsFebruary 11, 2022Hung KYS, Klumpe S, Eisele MR, Elsasser S, Tian G, Sun S, Moroco JA, Cheng TC, Joshi T, Seibel T, et al.

(10) Protein kinase RNA-activated controls mitotic progression and determines paclitaxel chemosensitivity through B-cell lymphoma 2 in ovarian cancerOncogeneDecember 1, 2021Yin L, Zeng Y, Zeng R, Chen Y, Wang TL, Rodabaugh KJ, Yu F, Natarajan A, Karpf AR, Dong J.antibody (9116, 1:1000) was from Cell Signaling Technology. Anti-cyclin B1 (sc-245, 1:2000), anti-CDC27 (sc-9972, 1:1000), anti-GFP (sc-9996, 1:2000), and anti-β-actin (sc-47778, 1:2000) antibodies were obtained from

(11) Mechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO-RNF4 pathwayThe EMBO journalSeptember 15, 2021Liu JCY, Kühbacher U, Larsen NB, Borgermann N, Garvanska DH, Hendriks IA, Ackermann L, Haahr P, Gallina I, Guérillon C, et al.(1:1,000), RRID:AB_306089); ATM‐pS1981 (4526, Cell Signaling Technology (1:1,000), RRID:AB_2062663); CDC27 (sc‐9972, Santa Cruz, RRID:AB_627228); CDK1‐pY15 (ab47594, Abcam (1:1,000), RRID:AB_869073); CHK1 (sc‐8408, Santa Cruz

(12) Reciprocal interaction between SIRT6 and APC/C regulates genomic stabilityScientific reportsJuly 9, 2021Wang H, Feng K, Wang Q, Deng H.(ab79546) were purchased from Abcam (Cambridge, MA, USA). Mouse anti-SIRT6 (sc-517196), mouse anti-Cdc27 (sc-9972), and mouse anti-CDC20 (sc-13162) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA,

(13) Elucidating Human Mitosis Using an Anaphase-Like Cell-Free SystemMethods in molecular biology (Clifton, N.J.)January 1, 2021Wasserman D, Nachum S, Noach-Hirsh M, Auerbach N, Sheinberger-Chorni E, Enrico TP, Lahmi R, Emanuele MJ, Tzur A.

(14) FLEXIQuant-LF to quantify protein modification extent in label-free proteomics dataeLifeDecember 7, 2020Schlaffner CN, Kahnert K, Muntel J, Chauhan R, Renard BY, Steen JA, Steen H.,HeLa S3,ATCC,"Cat# CCL-2.2, RRID:CVCL_0058", Antibody,Mouse monoclonal CDC27 antibody (AF3.1),Santa Cruz,Cat# sc-9972,IP (1:3) Antibody,Mouse polyclonal Normal mouse IgG,Santa Cruz,Cat# sc-2025,IP (1:6) "Peptide, recombinant

(15) Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome bindingNucleic acids researchSeptember 25, 2020Skrajna A, Goldfarb D, Kedziora KM, Cousins EM, Grant GD, Spangler CJ, Barbour EH, Yan X, Hathaway NA, Brown NG, et al.ABclonal, A11629), rabbit anti-AHCTF1 (1:150, Sigma, HPA031658), mouse anti-CDC27 (1:375, Santa Cruz, sc-9972), rabbit anti-ACTR6 (1:1000, ABclonal, A13227), rabbit anti-CDC16 (1:500, ABclonal, A7197), rabbit anti-SEC13

(16) Kv10.1 Regulates Microtubule Dynamics during MitosisCancersAugust 25, 2020Movsisyan N, Pardo LA.,Merck Millipore p-histone H2AX (Ser139),Rb/mcl,1:1000,sc-101696,Cell Signaling CDC27 (AF3.1),Ms/mcl,1:1000,sc-9972,Santa Cruz Biotechnology CDC20,Rb/pcl,1:1000,ab26483,Abcam AURKB/AIM1,Rb/pcl,1:1000,3094,Cell Signaling PLK1,Ms/mcl,1:500,sc-17783,Santa Cruz Biotechnology

(17) Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Is Independent of Anaphase-Promoting Complex ActivityJournal of virologyJune 16, 2020Elbasani E, Gramolelli S, Günther T, Gabaev I, Grundhoff A, Ojala PM.(sc-166714), CCNB1 (sc-245), ORF57 (sc-135746), ORF45 (sc-53883), K8.1 (sc-65446), actin (sc-8432), APC3 (sc-9972), securin (sc-56207), CCNA2 (sc-271682), PLK1 (sc-17783), and p53 (sc-126) were from Santa Cruz Biotechnology

(18) Paradoxical mitotic exit induced by a small molecule inhibitor of APC/C(Cdc20)Nature chemical biologyMay 1, 2020Richeson KV, Bodrug T, Sackton KL, Yamaguchi M, Paulo JA, Gygi SP, Schulman BA, Brown NG, King RW.anti-mouse IgG Alexa Fluor 633 (A-21052, Invitrogen). For APC/C immunoprecipitation, anti-Cdc27 (AF3.1: sc-9972, Santa Cruz Biotechnology) was used; for BubR1 immunoprecipitation, anti-BubR1 (612502, BD Transduction

(19) Cell cycle oscillators underlying orderly proteolysis of E2F8Molecular biology of the cellApril 1, 2020Wasserman D, Nachum S, Cohen M, Enrico TP, Noach-Hirsh M, Parasol J, Zomer-Polak S, Auerbach N, Sheinberger-Chorni E, Nevenzal H, et al.(Santa Cruz Biotechnology; #SC-8358), anti-Cdh1 (Calibochem; #CC43-100UG), anti-Cdc27 (Santa Cruz Biotechnology #SC-5618 and SC-9972), anti-Cyclin F (Santa Cruz Biotechnology; #SC-952), and anti-HA (Biolegend; #901502); anti-FLAG (Sigma;

(20) Gene expression and cell identity controlled by anaphase-promoting complexNatureMarch 1, 2020Oh E, Mark KG, Mocciaro A, Watson ER, Prabu JR, Cha DD, Kampmann M, Gamarra N, Zhou CY, Rape M.

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Cdc27 (AF3.1) Antibody

Cdc27 (AF3.1) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Cdc27 (AF3.1) Antibody from Santa Cruz Biotechnology, Inc.

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