GATA-4 (G-4) Antibody from Santa Cruz Biotechnology, Inc.

The GATA-4 antibody has been shown to work in various applications, including Western Blotting, ELISA, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. It has been utilized in studies to detect endoderm markers and assess cardiac cell fate, as well as in the differentiation of induced pluripotent stem cells into cholangiocyte-like cells.

Yes, the GATA-4 antibody is reactive with multiple species, including humans, mice, and rats. This broad reactivity allows researchers to utilize it in comparative studies across different animal models.

Yes, one study reported that GATA4 is a substrate for caspase-1, which cleaves GATA4 and generates a truncated protein that acts as a dominant negative regulator. This finding highlights the role of GATA4 in cardiomyocyte survival and its involvement in heart failure mechanisms (aries2014).

The GATA-4 antibody has been employed in the differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells, demonstrating its utility in studying biliary development and related disorders (sampazio2017). Additionally, it has been used in immunostaining to assess pluripotency and lineage markers in stem cell-derived organoids (koike2021).