TNF-alpha Antibody MAB4101 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTNF-alpha Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberMAB4101
ApplicationsBioassay,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,In vivo assay,Neutralization,Intracellular Staining by Flow Cytometry,Cell Culture,ELISA Capture
ReactivityHuman,Mouse,Transgenic Mouse
IsotypeIgG1
CloneMP6-XT22
TargetTNF-alpha
HostRat
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
ClonalityMonoclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Immunogenmouse TNF-alpha
ConcentrationLYOPH
PurityProtein A or G purified from hybridoma culture supernatant
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesDIF, TNF-alpha, TNFA, TNFSF2, TNLG1F

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse TNF-alpha.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancerCell Oncol (Dordr)March 23, 2026Hongxu Zhou, Chengzhao Xu, Jiabeini Zhang, Yangxuzhu Liu, Xiaochuan Gao, Ziqi He, Yuheng Wu, Qian Zhao, Baoling Liang, Dong SongAB_2233899), anti-CXCL1 (R&D systems, Cat. MAB453; RRID: AB_2087696), and anti-TNF-α (R&D systems, Cat. MAB4101; RRID: AB_2240643). Recombinant IL-6 protein was purchased from R&D systems (Cat. 406-ML). Animal experiments For

(2) Retinal pigment epithelial cells reduce vascular leak and proliferation in retinal neovesselsAngiogenesisMarch 1, 2025Simone Tzaridis, Edith Aguilar, Michael I. Dorrell, Martin Friedlander, Kevin T. Eadeneedle (Hamilton), injecting 1 µl of solutions containing neutralizing antibodies against TNFa (250ng; MAB4101; R&D systems), FGF2 (200ng; clone bFM-1; 05-117, Millipore Sigma) or VEGF164 (200ng; AF-493-NA; R&D systems

(3) Therapeutic single-cell landscape: methotrexate exacerbates interstitial lung disease by compromising the stemness of alveolar epithelial cells under systemic inflammationeBioMedicineOctober 1, 2024Sung Hae Chang, Seyoung Jung, Jeong Jun Chae, Jeong Yeon Kim, Seon Uk Kim, Ji Yong Choi, Hye-Jeong Han, Hyun Taek Kim, Hak-Jae Kim, Hyun Je Kim, et al.intraperitoneal injections of PBS, MTX (7.5 mg/kg; MilliporeSigma, M9929), or TNFα inhibitor (100 μg/kg; R&D Systems, MAB4101). Each group consisted of four mice, with each group maintained in separate cages and housed in the

(4) Functionalized nanowires for miRNA-mediated therapeutic programming of naïve T cellsNature nanotechnologyAugust 1, 2024Yee Mon KJ, Kim S, Dai Z, West JD, Zhu H, Jain R, Grimson A, Rudd BD, Singh A.

(5) Influenza virus infection activates TAK1 to suppress RIPK3-independent apoptosis and RIPK1-dependent necroptosisCell Communication and SignalingJuly 23, 2024Yuling Sun, Lei Ji, Wei Liu, Jing Sun, Penggang Liu, Xiaoquan Wang, Xiufan Liu, Xiulong XuImmunoResearch (West Grove, PA, USA). Rat IgG1 isotype control (MAB005) and Rat anti-mouse TNF-α antibody (MAB4101) were purchased from R&D Systems (Minneapolis, MN, USA). Cell culture LET1 cells, a murine lung epithelial

(6) Embryonic vitamin D deficiency programs hematopoietic stem cells to induce type 2 diabetesNature communicationsJune 13, 2023Oh J, Riek AE, Bauerle KT, Dusso A, McNerney KP, Barve RA, Darwech I, Sprague JE, Moynihan C, Zhang RM, et al.

(7) Targeting WDxR motif reprograms immune microenvironment and inhibits hepatocellular carcinoma progressionEMBO molecular medicineMay 8, 2023Zhang H, Chen G, Feng X, Song H, Meng L, Fu Y, Yang J, Fan Z, Ding Y, Du Z, et al.A5316‐100ul, 1:3,000), anti‐pp65 (Cell Signaling Technologies, #13346, 1:1,000), anti‐TNF‐α (R&D Systems, MAB4101, 1:2,000), anti‐DDB1 (Abcam, ab109027, 1:2,000), IgG (R&D Systems, MAB005, 1:2,000), anti‐GAPDH (Santa Cruz Biotechnology

(8) Severe influenza infection is associated with inflammatory programmed cell death in infected macrophagesFrontiers in cellular and infection microbiologyJanuary 1, 2023Ferreira AC, Sacramento CQ, Pereira-Dutra FS, Fintelman-Rodrigues N, Silva PP, Mattos M, de Freitas CS, Marttorelli A, de Melo GR, Campos MM, et al.,R&D System,410-MT/CF Resatorvid,CLI95,TLR4,2 µM,In vivogen,tlrl-cli95 anti-TNF antibody,anti-TNF,TNF,1 ng/mL,R&D System,MAB4101 Polymyxin b,POLI,LPS,100 ng/mL,Sigma−Aldrich,P4932 Lipopolysaccharide serotype 0111: B4,LPS,TLR4,10

(9) Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1β to downregulate PPARα and FOXO3Cell reportsJanuary 25, 2022Li Y, Zhu Y, Feng S, Ishida Y, Chiu TP, Saito T, Wang S, Ann DK, Ou JJ.

(10) EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathwayFrontiers in immunologyJanuary 1, 2022Wu J, Luo FL, Xie Y, Xiong H, Gao Y, Liu G, Zhang XL.and then purified EST12 (2 μM) was added with or without the presence of TNF-α neutralizing antibody (MAB4101, R&D Systems, USA) (10 μg/ml) and further incubated for 8 h. Cells were lysed and intracellular bacteria

(11) Activating a collaborative innate-adaptive immune response to control metastasisCancer cellOctober 11, 2021Sun L, Kees T, Almeida AS, Liu B, He XY, Ng D, Han X, Spector DL, McNeish IA, Gimotty P, et al.polyclonal anti-Mouse IL-12,R&D Systems,Cat #AF-419-NA Rat monoclonal anti-Mouse TNF-alpha,R&D Systems,Cat #MAB4101 Goat polyclonal anti-Mouse CXCL9/MIG,R&D Systems,Cat #AF-492-NA InVivoMAb anti-mouse CD8α,BioXCell,Cat

(12) ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in MacrophagesArteriosclerosis, thrombosis, and vascular biologyJune 1, 2021Kothari V, Tang J, He Y, Kramer F, Kanter JE, Bornfeldt KE.neutralization experiments, BMDMs were treated with a neutralizing anti-TNFα antibody (10 μg/ml; R&D systems; MAB4101) or isotype control (10 μg/ml; R&D systems; MAB005) for 4 hr to block the response of shed TNFα. For

(13) A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammationImmunityFebruary 9, 2021Liu Z, Nailwal H, Rector J, Rahman MM, Sam R, McFadden G, Chan FK.GSK’872 (kind gift of GSK, 1 μM), Nec1s (APExBIO, 1 μM) or TNF neutralizing antibody (R&D Systems, MAB4101 at 10 μg/ml). Cell death was tracked by the Incucyte live cell imaging using 100 nM Cytotox Red (Essen

(14) ABIN-1 is a key regulator in RIPK1-dependent apoptosis (RDA) and necroptosis, and ABIN-1 deficiency potentiates necroptosis-based cancer therapy in colorectal cancerCell death & diseaseFebruary 1, 2021Cai J, Hu D, Sakya J, Sun T, Wang D, Wang L, Mao X, Su Z.9542); cleaved caspase-3 (Cell Signaling, Cat# 9661); TNF neutralization antibody (R&D Systems, Cat#MAB4101); β-actin (Proteintech Cat# 20536-1-AP); TNFR1 (Santa Cruz Biotechnology, Cat# sc-8436); cIAP1 (Cell Signaling

(15) Spinal interleukin-6 contributes to central sensitisation and persistent pain hypersensitivity in a model of juvenile idiopathic arthritisBrain, behavior, and immunityNovember 1, 2020Kwok CHT, Learoyd AE, Canet-Pons J, Trang T, Fitzgerald M.necrosis factor-alpha (TNFα) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (MAB406, MAB401, MAB4101, AF5718, R&D Systems, UK; 1:500 in 0.2% i-block solution). Primary antibodies were detected using horseradish

(16) Myeloma Cells Down-Regulate Adiponectin in Bone Marrow Adipocytes Via TNF-AlphaJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchMay 1, 2020Morris EV, Suchacki KJ, Hocking J, Cartwright R, Sowman A, Gamez B, Lea R, Drake MT, Cawthorn WP, Edwards CM.neutralization Cytokine blocking was achieved using an antibody directed against mouse TNF‐α (R&D Systems MAB4101). Co‐cultures of myeloma cells and mature adipocytes were treated with anti‐TNF‐α at a concentration

(17) Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapyNature communicationsNovember 28, 2019Shi L, Wang J, Ding N, Zhang Y, Zhu Y, Dong S, Wang X, Peng C, Zhou C, Zhou L, et al.isolated from residual tumors) at 37 °C for 6–48 h. To block TNFα and TNFR1, 2 μg anti-mouse TNFα IgG (Cat MAB4101, R&D Systems) or anti-TNFR1 mAb (Cat MAB430-100, R&D Systems) was added to each well. The level of CCL2

(18) Tumor Necrosis Factor-α-Mediated Metaplastic Inhibition of LTP Is Constitutively Engaged in an Alzheimer's Disease ModelThe Journal of neuroscience : the official journal of the Society for NeuroscienceNovember 13, 2019Singh A, Jones OD, Mockett BG, Ohline SM, Abraham WC.pulses. Anti-rat TNF␣ Ab (catalog #MAB510), anti-rat IL-1␤ Ab (catalog #MAB501), anti-mouse TNF␣ Ab (catalog #MAB4101), and anti-mouse IL-1␤ Ab (catalog #MAB401) were purchased from R&D Systems. Thalidomide, SB203580,

(19) Iron alters macrophage polarization status and leads to steatohepatitis and fibrogenesisJournal of leukocyte biologyMay 1, 2019Handa P, Thomas S, Morgan-Stevenson V, Maliken BD, Gochanour E, Boukhar S, Yeh MM, Kowdley KV.

(20) Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARCOncogeneMay 1, 2019John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, Said N.

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TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody

TNF-alpha Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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TNF-alpha Antibody from R&D Systems, a Bio-Techne Brand

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